C1027_00460 : CDS information

Location

Organism	Streptomyces globisporus
Strain	C-1027
Entry name	C-1027
Contig	AY048670
Start / Stop / Direction	60,090 / 58,435 / - [in whole cluster] 60,090 / 58,435 / - [in contig]
Location	complement(58435..60090) [in whole cluster] complement(58435..60090) [in contig]
Type	CDS
Length	1,656 bp (551 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	oxidoreductase
Gene	sgcE9
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF38
Reference	ACC Q8GME7 PmId [12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002) comment C-1027の生合成遺伝子クラスターの報告 SgcE6, SgcE7, SgcE9 は様々なoxidoreductaseに似ていると記述有り。
Related Reference	ACC P12676 PmId [2914858] Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp. (J Bacteriol. , 1989) [10194345] Crystal structure determination of cholesterol oxidase from Streptomyces and structural characterization of key active site mutants. (Biochemistry. , 1999) comment Streptomyces sp._choA Cholesterol oxidase EC 1.1.3.6/5.3.3.1 [24%id, 2e-13] [PMID:2914858] choA geneのシーケンス解析。 secreted cholesterol oxidaseであると推定された。 [PMID:10194345] choAの結晶構造解析活性中心が同定されている。このORF(C1027_00460)ではE361がD、H447がHになっており一部保存されていない。
Family/Domain	FD IPR000172, IPR007867 comment [IPR000172] Glucose-methanol-choline oxidoreductase, N-terminal (Domain) [IPR007867] Glucose-methanol-choline oxidoreductase, C-terminal (Domain)

Sequence

>putative oxidoreductase [oxidoreductase]
MSTTAERTDVLVIGSGFGGAIAAYHLAAGGADVTVLERGPWLESKEFEHDYKLGSSYTRA
FDFTVGDGMSILGGNCVGGGSVVYFAAMPRAPRFVFDRQGSIGRRMWPQAVSRETLDPWY
DRVEESLSVTRQDWNDVSYAGGLWAAACNHAGRTANPLAVAIDNTKCVNCNWMMAGCRFE
AKQSLLVNYLPAAIAHGARIRPLHEVQHLSRTPDGSYRVHYNVVHDDDYRLQAGSGVIEA
KIVVMAAGAGATPVILQRSEAHLGTMPRAVGRYFSGNGERLNTAIIDEAKAAELFGLDRG
DGLAYAANQIGKGPTVASWDRLDGSLPEYSRYSLEQLYFPPGLGTILAQVPGATGPSWFG
KEKKEILKQWTSWLTIFTMIEDDNEGVFGPPPATGNAHRISQQMLGRGNLRYDPTKNTLG
AWAASDAEVKEILEKDGLAKVMPWTNDLVGAYTVHPLSSCRMGDDPHTSALDDSNELRDH
PGIFVTDGSSVPGALTVNPAMTIAALAERAMPGIVRAAQSRGISVTYGAPAPDGSTSGRE
RVLPLLPSARG

>putative oxidoreductase [oxidoreductase]
ATGAGCACCACGGCCGAGCGCACGGACGTCCTCGTCATCGGCAGCGGATTCGGCGGTGCC
ATCGCCGCCTACCACCTGGCGGCGGGGGGCGCCGACGTCACCGTCCTGGAACGCGGGCCG
TGGCTGGAGAGCAAGGAGTTCGAGCACGACTACAAACTCGGCTCCTCGTACACGAGGGCG
TTCGACTTCACGGTCGGGGACGGCATGAGCATCCTCGGCGGGAACTGTGTGGGCGGCGGC
AGCGTCGTCTACTTCGCCGCGATGCCCCGCGCCCCGCGCTTCGTCTTCGACCGGCAGGGG
TCCATAGGACGCCGGATGTGGCCGCAGGCGGTGAGCCGCGAAACCCTGGACCCCTGGTAC
GACCGTGTCGAGGAGTCCCTCTCCGTGACCCGGCAGGACTGGAACGACGTGAGCTACGCC
GGCGGACTGTGGGCCGCGGCGTGCAACCACGCCGGCCGCACCGCCAACCCCCTCGCCGTC
GCCATCGACAACACCAAGTGCGTGAACTGCAACTGGATGATGGCCGGCTGCCGCTTCGAG
GCCAAGCAGTCCTTGCTGGTCAACTACCTGCCCGCCGCCATCGCCCATGGGGCGCGCATC
CGCCCGCTGCACGAGGTCCAGCACCTCTCGCGCACCCCCGACGGCTCCTACCGGGTGCAC
TACAACGTCGTGCACGACGACGACTACCGCCTCCAGGCGGGCAGCGGCGTCATCGAGGCG
AAGATCGTGGTGATGGCGGCCGGCGCGGGCGCGACCCCGGTGATCCTCCAGCGCAGCGAG
GCCCACCTGGGCACCATGCCACGAGCGGTCGGACGCTACTTCTCCGGCAACGGCGAGCGG
CTCAACACCGCCATCATCGACGAGGCCAAGGCGGCCGAGCTGTTCGGCCTGGACCGGGGC
GACGGGCTCGCCTACGCGGCCAACCAGATCGGCAAGGGGCCCACCGTGGCGAGCTGGGAC
AGGCTGGACGGCTCGTTGCCCGAGTACTCCCGCTACTCCCTGGAACAGCTCTACTTCCCG
CCGGGCCTCGGCACGATCCTCGCCCAGGTGCCCGGCGCCACGGGCCCCTCCTGGTTCGGC
AAGGAGAAGAAGGAGATCCTGAAGCAGTGGACCTCCTGGCTCACGATCTTCACGATGATC
GAGGACGACAACGAAGGCGTCTTCGGCCCCCCTCCCGCCACCGGTAACGCCCACCGCATC
TCCCAGCAGATGCTCGGCCGGGGCAACCTGCGCTACGACCCGACGAAGAACACCCTGGGC
GCCTGGGCCGCATCCGACGCCGAGGTCAAGGAGATCCTGGAGAAGGACGGCCTGGCCAAG
GTCATGCCCTGGACCAACGACCTCGTCGGCGCCTACACCGTCCACCCGCTCTCCTCCTGC
CGGATGGGCGACGACCCCCACACATCCGCCCTGGACGACAGCAACGAGCTCCGCGACCAC
CCGGGGATCTTCGTCACCGACGGATCGTCGGTTCCCGGCGCGCTGACGGTCAATCCCGCG
ATGACCATCGCGGCGCTCGCCGAACGCGCGATGCCGGGGATCGTGCGCGCCGCGCAGAGC
CGGGGTATCTCCGTCACCTACGGCGCACCGGCCCCCGACGGCTCCACGAGCGGCCGCGAG
AGGGTTCTTCCCCTGCTGCCGTCGGCGCGCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000172 Glucose-methanol-choline oxidoreductase, N-terminal (Domain) PF00732 GMC_oxred_N IPR007867 Glucose-methanol-choline oxidoreductase, C-terminal (Domain) PF05199 GMC_oxred_C
SignalP	No significant hit
TMHMM	No significant hit

C1027_00450 C1027_00470