Mith_00110 : CDS information

Location

Organism	Streptomyces argillaceus
Strain	ATCC 12956
Entry name	Mithramycin
Contig	X89899
Start / Stop / Direction	14,873 / 13,329 / - [in whole cluster] 14,873 / 13,329 / - [in contig]
Location	complement(13329..14873) [in whole cluster] complement(13329..14873) [in contig]
Type	CDS
Length	1,545 bp (514 aa)

Annotation

Category	5.1 general function
Product	putative ligase
Product (GenBank)	acyl CoA ligase
Gene
Gene (GenBank)	mtmL
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q194Q9 PmId [10102355] Analysis of two chromosomal regions adjacent to genes for a type II polyketide synthase involved in the biosynthesis of the antitumor polyketide mithramycin in Streptomyces argillaceus. (Mol Gen Genet. , 1999) comment mithramycin PKSの上流・下流に位置する2つの染色体領域のクローニング、シークエンスから、8genesを同定。 mtmLはPKSの上流領域に含まれる。 CoA誘導体として有機酸や長鎖FAを活性化するacyl CoA ligasesに似ている。 acetyl-CoAは芳香族polyketidesの前駆体であり、ふつうは一次代謝によって生成されるが、mithramycin生産株はmithramycin経路にacetateを向かわせる効率的なシステムを持ち、そこでMtmLはacetate loading enzymeとして使用されるかもしれない。配列解析のみ。
Related Reference	ACC P41636 PmId [9008388] Molecular cloning of 4-coumarate:coenzyme A ligase in loblolly pine and the roles of this enzyme in the biosynthesis of lignin in compression wood. (Plant Physiol. , 1997) comment Blast 259th, id32%, 7e-45 Pinus taeda(テーダマツ)_4CL 4-coumarate--CoA ligase(EC 6.2.1.12) 圧縮ストレスにより木部での発現変化する 4CL activity with 4-coumaric acid: 上昇 with ferulic acid: 変化なし with caffeic acid: 低下

Sequence

>putative ligase [acyl CoA ligase]
MSELPPGPAVPLDGLLRLAAERHPEQVAVRDERGAVDYAGLDARADRFARALLRLTGGRP
TAVGVASVLDPVFAAAFYGTSRSGNRVVLVNPLVREPVLEHVFRTAGIEIALVSAETRSP
GGRRAALPDLREVYVVDADRTGPPPPGTRPLDDLLTEDGTAALPDPAGVDLDSVVCVQFT
SGTTGPPKGVRLTHRNLVANAAQAAHALGLDAGSVCLNHLPLYHVMHLDSAVYAGATQVL
CHDPDPVASVAAAAEAGATHYFGLPVRLARLAADPRLASVVPGPELGLVRSGGSRLAPAV
AARLRERLGVPVIQGYGLAELSPLSHNDRPERSKPGSVGPAVPGTECRIVDLETGAALDP
GRPGEVLLRGPQLMAGYLGLPDAPDIDAAGWFHTGDVGYQDEDGWLFLVDRIKDVFKVDN
ELVSPSEIEQVLLQDPDVADCVVADLPDEFSGAVVWAGVVPAGDGPVDLNPIVARANALL
SDHQRIRRAERLTAVPRSPNGKTERTRLRERLRA

>putative ligase [acyl CoA ligase]
GTGTCTGAGCTCCCGCCCGGGCCGGCGGTACCGCTGGACGGGTTGCTGCGCCTGGCCGCC
GAGCGGCACCCGGAGCAGGTCGCCGTGCGCGACGAGCGCGGAGCCGTCGACTACGCCGGA
CTCGACGCCCGCGCCGACCGGTTCGCCCGCGCCCTGCTGCGGCTGACCGGCGGCCGGCCG
ACGGCCGTCGGCGTGGCGAGCGTGCTGGACCCGGTGTTCGCGGCGGCCTTCTACGGCACC
AGCCGCAGCGGCAACCGCGTCGTGCTGGTCAACCCGCTGGTGCGCGAACCCGTCCTGGAG
CACGTCTTCCGCACGGCCGGCATCGAGATCGCGCTGGTCTCCGCCGAGACCCGGTCCCCT
GGCGGCCGTCGCGCCGCTCTCCCCGACCTGCGGGAGGTCTACGTGGTCGACGCGGACCGG
ACCGGGCCGCCGCCCCCGGGAACCCGCCCCCTGGACGACCTCCTCACCGAGGACGGCACG
GCCGCGCTGCCCGACCCGGCCGGTGTCGACCTGGACTCCGTCGTCTGCGTGCAGTTCACC
AGTGGCACCACCGGTCCCCCGAAGGGCGTCCGGCTCACCCACCGCAACCTGGTGGCCAAC
GCCGCCCAGGCGGCACACGCACTCGGCCTCGACGCCGGCAGCGTCTGCCTCAACCACCTG
CCGCTCTACCACGTCATGCACCTCGACTCGGCCGTGTACGCGGGCGCCACCCAGGTGCTG
TGCCACGACCCCGACCCGGTCGCCTCGGTCGCCGCGGCGGCCGAGGCCGGAGCCACCCAC
TACTTCGGGTTGCCGGTACGGCTGGCCAGACTGGCGGCGGACCCGCGGCTGGCCTCGGTC
GTCCCCGGGCCCGAACTGGGGCTGGTGCGCTCCGGCGGCTCCCGGCTCGCCCCCGCCGTC
GCGGCCCGCCTGCGCGAGCGGCTCGGCGTGCCGGTCATCCAGGGATACGGGCTCGCCGAA
CTCTCGCCGCTCAGCCACAACGACCGTCCGGAGCGGAGCAAGCCCGGCTCGGTCGGTCCG
GCCGTCCCCGGCACCGAGTGCCGCATCGTGGACCTGGAGACCGGAGCCGCCCTTGACCCC
GGCCGGCCGGGCGAGGTCCTGCTGCGCGGACCGCAGCTGATGGCCGGGTACCTCGGCCTG
CCGGACGCCCCGGACATCGACGCCGCAGGCTGGTTCCATACCGGCGACGTCGGCTACCAG
GACGAGGACGGCTGGCTGTTCCTGGTCGACCGCATCAAGGACGTCTTCAAGGTCGACAAC
GAACTCGTGTCGCCGAGCGAGATCGAACAGGTCCTGCTGCAGGACCCGGACGTCGCCGAC
TGTGTGGTGGCCGACCTGCCCGACGAGTTCAGCGGCGCCGTCGTCTGGGCCGGCGTCGTA
CCCGCCGGTGACGGGCCGGTGGACCTGAACCCGATCGTGGCCCGGGCCAACGCCCTGCTC
TCCGACCACCAGCGCATCCGCCGCGCCGAACGGCTCACGGCCGTGCCCCGCTCCCCGAAC
GGCAAGACCGAACGAACGCGGCTGCGCGAGCGGCTGCGCGCCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding IPR020845 AMP-binding, conserved site (Conserved_site) PS00455 AMP_BINDING
SignalP	No significant hit
TMHMM	No significant hit

Mith_00100 Mith_00120