Oxtet_00010 : CDS information

Location

Organism	Streptomyces rimosus
Strain	ATCC 10970 (=NBRC 12907)
Entry name	Oxytetracycline
Contig	DQ143963
Start / Stop / Direction	134 / 625 / + [in whole cluster] 134 / 625 / + [in contig]
Location	134..625 [in whole cluster] 134..625 [in contig]
Type	CDS
Length	492 bp (163 aa)

Annotation

Category	4.1 transcriptional regulator
Product	MarR family transcriptional repressor
Product (GenBank)	OxyTA1
Gene	oxyTA1 otrR
Gene (GenBank)	oxyTA1
EC number
Keyword
Note
Note (GenBank)	putative transcription regulation
Reference	ACC Q3S8R5 PmId [16597959] Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase. (Appl Environ Microbiol. , 2006) [27100123] Development of a Synthetic Oxytetracycline-Inducible Expression System for Streptomycetes Using de Novo Characterized Genetic Parts. (ACS Synth Biol. , 2016) comment [PMID: 16597959](2006) oxytetracycline gene clusterのシークエンス、配列解析。 resistance genes otrAとotrBの間に全21genesを同定。わかりやすくするため、gene namesがotc → oxyに変更され、振り直されている。 oxyTA1 (otrR): Transcription regulation 詳細記載なし。 --- [PMID: 27100123](2016) Streptomyces rimosus由来 oxytetracycline responsive repressor OtrR、operator otrO、promoter otrBpに基づいたoxytetracycline誘導性発現システムの開発。
Family/Domain	FD IPR000835 comment [IPR000835] Transcription regulator HTH, MarR-type (Family) function; sequence-specific DNA binding transcription factor activity

Sequence

>MarR family transcriptional repressor [OxyTA1]
MDSSAPDLAALIEVTAEVFAVNGRLLREGDSLTAHAGLTSARWQVAGLLLSGPSTVARLA
RERGLRRQAVQQTVERLKAEGVVTTRPNPQDQRSPLVELTARGRQALDDLRPLERRWLEY
LAEDIPVEDMRVAIAVLSRLREKLDARPATEFGTGAGSGRQSA

>MarR family transcriptional repressor [OxyTA1]
ATGGATTCCTCAGCCCCTGACCTGGCCGCTCTGATCGAGGTGACCGCCGAGGTCTTCGCG
GTCAACGGCCGCCTGCTCCGCGAAGGCGACAGCCTCACCGCCCACGCGGGGCTGACCTCG
GCGCGCTGGCAGGTGGCCGGACTGCTGCTGAGCGGCCCCTCGACGGTCGCCCGCCTGGCC
CGCGAGCGGGGGCTGCGGCGGCAGGCGGTCCAGCAGACCGTCGAGCGGCTGAAGGCCGAG
GGCGTCGTCACGACCCGGCCCAACCCGCAGGACCAGCGCAGCCCCCTGGTCGAGCTCACC
GCACGCGGCCGGCAGGCGCTGGACGACCTGCGTCCCCTGGAACGGCGGTGGCTGGAGTAT
CTGGCCGAGGACATTCCGGTCGAGGACATGCGCGTGGCGATCGCGGTGCTGAGCCGCCTG
CGGGAGAAGCTGGACGCCCGTCCGGCGACGGAGTTCGGGACCGGGGCCGGGTCCGGGCGG
CAGTCCGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000835 Transcription regulator HTH, MarR-type (Family) PS50995 HTH_MARR_2 IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd
SignalP	No significant hit
TMHMM	No significant hit

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