C1027_00120 C1027_00120   C1027_00140 C1027_00140

C1027_00130 : CDS information

close this sectionLocation

Organism
StrainC-1027
Entry nameC-1027
Contig
Start / Stop / Direction16,653 / 15,919 / - [in whole cluster]
16,653 / 15,919 / - [in contig]
Locationcomplement(15919..16653) [in whole cluster]
complement(15919..16653) [in contig]
TypeCDS
Length735 bp (244 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose N,N-dimethyltransferase
Product (GenBank)N-methyl transferase
GenesgcA5
Gene (GenBank)
EC number2.1.1.-
Keyword
  • deoxy aminosugar
Note
Note (GenBank)
  • ORF7
Reference
ACC
PmId
[12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002)
comment
C-1027の生合成遺伝子クラスターの報告

sgcA to sgcA6, encoding deoxyaminosugar biosynthesis.
機能解析はしていない。
N-methyl transferase(SgcA5)としている
Related Reference
ACC
Q9L4U1
NITE
Acla_00260
PmId
[11016846] A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin. (Mol Gen Genet. , 2000)
[12427929] Characterization of mutations in aclacinomycin A-non-producing Streptomyces galilaeus strains with altered glycosylation patterns. (Microbiology. , 2002)
comment
Streptomyces galilaeus_aknX2
Putative aminomethylase

[PMID: 11016846]
anthracycline生合成gene clusterの15kb領域をクローニング、特徴づけ。

AknX: Aminomethylase

AknXはN-methyltransferasesに約60%の類似を示す。
AknX, Y, Zは糖残基の生合成に関連付けられる。

glycosyltransferase (aknS), aminomethylase (aknX), glucose-1-phosphate thymidylyltransferase (aknY) とunidentified glycosylation functions (aknT and aknV)を含むplasmid pSgs4は、aklavinoneを蓄積するS. galilaeus mutants H063と、rhodinose and deoxyfucose residuesを持つaklavinoneを産生するH054でaclacinomycinsの産生を回復。

ε-rhodomycinone(RHO)を蓄積するS. peucetius mutants M18におけるpSgs4の導入は、
RHO, L-rhamnosyl-RHO, L-daunosaminyl-RHO をもたらす。
glycosyltransferasesに欠損がないRHO蓄積S. peucetius mutants M90へのpSgs4の導入は、
RHO と L-rhamnosyl-RHO をもたらす。

また、aknW, aknX2, aknYとC末を欠いたaknZを含むpSgs44は、H054とH065 mutantを部分的に相補する。

--
[PMID: 12427929]
同じ著者らの続報。
上記論文を引用してFig. 2b(Sg9 gene cluster organization)とTable 4を記載。

Table 4. Putative functions of Sg9 gene products
AknX2: aminomethylase
よってここでのAknX2=AknX

上記論文の結果を受けて行った実験で、mutantの表現型はAknX2に由来しないことを確認している。
ACC
Q9ALP0
NITE
Spino_00030
PmId
[11358695] Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. (Chem Biol. , 2001)
[11386361] A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa. (Antonie Van Leeuwenhoek. , 2000)
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
Saccharopolyspora spinosa
Probable N-dimethyltransferase

[PMID:11358695]
spinosad biosynthetic gene clusterの報告。
spnS(250aa): forosamine; dimethyltransferase

--
[PMID:11386361]
同じ著者らの先行論文。
spaS(spnS?): Forosamine synthesis

--
[PMID: 18345667]
D-forosamine生合成に関連するspnO, N, Q, R, and Sのクローニング、特徴づけ。
使用している株はNRRL 18537.

dTDP-D-forosamine生合成での最終ステップを触媒するN,N-dimethyltransferaseとしてのSpnSの機能を確立するため、SpnSをE.coliで発現、精製、タンパク確認、酵素活性確認。
十分な量の予測基質を用意できないため、phosphonate mimicを基質としてmonomethylated, dimethylated産物が得られることを確認。

close this sectionSequence

selected fasta
>putative NDP-hexose N,N-dimethyltransferase [N-methyl transferase]
MEYGPEHAKFYDLVFRSRGKDFDLEARGLTELILAARPDAVSLLDVACGTGAHLETLATL
FGHVEGLEYAPAMLEQAAGRLPGVPLHAGDMRSFDLGRTFDAITCMGNALGEMGSVTELK
AAVSAMAHHLNPGGVLVAEPWYFPENFLDGHVGGHLHQEEGRVITRMTHSVRQGDKSRLE
VRFRVADASGFREFSEVLTSSLFTREQYTDAFESVGLSVRFVPGFRLADGRPNSPGLFVG
VRTA
selected fasta
>putative NDP-hexose N,N-dimethyltransferase [N-methyl transferase]
ATGGAGTACGGCCCCGAGCACGCGAAGTTCTACGACCTCGTGTTCCGCAGCCGCGGCAAG
GACTTCGACCTGGAGGCCCGCGGGCTCACCGAGCTGATCCTCGCCGCCCGCCCCGACGCC
GTGTCGCTCCTGGACGTCGCCTGCGGCACCGGAGCCCATCTGGAGACGCTCGCCACGCTC
TTCGGCCATGTGGAGGGCCTCGAGTACGCGCCCGCGATGCTGGAGCAGGCGGCGGGCCGG
CTTCCCGGCGTCCCGCTGCACGCCGGTGACATGCGTTCGTTCGACCTGGGCCGCACCTTC
GACGCGATCACCTGCATGGGCAACGCGCTCGGCGAGATGGGGTCCGTCACCGAGCTCAAG
GCCGCGGTGTCGGCCATGGCCCACCACCTGAACCCCGGGGGAGTCCTCGTCGCCGAACCC
TGGTATTTCCCCGAGAACTTCCTCGACGGGCATGTCGGCGGCCATCTGCACCAGGAGGAG
GGCCGTGTCATCACGCGGATGACGCACTCCGTGCGCCAGGGCGACAAGTCACGTCTGGAG
GTCCGGTTCCGGGTCGCGGACGCCTCCGGCTTCAGAGAGTTCTCCGAGGTCCTGACCTCC
AGCCTCTTCACCCGCGAGCAGTACACCGACGCCTTCGAATCGGTGGGGCTCTCGGTGCGG
TTCGTGCCCGGCTTCCGGCTGGCCGACGGCCGGCCCAACAGCCCCGGCCTCTTCGTCGGC
GTGCGCACGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
C1027_00120 C1027_00120   C1027_00140 C1027_00140
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