Teicp2_00200 Teicp2_00200   Teicp2_00220 Teicp2_00220

Teicp2_00210 : CDS information

close this sectionLocation

Organism
StrainATCC 31121 (=NBRC 13999)
Entry nameTeicoplanin
Contig
Start / Stop / Direction42,301 / 47,898 / + [in whole cluster]
42,301 / 47,898 / + [in contig]
Location42301..47898 [in whole cluster]
42301..47898 [in contig]
TypeCDS
Length5,598 bp (1,865 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.2 NRPS
Productnon-ribosomal peptide synthetase
Product (GenBank)non-ribosomal peptide synthetase
Gene
Gene (GenBank)tcp12
EC number
Keyword
Note
Note (GenBank)
  • contains Module 7(Condensation, Adenylation, Thiolation, unknown and Thioesterase domains)
Reference
ACC
PmId
[11386360] Teicoplanin biosynthesis genes in Actinoplanes teichomyceticus. (Antonie Van Leeuwenhoek. , 2000)
[14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004)
[25686610] X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. (Nature. , 2015)
[26549530] Sequential In Vitro Cyclization by Cytochrome P450 Enzymes of Glycopeptide Antibiotic Precursors Bearing the X-Domain from Nonribosomal Peptide Biosynthesis. (Angew Chem Int Ed Engl. , 2015)
[27213615] Regulation of the P450 Oxygenation Cascade Involved in Glycopeptide Antibiotic Biosynthesis. (J Am Chem Soc. , 2016)
comment
[PMID: 11386360, 14702401]
ATCC 31121株teicoplanin生合成gene clusterの同定論文。

tcp ORF12: NRPS, module 7

teicoplanin heptapeptide骨格を合成するNRPS4つのうち4つ目。
実験確認なし。

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[PMID: 25686610](2015)
SUPPLEMENTARY INFORMATION内のリストにこのUniProt entryの記載があるが、同グループの他論文(PMID: 25358800, 25586301)ではDSM43866株を使用してATCC 31121株由来のgene nameを採用している混乱があるため、同様の可能性あり。

X-domainの結晶構造解析や相互作用の実験から、X-domainがcytochrome P450(oxy genesでコードされる)による架橋形成に関与することがわかった。

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[PMID: 26549530](2015) abstract + supporting information
PMID: 25686610の続報。同様にsupporting informationでこのUniProt entryの記載あり。その他タンパクもATCC 31121株のentriesを採用している。
Related Reference
ACC
Q6ZZJ3
NITE
Teicp_00150
PmId
[15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004)
[25586301] Structure of the terminal PCP domain of the non-ribosomal peptide synthetase in teicoplanin biosynthesis. (Proteins. , 2015)
comment
[PMID: 15113000](2004)
DSM 43866株teicoplanin生合成gene clusterの同定論文。

teiD: Peptide synthetase; module 7

teicoplanin heptapeptide骨格を合成するNRPS4つのうち4つ目。
実験確認なし。

---
[PMID: 25586301](2015)
DSM(Z) 43866株を使用しているが、gene nameはATCC 31121株由来のtcpを採用している。

module 7のPCP domainの構造解析。
一般的な折りたたみを示す。

close this sectionPKS/NRPS Module

A7 L-3,5-dihydroxyphenylglycine(Dpg)
C15..310
A493..894
PCP976..1043
X1057..1356
TE1630..1850

