Teicp2_00430 : CDS information

Location

Organism	Actinoplanes teichomyceticus
Strain	ATCC 31121 (=NBRC 13999)
Entry name	Teicoplanin
Contig	AJ605139
Start / Stop / Direction	77,016 / 78,377 / + [in whole cluster] 77,016 / 78,377 / + [in contig]
Location	77016..78377 [in whole cluster] 77016..78377 [in contig]
Type	CDS
Length	1,362 bp (453 aa)

Annotation

Category	4.3 transport
Product	putative transporter
Product (GenBank)	membrane ion antiporter
Gene
Gene (GenBank)	tcp34
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q6ZZH2 PmId [14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004) comment ATCC 31121株teicoplanin生合成gene clusterの同定論文。 tcp ORF34: Integral membrane transporter ORF34はABC-type transporterをコードすると予測されるので、排出機能に特定されそうである。配列解析のみ。
Related Reference	ACC Q6ZZH2 NITE Teicp_00360 PmId [15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004) comment ATCC 31121株でのtcp ORF34とAA配列完全一致。 UniProt同一entry. --- DSM 43866株teicoplanin生合成gene clusterの同定論文。 tei orf21: Na(+)-H(+) antiporter Orf21は多くのion transportersに似ている。類似遺伝子が他のglycopeptide gene clustersにも同定されているが、その機能は確かでない。配列解析のみ。
Related Reference	ACC Q939X8 NITE Balhi_00270 PmId [21690280] Self-resistance and cell wall composition in the glycopeptide producer Amycolatopsis balhimycina. (Antimicrob Agents Chemother. , 2011) comment BLAST id57% Amycolatopsis balhimycina Putative antiporter orf7に相当するentry. balhimycin生合成gene cluster内でグリコペプチド耐性に関与するかもしれない遺伝子として、orf7, vnlR, vnlS, vanYbを同定。 orf7はNa+/H+ antiporter geneに50%以上の相同性あり。しかしorf7のin-frame deletion mutantは、balhimycinの産生と耐性に影響しなかった。
Family/Domain	FD IPR006153 comment [IPR006153] Cation/H+ exchanger (Domain) Molecular Function GO:0015299 solute:proton antiporter activity

Sequence

>putative transporter [membrane ion antiporter]
MSFEAPPSSLGGHALLAFLIAVATLLLVAHLLARLAERVRMPAVVGELATGVILGPSLLG
HLLPGLTDWVFPPGTDQMHLLDAVGQIGILLLVGLTGTHLDVAMVRRRKATAARISLGGL
LLPLALGVGVGYQLAGWLSGAVVGDRLVFAGFMGVAMCVTAIPVIAKTLSDMRLLHRDVG
QMTLAAGTIDDAVGWFLLSVVSMAATAGLTTGHVVQAVAYLLGFVVLAVLLGRPLVRRVM
RLADRGEGSGSSIVTAVVVVLTGAAITQALGMEPVFGAFVAGILVGLPAAANQAKLAALR
TVVLSVLAPLFLATAGFRMDLTALADPQVAIAAVLVLAIAIVGKFAGAYAGARLSRLSRW
EGLAIGAGMNSRGVVEVVVALTGLRLGVLNTATYTIVVLVAIVTSVMAPPLLRYACARIA
QNEDERLRKLHHDTWNGVPAGAGQSPSPAGEQA

>putative transporter [membrane ion antiporter]
ATGTCCTTCGAAGCGCCGCCGTCGTCACTGGGCGGCCATGCCCTGCTCGCTTTCCTGATC
GCGGTGGCGACGCTGCTGCTGGTCGCCCACCTGCTGGCCCGCCTGGCGGAACGGGTGCGG
ATGCCGGCCGTCGTGGGTGAGCTGGCCACCGGCGTCATCCTGGGCCCGTCCCTGCTGGGC
CACCTGCTGCCCGGACTGACCGACTGGGTCTTCCCGCCCGGCACCGACCAGATGCACCTG
CTCGACGCGGTAGGTCAGATCGGCATCCTGCTCCTGGTCGGACTCACCGGCACCCACCTG
GACGTCGCCATGGTGCGGCGGCGCAAGGCCACCGCCGCGCGGATCAGCCTGGGCGGCCTG
CTGCTGCCGCTGGCTCTCGGGGTCGGCGTCGGCTACCAGCTGGCCGGCTGGCTCAGCGGC
GCCGTGGTCGGTGACCGGCTGGTCTTCGCCGGATTCATGGGCGTCGCGATGTGCGTGACA
GCCATCCCGGTGATCGCCAAAACCCTCTCCGACATGCGGCTGCTGCACCGCGACGTGGGC
CAGATGACGCTGGCGGCGGGCACCATCGACGACGCGGTCGGCTGGTTCCTGCTGTCCGTG
GTGTCGATGGCCGCGACCGCCGGGCTCACCACCGGACACGTCGTCCAGGCGGTCGCCTAC
CTGCTCGGCTTCGTCGTCCTCGCGGTTCTGCTCGGCCGGCCGCTGGTCCGCCGCGTCATG
CGGCTGGCCGACCGCGGCGAGGGATCCGGCTCCAGCATCGTCACCGCGGTCGTCGTCGTC
CTGACCGGCGCGGCGATCACCCAGGCGCTGGGCATGGAGCCGGTCTTCGGCGCCTTCGTG
GCGGGCATCCTGGTCGGGCTGCCCGCGGCGGCGAACCAGGCGAAGCTGGCGGCGCTGCGC
ACGGTCGTGCTCAGCGTGCTGGCGCCGCTCTTCCTCGCCACGGCCGGCTTCCGGATGGAC
CTGACCGCGCTGGCCGACCCGCAGGTCGCGATCGCCGCGGTGCTCGTGCTGGCCATCGCC
ATCGTCGGCAAGTTCGCCGGGGCGTACGCCGGGGCCCGCCTCAGCCGGCTGTCCCGCTGG
GAGGGCCTGGCGATCGGCGCCGGGATGAACTCGCGCGGCGTGGTCGAGGTGGTGGTGGCG
CTCACCGGCCTGCGACTGGGCGTGCTGAACACCGCCACCTACACGATCGTCGTCCTGGTC
GCCATCGTCACCTCGGTGATGGCCCCGCCGCTGCTGCGCTACGCCTGTGCCCGCATCGCC
CAGAACGAGGACGAGCGGCTGCGCAAGCTGCACCACGACACCTGGAACGGCGTCCCGGCC
GGCGCCGGCCAGTCCCCCTCACCCGCAGGAGAGCAGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006153 Cation/H+ exchanger (Domain) PF00999 Na_H_Exchanger
SignalP	No significant hit
TMHMM	Transmembrane 12

Teicp2_00420 Teicp2_00440