C1027_00590 C1027_00590   C1027_00610 C1027_00610

C1027_00600 : CDS information

close this sectionLocation

Organism
StrainC-1027
Entry nameC-1027
Contig
Start / Stop / Direction78,769 / 77,582 / - [in whole cluster]
78,769 / 77,582 / - [in contig]
Locationcomplement(77582..78769) [in whole cluster]
complement(77582..78769) [in contig]
TypeCDS
Length1,188 bp (395 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Producttranscriptional activator
Product (GenBank)TylR regulatory protein-like protein
GenesgcR3
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF54
Reference
ACC
PmId
[12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002)
[19159491] Role of sgcR3 in positive regulation of enediyne antibiotic C-1027 production of Streptomyces globisporus C-1027. (BMC Microbiol. , 2009)
comment
[PMID:12183628]
C-1027の生合成遺伝子クラスターの報告

ORF54=sgcR3
genebank ID ORF55(=C1027_00610(論文中ではORF54としている))を不活性化するとC-1027の生合成に影響はないことから、sgcR3までがC-1027生合成clusterだとしている。


[PMID:19159491]
C-1027の生合成遺伝子で制御遺伝子としてSgcR1,R2,R3を同定。そのうちのSgcR3に関しての機能解析論文。

sgcR3過剰発現株はC-1027の産生能が上がり、sgcR3欠損株はC-1027が産生されなかった。
また、sgcR3欠損株の転写解析ではsgcA1やsgcC4のような生合成構造遺伝子だけでなく、制御遺伝子sgcR1,sgcR2の転写量も減少した。 cross-complementation studyにて、sgcR3がsgcR1R2のプロモーター領域に直接結合することでsgcR1とsgcR2の転写を活性化した。

close this sectionSequence

selected fasta
>transcriptional activator [TylR regulatory protein-like protein]
MAAHDVRHAHLPVSVLADQIFGHLPRADQRAWAQAYLTGLLTTDGKKSIRRMAATVSRSP
TASQSMHQFLNASPWDWAPARAELMRWVEQQLDPRAWVLSVAVLRKRGDHSCGVHRRFVP
ATGRSVNCQLAVGAFLSTGATAVPVHWGLLLPGAWVEDHARRARARIPESVGQRSVEQHA
LDLVDALCGSTQLSPRPVVADLSHHSGVISLVRGLTARGREFVVSLPGRTPVVPVGRTTA
RGAYSANLPAPVEAQRLFGLKHAGHLRPGAHDGHGGADRTATMTTLVRLPQVRLARHTPH
ATYRIFGMPSYSGPRSARIWLTNMTQRRTDELLALAGLQHRAGTAVQSLEDDFGLLDFEG
RSFPGWHHHMTLVSAASAYNLLELSGRRPGSQEGS
selected fasta
>transcriptional activator [TylR regulatory protein-like protein]
ATGGCGGCACACGACGTGAGGCACGCGCACCTGCCGGTCTCCGTACTTGCCGATCAGATC
TTCGGGCACCTGCCGAGGGCCGATCAGAGGGCGTGGGCCCAGGCCTACCTGACGGGGCTG
CTGACCACCGACGGCAAGAAGTCGATCAGACGGATGGCCGCCACCGTCTCCCGTTCGCCC
ACCGCGTCGCAGTCGATGCACCAGTTCCTCAATGCCAGCCCCTGGGACTGGGCCCCGGCA
CGTGCCGAGCTGATGAGATGGGTCGAGCAGCAGCTCGATCCGCGCGCCTGGGTCCTGTCG
GTGGCGGTGTTACGCAAGCGGGGAGATCACTCCTGCGGGGTGCACCGGCGCTTCGTCCCC
GCCACCGGACGCTCCGTCAACTGCCAGCTCGCGGTGGGTGCGTTCCTCTCCACCGGCGCG
ACGGCCGTGCCCGTCCACTGGGGGCTCCTGCTGCCCGGGGCCTGGGTCGAGGACCATGCG
CGCCGCGCACGGGCCCGCATCCCCGAATCGGTCGGCCAGCGCAGCGTCGAGCAGCATGCG
CTGGACCTCGTCGACGCGCTGTGCGGGAGCACGCAACTGAGTCCCCGGCCGGTGGTGGCC
GATCTGAGTCATCATTCCGGGGTCATCTCGCTGGTGCGCGGACTCACCGCCAGGGGACGC
GAGTTCGTCGTGTCACTGCCGGGCCGGACCCCGGTGGTCCCGGTGGGGAGAACGACGGCG
CGCGGCGCGTACTCCGCGAACCTGCCCGCCCCCGTGGAGGCACAGCGCCTCTTCGGCCTC
AAGCACGCCGGGCATCTCAGACCCGGCGCCCATGATGGGCACGGCGGCGCCGATCGCACC
GCCACGATGACCACGCTGGTGCGGCTGCCGCAGGTGCGGCTGGCACGTCACACGCCGCAC
GCCACGTACCGGATCTTCGGTATGCCGTCCTACTCGGGGCCCCGCTCCGCGCGGATCTGG
CTGACCAACATGACGCAGCGGCGGACCGACGAACTGCTGGCGCTGGCCGGGCTCCAGCAC
CGGGCGGGCACCGCGGTGCAGTCGCTGGAGGACGACTTCGGTCTGCTCGACTTCGAGGGA
CGGTCCTTTCCCGGCTGGCATCACCACATGACTCTCGTGTCGGCGGCTTCGGCGTACAAC
CTGCTGGAGCTGTCCGGCCGCCGGCCCGGCTCCCAGGAGGGGAGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
C1027_00590 C1027_00590   C1027_00610 C1027_00610
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