close this sectionSequence

selected fasta
>non-ribosomal peptide synthetase [non-ribosomal peptide synthetase]
MTVDDTRAKPRSSVEDVWPLSPLQEGMLYHTALDGDGPDTYTVQTVYGIDGPLDAGRLRA
SWQALVDRHAALRACFRYVSGAQMVQVIQRDVELPWRETDLSGLPEDLADGEVARLAEQE
MAERLRLEAAPLMKLHLIRLGPQRHRLVHTLHHVLVDGWSMPILHRELAAIYAAGGDASG
LPPAVSYRDYLAWLGRQDKESARAAWRDALAGLDTPTTVAPADPARVPDIDTAMVELSPE
LTDDLLRLAHGHDLTLNTVVQGVWAVVLAQLAGRTDVVFGATASGRPAELAGVEAMVGLL
LATLPVRVRLDGRQRVVDMLADLQRDQSALRAHQHLGLQEVQAVVGPGAVFDTLVIYENF
PRTGLGGSADDGLVMRPVQKGRDSSHYPFTLVTGPGERMPVILYYDRGLFDQAAATSVLG
AIVRVLERLVAEPGVLVGRLTLLGEAERAVVVGDWNATAGPVPGDSMVELFDRRVAAAPD
TVAITDADGADLTYAEVDQASNRLAAHLADRGVRRGDRVGVAVERSADLPIVFLAIWKAG
AAYVPVDVGYPAERIALILAGSAVSTVICTQATEAAVPQNVPQRATIVLDAPPTRAAVDA
CAGTAPTFRPSAADLAYVMYTSGSTGVPKGVAVPHGAVAGLAGDAGWRIGPGDGVLMHAT
HVFDPSLYEMWVPLATGGRVLVAAPGVVDAGGIRQAVARGATAVHLTAGTFRALAEASPD
CFAGLREVGTGGDVVPAHTVAHLRRAQPQLRVRNTYGPTETTLCATWKPIEPGAQLGPEL
PIGRPMTNRRIYILDAFLRPVAPGVAGELYIAGTGLARGYLSRPDLTAERFVACPFLAGE
RMYRTGDLARWNRDGEVVFLGRADDQVKIRGYRVELAEVEAVLAAQPGVREAVVVAREDR
PGERRLVGYVVSDAGELDTEQIRQQMARVLPGYMVPVAVIGLVSLPITANSKVDRRALPA
PDLAGHASAKAPESATEKVLCALYAEILGVERVGVDDAFHDLGGSSALAMRLIARIREEL
GVDLPIRQLFSSPTPAGVARALAAKSRPALEAVTRPERVPLTARQLRAWLLARPSEETRG
RHLSVALRLRGRLDVAALEAALRDVAARHEILRTTFPGDAQTVHQHIHDAAPVRLTPVPA
TEEDLPARLAERGEQLFDLTRDMPWRCELFALSEKEHVLSVTVHRIAADDDSMDVFFRDL
AAAYGARRAGRAPERAPLALQFADYAIWEQRLLDGEREQDSLINDQITFWRNHLAGIDQE
TVLPFDRARPAIPSRRAGTVALRLDAGPHARLAEAVESAGADMPQLVQAALAMLLTRYGA
GTDLVIGTTLPRDEDLIDLEPMIGPFARPFPVRTDLSADPTFLEVVARVQEAVREARQHL
DVPFEKIPELLALPGSLSRHPVYQVGLQVREEDNGAWDAAELPALRTSVEPTGVEAIELD
LAFALTERRNDDDDEDGIEGALHYAADLFDHDTAASLARRLVRVLEQVAEDPGRRISDLD
ILLDDAERGAPAIAPARWAGAVPAAVADLVAAGPLGALVLDEAQRPVAPGTVGDLWVTGP
AVDADTGGRPSVPCPFGTPGHRMLHTGLLARKSPARTLVVVGERRGASASAKTGDYEVLL
PLRAAGDRAPLFCIHASGGLSWNYGPLLRHLPPNQPVYGVQARGLARTEPLPGSVEEMAA
DYVEQIRTVQPTGPYHLLGWSLGGRIAQAMAVLLEADGEKVGLLALLAAYPVYMGRKATG
TVTEEAARDEDALERRKQQELELAGQLVQGAGARSRLEAVMRNLWEVGPAHTASPFASDV
LLFVASVDRPAHLPVSEAIASWKDFTGGTIEPHEIATNHYEMVQPAALAQIGAIVAEKLR
SRPDA
selected fasta
>non-ribosomal peptide synthetase [non-ribosomal peptide synthetase]
GTGACCGTTGACGACACTCGTGCAAAGCCCCGCTCCAGCGTGGAAGACGTCTGGCCGCTG
TCGCCGCTGCAGGAGGGCATGCTTTACCACACCGCCCTGGACGGGGACGGGCCGGACACC
TACACCGTGCAGACCGTCTACGGCATCGACGGCCCGCTGGACGCCGGGCGGCTGAGAGCG
