Ansam_00320 Ansam_00320   Ansam_00340 Ansam_00340

Ansam_00330 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction73,083 / 72,502 / - [in whole cluster]
9,664 / 10,245 / + [in contig]
Locationcomplement(72502..73083) [in whole cluster]
9664..10245 [in contig]
TypeCDS
Length582 bp (193 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative TetR family transcriptional regulator
Product (GenBank)transcriptional regulator
Gene
Gene (GenBank)asm29
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
[19609582] Constitutive overexpression of asm2 and asm39 increases AP-3 production in the actinomycete Actinosynnema pretiosum. (J Ind Microbiol Biotechnol. , 2009)
comment
[PMID:12060743](2002)
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
Asm29(193aa) : Transcriptional regulator(Function of homologue)

本文にコメントなし。

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[PMID:19609582](2009)
putative transcriptional regulators asm2, asm29, and asm34と、putative regulatory protein asm39をsingle-site integrative vector pAP40 と multicopy replicative vector pREPにクローニング。
これをA. pretiosumへ戻して恒常的過剰産生させ、ansamitocin産生量への影響を見ている。

H. avellaneaへの感受性に基づいたansamitocin bioassayでの抑制指数は、replicative vectorでのasm29過剰発現で1.1倍の控えめな増加を示した。integrative vectorでは有意差なし。

自発的な高産生株A. pretiosum mutantではasm29がupregulateされていることがマイクロアレイ解析で示されている(unpublished data)。
Family/Domain
FD
IPR001647
comment
[IPR001647] DNA-binding HTH domain, TetR-type (Domain)

close this sectionSequence

selected fasta
>putative TetR family transcriptional regulator [transcriptional regulator]
MSAAVRREQLVAAALRVMKRDGIAAATTRAICAEADMPHGAFHYCFRSKQELYTALLTTG
LNTSLDAAWPAISPDLPAVDNVRTMLAAFWTAFQADPEAELVLGELVTLAQREPELRELP
AWQHREYVAKAVAHLDRLAEEAGFDYSVDRALLAELVVSVLDGVASSWFSHRDGELAKAS
LDQFAVMFAALTR
selected fasta
>putative TetR family transcriptional regulator [transcriptional regulator]
GTGAGCGCGGCCGTGCGGCGCGAGCAGCTCGTGGCCGCCGCGCTGCGCGTGATGAAGCGC
GACGGGATCGCCGCGGCGACCACGCGCGCGATCTGCGCCGAGGCCGACATGCCGCACGGC
GCGTTCCACTACTGCTTCCGCTCCAAGCAGGAGCTGTACACCGCGCTGCTCACCACGGGC
CTGAACACCAGCCTGGACGCGGCGTGGCCCGCGATCTCGCCGGACCTGCCCGCCGTGGAC
AACGTCCGCACGATGCTGGCCGCCTTCTGGACCGCGTTCCAGGCCGACCCGGAGGCGGAG
CTGGTGCTGGGCGAGCTGGTCACCCTCGCCCAGCGGGAGCCCGAGCTGCGCGAGCTGCCC
GCGTGGCAGCACCGCGAGTACGTCGCCAAGGCGGTCGCGCACCTGGACCGGCTCGCCGAG
GAGGCCGGGTTCGACTACTCGGTGGACCGGGCGCTGCTGGCGGAGCTGGTGGTGTCGGTG
CTGGACGGGGTCGCCTCGTCCTGGTTCTCGCACCGGGACGGGGAGCTGGCGAAGGCGTCG
CTGGACCAGTTCGCCGTGATGTTCGCGGCCCTGACGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001647 DNA-binding HTH domain, TetR-type (Domain)
[4-64] PS50977
PS50977   HTH_TETR_2
[10-56] 7e-12 PF00440
PF00440   TetR_N
IPR009057 Homeodomain-like (Domain)
[2-80] 4.79999776836274e-15 SSF46689
SSF46689   Homeodomain_like
IPR011075 Tetracycline transcriptional regulator, TetR-related, C-terminal (Domain)
[76-193] 2.70000183580794e-10 SSF48498
SSF48498   TetR_like_C
IPR015893 Tetracycline transcriptional regulator, TetR-like, C-terminal (Domain)
[5-187] 6.19999999999995e-33 G3DSA:1.10.357.10
G3DSA:1.10.357.10   TetR_C
SignalP
[1-24] 0.439 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Ansam_00320 Ansam_00320   Ansam_00340 Ansam_00340
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