Ansam_00370 Ansam_00370   Ansam_00390 Ansam_00390

Ansam_00380 : CDS information

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Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction78,683 / 79,297 / + [in whole cluster]
4,064 / 3,450 / - [in contig]
Location78683..79297 [in whole cluster]
complement(3450..4064) [in contig]
TypeCDS
Length615 bp (204 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative TetR family transcriptional regulator
Product (GenBank)transcriptional regulator
Gene
Gene (GenBank)asm34
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
[19609582] Constitutive overexpression of asm2 and asm39 increases AP-3 production in the actinomycete Actinosynnema pretiosum. (J Ind Microbiol Biotechnol. , 2009)
comment
[PMID:12060743](2002)
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
Asm34(204aa) : Transcriptional regulator(Function of homologue)

本文にコメントなし。

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[PMID:19609582](2009)
putative transcriptional regulators asm2, asm29, and asm34と、putative regulatory protein asm39をsingle-site integrative vector pAP40 と multicopy replicative vector pREPにクローニング。
これをA. pretiosumへ戻して恒常的過剰産生させ、ansamitocin産生量への影響を見ている。

H. avellaneaへの感受性に基づいたansamitocin bioassayでの抑制指数は、integrative vectorでのasm34過剰発現で1.1倍の控えめな増加を示した。replicative vectorでは有意差なし。

close this sectionSequence

selected fasta
>putative TetR family transcriptional regulator [transcriptional regulator]
MARRNPERRAALLDAAVEVLAGEGARGLTFRAVDQRAGVPAGTASNYFAGRAEILTGAGE
RVYQRLLDQAPLGETPDGPRDRSRVTELMHALVDRVSAFPTGFLALLELRLEAARNPELR
EVLTARVRADLRFNAEHHAASGLPGDGTTVALLWLALNWLILERLTLPDLLTDHQRRDLV
TALVDRLLAGQPEEAGSPFDESGK
selected fasta
>putative TetR family transcriptional regulator [transcriptional regulator]
ATGGCGCGACGCAACCCGGAGCGGCGGGCCGCGCTGCTCGACGCCGCCGTCGAGGTGCTG
GCGGGCGAGGGCGCGCGGGGGCTCACCTTCCGGGCGGTCGACCAGCGGGCCGGGGTGCCC
GCGGGCACCGCGTCGAACTACTTCGCCGGGCGCGCCGAGATCCTCACCGGCGCGGGCGAG
CGGGTCTACCAGCGGCTGCTCGACCAGGCCCCGCTGGGCGAGACCCCGGACGGCCCGCGC
GACCGGTCCCGCGTCACCGAATTGATGCACGCCCTCGTCGACCGCGTCTCGGCCTTCCCG
ACCGGTTTCCTCGCCCTGCTGGAACTGCGCCTGGAGGCCGCGCGCAACCCGGAGCTGCGC
GAGGTCCTGACCGCCCGCGTCCGCGCCGACCTGCGCTTCAACGCCGAGCACCACGCCGCC
TCCGGCCTGCCCGGCGACGGCACCACCGTGGCGCTGCTGTGGCTGGCCCTCAACTGGCTG
ATCCTGGAACGCCTCACCCTCCCCGACCTGCTGACCGACCACCAGCGCCGCGACCTCGTC
ACCGCACTGGTCGACCGGCTGCTCGCGGGGCAGCCGGAGGAGGCGGGCAGCCCGTTCGAC
GAGAGCGGAAAATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001647 DNA-binding HTH domain, TetR-type (Domain)
[6-66] PS50977
PS50977   HTH_TETR_2
[12-55] 2.2e-07 PF00440
PF00440   TetR_N
IPR009057 Homeodomain-like (Domain)
[2-70] 2.79999889404114e-12 SSF46689
SSF46689   Homeodomain_like
IPR015893 Tetracycline transcriptional regulator, TetR-like, C-terminal (Domain)
[7-190] 4.2e-30 G3DSA:1.10.357.10
G3DSA:1.10.357.10   TetR_C
SignalP
[1-27] 0.192 Signal
Eukaryota   
TMHMM No significant hit
Ansam_00370 Ansam_00370   Ansam_00390 Ansam_00390
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