C1027_00415 C1027_00415   C1027_00430 C1027_00430

C1027_00420 : CDS information

close this sectionLocation

Organism
StrainC-1027
Entry nameC-1027
Contig
Start / Stop / Direction54,226 / 55,374 / + [in whole cluster]
54,226 / 55,374 / + [in contig]
Location54226..55374 [in whole cluster]
54226..55374 [in contig]
TypeCDS
Length1,149 bp (382 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Producttranscriptional repressor
Product (GenBank)unknown
GenesgcR
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF34
Reference
ACC
PmId
[12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002)
[21250756] Improvement of the enediyne antitumor antibiotic C-1027 production by manipulating its biosynthetic pathway regulation in Streptomyces globisporus. (J Nat Prod. , 2011)
comment
[PMID:1218628]
C-1027の生合成遺伝子クラスターの報告

Fig.2にてcluster内に表示されているのみで、特に記述なし。


[PMID:21250756]
SgcR, SgcEの機能解析

putative DNA-binding proteinsと推定されるsgcE1,sgcRの不活性化株を作製。sgcE1はC-1027の生合成に影響を与えなかったがsgcR不活性化株はC-1027とheptaeneのtitersが著しく増加した。したがって、SgcRはC-1027のnegative regulatorであることを示した。また、sgcR欠損株においてsgcR1の過剰発現させると、C-1027産生は約7倍に上がった。

SgcRは、Streptomycetes genomesの配列の中で2つの機能未知タンパクと唯一相同性を示すことから、regulatory proteinsのuncharacterized familyに属するかもしれないと推測している。

close this sectionSequence

selected fasta
>transcriptional repressor [unknown]
MSTGYLSRLESGARQPSDRAVAHLAGQLGISPSEFEGSRATSLAQILSLSTSLESDETSE
LLAEAVRSAHGQDPMLRWQALWLLGQWKRRHGDSAGEHGYLQRLVTLSEEIGLAELRARA
LTQFARSLRVLGEIVPAVEAAAAAHRLAVDHALSSQDRAASLLVLVSVEAEAGRMPDARR
HADELTVLVRGRSDTLWAEALWTAGALKVRQGEFAAAEVLFQEALDGFDSRENLTIWLRL
RIAMAELHLQKLPPEPDAAQLCIEAAEAALPFARTSALEQSLAALRARLAFHEGRFADAR
ALLERLGRTELRLPYQSRIRLEVLGHQLRILSGEEEEGLAGLQLLAEEAQENSNINLAAE
IWRLAAECLMRARGKVRGATGG
selected fasta
>transcriptional repressor [unknown]
ATGTCTACGGGCTATCTCTCGCGCCTGGAGTCGGGCGCCCGGCAGCCCTCCGATCGCGCC
GTCGCCCACCTGGCCGGACAACTCGGCATCAGCCCGTCGGAGTTCGAAGGGTCCCGGGCC
ACCTCGCTCGCCCAGATCCTCTCCCTCTCCACTTCCCTGGAGTCCGACGAGACCAGTGAG
CTTCTCGCCGAGGCGGTACGTTCCGCGCATGGCCAGGATCCGATGCTCCGCTGGCAGGCC
CTGTGGCTGCTGGGACAGTGGAAGCGCCGGCACGGCGACTCGGCCGGCGAGCACGGCTAC
CTCCAGCGTCTGGTGACGCTGAGTGAGGAGATCGGCCTGGCCGAGTTGCGCGCACGGGCC
CTGACCCAGTTCGCCCGGTCGCTGCGGGTACTGGGCGAGATCGTTCCGGCGGTGGAGGCT
GCCGCCGCCGCCCACCGGCTCGCGGTGGACCATGCGCTGTCCAGCCAGGACAGGGCCGCT
TCGCTGCTGGTTCTGGTGTCGGTGGAGGCCGAGGCGGGACGGATGCCCGACGCCCGGCGC
CACGCCGACGAACTGACCGTCCTGGTGAGGGGACGGTCCGACACTCTGTGGGCCGAGGCG
TTGTGGACGGCGGGTGCGTTGAAGGTGCGGCAGGGCGAGTTCGCCGCGGCCGAGGTCCTT
TTCCAGGAGGCTCTGGACGGGTTCGACAGCCGGGAGAACCTGACGATCTGGCTGCGGCTG
CGCATCGCGATGGCCGAACTCCACCTGCAGAAACTTCCTCCCGAGCCCGACGCCGCGCAG
CTCTGCATCGAGGCGGCGGAGGCGGCCCTTCCCTTTGCCCGCACATCCGCTCTGGAACAG
TCCCTCGCCGCTCTGCGGGCGCGCCTCGCCTTCCATGAGGGCAGGTTCGCCGATGCCCGC
GCGTTGTTGGAGAGGCTCGGCAGGACCGAGCTCCGGCTGCCCTATCAGAGCCGGATCCGC
CTGGAGGTCCTCGGTCATCAGCTGCGCATCCTGAGCGGGGAGGAGGAGGAAGGCCTGGCC
GGCCTCCAGCTCCTGGCCGAGGAGGCGCAGGAGAACTCCAACATCAACCTCGCCGCGGAG
ATCTGGCGGCTCGCGGCGGAATGCCTGATGCGGGCGCGCGGGAAGGTCCGCGGCGCCACC
GGCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR010982 Lambda repressor-like, DNA-binding domain (Domain)
[1-38] 0.00041 G3DSA:1.10.260.40
G3DSA:1.10.260.40   G3DSA:1.10.260.40
IPR011990 Tetratricopeptide-like helical (Domain)
[54-351] 8.60000000000001e-10 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
SignalP No significant hit
TMHMM No significant hit
C1027_00415 C1027_00415   C1027_00430 C1027_00430
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