TCGTGGCAGGCCCTGGTGGACCGGCATGCCGCCCTGCGGGCCTGCTTCCGCTACGTCAGC
GGCGCGCAGATGGTGCAGGTCATTCAGCGGGACGTCGAGCTCCCGTGGCGGGAGACCGAC
CTGTCCGGGCTGCCGGAGGACCTCGCCGACGGTGAGGTGGCCCGCCTGGCGGAGCAGGAG
ATGGCCGAGCGCCTGCGCCTGGAGGCCGCGCCGCTGATGAAACTGCACCTGATCCGGCTC
GGCCCGCAGCGCCACCGGCTCGTGCACACCCTGCACCACGTGCTGGTGGACGGCTGGTCG
ATGCCGATCCTGCACCGTGAGCTCGCGGCGATCTACGCGGCGGGCGGGGACGCGTCCGGG
CTGCCGCCCGCGGTGTCGTACCGCGACTATCTGGCCTGGCTGGGCCGGCAGGACAAGGAG
TCGGCGCGGGCGGCGTGGCGGGACGCGCTCGCCGGGCTGGACACCCCGACCACGGTCGCG
CCGGCCGATCCGGCCCGCGTGCCGGACATCGACACGGCGATGGTGGAGCTGTCCCCCGAG
CTGACCGACGACCTGCTCCGGCTGGCGCATGGCCACGACCTCACGCTCAACACCGTCGTG
CAGGGCGTGTGGGCGGTCGTGCTGGCGCAGCTGGCCGGCCGTACCGACGTGGTGTTCGGC
GCGACCGCCTCGGGGCGGCCGGCCGAGCTGGCCGGGGTGGAGGCGATGGTCGGGCTGCTG
CTGGCCACCCTGCCGGTGCGGGTCCGGCTCGACGGGCGCCAGCGGGTCGTCGACATGCTC
GCCGACCTGCAGCGCGACCAGTCGGCGCTGCGCGCGCACCAGCATCTCGGGCTGCAGGAG
GTGCAGGCCGTCGTCGGGCCCGGAGCGGTCTTCGACACGCTGGTCATCTACGAGAACTTC
CCGCGTACGGGCCTCGGCGGATCGGCCGACGACGGCCTGGTCATGCGGCCGGTGCAGAAG
GGGCGCGACTCCTCGCACTACCCGTTCACACTGGTCACCGGTCCGGGCGAGCGGATGCCG
GTGATCCTCTACTACGACCGGGGGCTGTTCGACCAGGCGGCCGCCACATCGGTGCTCGGC
GCGATCGTCCGCGTGCTGGAGCGGCTGGTCGCCGAGCCCGGTGTGCTCGTCGGCCGGCTG
ACGCTGCTGGGCGAGGCCGAGCGCGCCGTGGTGGTCGGCGACTGGAACGCGACCGCGGGC
CCGGTCCCCGGCGACTCCATGGTCGAGCTGTTCGACCGGCGGGTGGCCGCCGCGCCGGAC
ACGGTGGCGATCACCGACGCCGACGGCGCCGACCTCACCTATGCCGAGGTCGATCAGGCG
TCGAACCGGTTGGCGGCCCACCTCGCCGATCGGGGCGTCCGCCGCGGCGACCGCGTCGGG
GTGGCCGTGGAGCGGTCCGCGGACCTGCCGATCGTGTTCCTGGCGATCTGGAAGGCCGGC
GCCGCGTACGTCCCGGTGGACGTCGGGTACCCGGCCGAGCGGATCGCGCTGATCCTCGCC
GGCTCCGCGGTCTCGACGGTGATCTGTACGCAGGCCACCGAGGCGGCGGTGCCGCAGAAC
GTGCCGCAGCGCGCCACGATCGTCCTCGATGCGCCGCCGACGCGGGCGGCCGTGGACGCC
TGTGCCGGCACCGCGCCGACGTTCCGGCCGAGCGCCGCCGACCTGGCGTACGTCATGTAC
ACCTCGGGCTCCACCGGCGTCCCGAAGGGCGTGGCCGTCCCGCACGGCGCGGTCGCCGGC
CTGGCCGGCGACGCGGGCTGGCGGATCGGCCCCGGCGACGGCGTGCTGATGCACGCGACG
CACGTGTTCGACCCCTCGCTCTACGAGATGTGGGTGCCGCTGGCCACGGGTGGCCGGGTC
CTGGTGGCCGCTCCCGGAGTGGTCGACGCGGGCGGGATCCGGCAGGCCGTGGCACGCGGC
GCGACCGCCGTCCACCTCACCGCCGGCACCTTCCGCGCGCTGGCGGAGGCGTCCCCGGAC
TGTTTCGCCGGCCTGCGCGAGGTCGGCACCGGCGGCGACGTGGTGCCCGCGCACACCGTG
GCCCACCTGCGGCGGGCCCAGCCACAGCTGCGGGTGCGCAACACGTACGGGCCGACCGAG
ACCACCCTGTGCGCCACCTGGAAGCCGATCGAGCCGGGCGCGCAGCTCGGGCCGGAGCTG
CCGATCGGCCGTCCGATGACCAACCGCAGGATCTACATCCTGGACGCCTTCCTGCGCCCG
GTCGCCCCCGGGGTGGCCGGCGAGCTGTACATCGCCGGCACCGGACTGGCCCGCGGATAT
CTCTCCCGGCCGGACCTGACCGCCGAGCGGTTCGTGGCGTGTCCGTTCCTCGCCGGCGAG
CGCATGTACCGGACCGGGGACCTGGCGCGCTGGAACCGCGACGGCGAGGTGGTCTTCCTC
GGGCGCGCCGACGACCAGGTGAAGATCCGCGGCTACCGGGTGGAGCTGGCCGAGGTGGAG
GCGGTGCTGGCGGCCCAGCCGGGAGTCCGCGAGGCGGTCGTCGTGGCCCGCGAGGACCGG
CCGGGCGAACGGCGTCTGGTCGGCTACGTCGTCTCCGACGCCGGTGAGCTGGACACCGAA
CAGATCCGCCAGCAGATGGCCCGGGTGCTGCCCGGCTACATGGTTCCGGTGGCGGTCATC
GGGCTGGTCAGCCTGCCGATCACCGCCAACAGCAAGGTGGACCGCCGGGCCCTGCCCGCC
CCGGACCTCGCCGGCCACGCGTCGGCGAAGGCGCCGGAGAGCGCGACCGAGAAGGTGCTG
TGCGCGCTCTACGCCGAGATCCTCGGCGTCGAGCGGGTGGGCGTCGACGACGCCTTCCAC
GACCTGGGCGGCAGTTCGGCGCTGGCCATGCGGCTGATCGCGCGGATCCGCGAGGAGCTG
GGCGTGGACCTGCCGATCCGCCAGCTGTTCTCCTCGCCCACACCCGCGGGGGTGGCCCGG
GCGCTGGCCGCGAAGTCGCGCCCCGCGCTGGAGGCGGTCACCCGGCCGGAGCGGGTGCCG
CTCACCGCCCGGCAGCTGCGCGCCTGGCTGCTGGCCCGCCCGTCGGAGGAGACCCGGGGC
CGGCACCTCTCGGTCGCGCTGCGCCTGCGCGGCCGGCTGGACGTGGCCGCCCTGGAGGCG
GCGCTGCGCGACGTCGCGGCCCGGCACGAGATCCTGCGGACCACCTTCCCGGGCGACGCG
CAGACCGTCCACCAGCACATCCACGACGCCGCGCCGGTCCGGCTGACGCCGGTCCCGGCC
ACCGAGGAGGACCTCCCCGCACGGCTGGCCGAGCGGGGCGAGCAGCTGTTCGACCTCACC
CGGGACATGCCGTGGCGGTGCGAGCTCTTCGCGCTGTCGGAGAAGGAGCACGTGCTGTCG
GTGACGGTGCACCGCATCGCCGCCGACGACGACTCGATGGACGTGTTCTTCCGGGACCTC
GCGGCCGCGTACGGCGCGCGGCGGGCCGGCCGGGCCCCGGAGCGCGCGCCGCTGGCCCTG
CAGTTCGCCGACTACGCGATCTGGGAGCAGCGGCTGCTCGACGGCGAGCGCGAGCAGGAC
AGCCTGATCAACGACCAGATCACCTTCTGGCGCAACCACCTGGCCGGCATCGACCAGGAG
ACCGTGCTGCCGTTCGACCGAGCGCGGCCGGCCATCCCGTCGCGCCGGGCCGGCACGGTC
GCGCTGCGGCTGGACGCCGGTCCGCACGCCCGGCTGGCCGAGGCGGTGGAATCGGCCGGC
GCGGACATGCCGCAGCTCGTGCAGGCCGCGCTGGCCATGCTGCTGACCCGGTACGGCGCG
GGCACCGACCTGGTGATCGGCACGACGCTGCCGCGCGACGAGGACCTGATCGACCTGGAG
CCGATGATCGGGCCGTTCGCCCGGCCGTTCCCGGTGCGTACCGACCTGTCGGCGGATCCG
ACCTTCCTGGAGGTCGTCGCCCGGGTGCAGGAGGCGGTCCGCGAGGCGCGCCAGCACCTG
GACGTGCCCTTCGAGAAGATTCCCGAGCTGCTGGCCCTGCCCGGCTCGCTGTCGCGCCAC
CCGGTGTACCAGGTCGGGCTGCAGGTGCGCGAGGAGGACAACGGCGCGTGGGACGCCGCG
GAACTGCCCGCCCTGCGCACCAGCGTCGAACCCACCGGGGTCGAGGCCATCGAGCTGGAC
CTGGCGTTCGCGCTCACCGAGCGCCGCAACGACGATGACGACGAGGACGGCATCGAGGGC
GCCCTGCACTATGCCGCCGACCTGTTCGACCACGACACGGCCGCGTCGCTGGCCCGGCGG
CTGGTCCGCGTCCTGGAACAGGTGGCCGAGGATCCCGGGCGGCGGATCAGCGACCTGGAC
ATCCTCCTCGACGACGCCGAGCGCGGCGCTCCGGCGATCGCGCCGGCGCGCTGGGCCGGG
GCGGTGCCGGCGGCGGTCGCCGACCTGGTCGCGGCCGGCCCGCTCGGTGCGCTGGTGCTC
GACGAGGCACAGCGCCCGGTGGCGCCCGGGACCGTCGGCGACCTGTGGGTGACCGGTCCC
GCGGTGGACGCGGACACCGGCGGCCGGCCGAGCGTGCCGTGTCCGTTCGGGACCCCGGGG
CACCGGATGCTGCACACCGGTCTGCTCGCCCGTAAGTCGCCGGCCAGGACGCTGGTCGTC
GTGGGGGAGCGGCGCGGGGCGAGCGCCTCGGCGAAGACCGGTGACTACGAGGTGCTGCTG
CCGCTGCGCGCCGCGGGTGACCGGGCGCCGCTGTTCTGCATCCACGCCAGCGGCGGCCTG
AGCTGGAACTACGGTCCGCTGCTGCGGCACCTGCCGCCGAACCAGCCGGTCTACGGGGTG
CAGGCGCGTGGCCTGGCCCGTACCGAGCCGCTGCCCGGCAGCGTGGAGGAGATGGCCGCC
GATTACGTCGAGCAGATCCGCACCGTGCAGCCGACCGGGCCGTACCACCTGCTCGGCTGG
TCTCTGGGCGGGCGGATCGCGCAGGCGATGGCCGTGCTGCTGGAGGCGGACGGGGAGAAG
GTCGGCCTGCTCGCCCTGCTCGCGGCCTATCCCGTCTACATGGGAAGGAAGGCCACCGGC
ACGGTCACCGAGGAGGCCGCCCGCGACGAAGACGCCCTGGAACGGCGCAAGCAGCAGGAA
CTGGAGCTCGCCGGTCAGCTGGTCCAGGGGGCCGGCGCCCGGTCCCGCTTGGAGGCGGTC
ATGCGCAACCTCTGGGAGGTCGGGCCGGCCCACACCGCCTCGCCGTTCGCCAGTGATGTC
CTCCTCTTCGTCGCCTCCGTGGACCGTCCCGCGCATCTGCCGGTGTCCGAGGCGATAGCC
AGCTGGAAAGACTTCACCGGGGGGACGATCGAGCCCCACGAGATCGCGACCAACCACTAC
GAGATGGTGCAGCCCGCGGCGTTGGCCCAGATCGGGGCCATCGTCGCCGAGAAACTCCGG
TCCCGGCCGGATGCGTGA
[7] C15..310
[7] A493..894
[7] L-3,5-dihydroxyphenylglycine(Dpg)664..764
[7] PCP976..1043
[7] X1057..1356
[7] TE1630..1850
[7] C43..930
[7] A1477..2682
[7] L-3,5-dihydroxyphenylglycine(Dpg)1990..2292
[7] PCP2926..3129
[7] X3169..4068
[7] TE4888..5550

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[493-894] 3.19999999999996e-106 PF00501
PF00501   AMP-binding
IPR001031 Thioesterase (Domain)
[1630-1850] 7.29999999999998e-34 PF00975
PF00975   Thioesterase
IPR001242 Condensation domain (Domain)
[15-310] 2.30000000000004e-65 PF00668 [1057-1356] 1.6e-54 PF00668
PF00668   Condensation
IPR009081 Acyl carrier protein-like (Domain)
[979-1042] 2.80000000000001e-13 PF00550
PF00550   PP-binding
[967-1046] 2.1e-27 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[976-1043] PS50075
PS50075   ACP_DOMAIN
[969-1044] 1.50000306971104e-19 SSF47336
SSF47336   ACP_like
IPR010071 Amino acid adenylation domain (Domain)
[493-894] 1.9e-129 TIGR01733
TIGR01733   AA-adenyl-dom
IPR020845 AMP-binding, conserved site (Conserved_site)
[618-629] PS00455
PS00455   AMP_BINDING
SignalP No significant hit
TMHMM No significant hit
Teicp2_00200 Teicp2_00200   Teicp2_00220 Teicp2_00220
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