A4793_00050 : CDS information

Location

Organism	Streptomyces toyocaensis
Strain	NRRL 15009
Entry name	A47934
Contig	U82965
Start / Stop / Direction	7,541 / 6,312 / - [in whole cluster] 7,541 / 6,312 / - [in contig]
Location	complement(6312..7541) [in whole cluster] complement(6312..7541) [in contig]
Type	CDS
Length	1,230 bp (409 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450
Product (GenBank)	StaJ
Gene	staJ oxyC ORF30
Gene (GenBank)
EC number
Keyword	4th cross-linking AB ring
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)	P450-related oxidase
Reference	ACC Q8KLM1 PmId [12060705] Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009. (Proc Natl Acad Sci U S A. , 2002) [17884639] The biosynthesis of teicoplanin-type glycopeptide antibiotics: assignment of p450 mono-oxygenases to side chain cyclizations of glycopeptide a47934. (Chem Biol. , 2007) comment [PMID: 12060705](2002) Streptomyces toyocaensis NRRL15009由来A47934生合成gene clusterの同定論文。 34のORFsからなるclusterは、A47934の生合成とその調節に必要だと予測される遺伝子をすべて含む。グリコペプチド耐性を担う酵素をコードするORFsも含む。 StaJ: P450-related oxidase balhimycin生合成cluster gene(OxyC)と高い相同性があることから、AA5-7間の架橋形成が割り当てられている。 --- [PMID: 17884639](2007) 架橋形成に関するP450 monooxygenasesの調査。 mutants(ΔstaF, ΔstaG, ΔstaH, ΔstaJ）に蓄積した中間体の解析により、各架橋形成は特異的なoxygenaseへ明白に割り当てられ、環化の順番も決定された。 1) C-O-D by StaH 2) F-O-G by StaG 3) D-O-E by StaF 4) AB by StaJ

Sequence

>cytochrome P450 [StaJ]
MDEADEVAPVVRPTANYMRRTLCDPHEDMFALRAHGPLIRIEGNASDQMSTDYVWQAMGY
DVVRKILGDHENFTTRLRLTDAQPLSGEGVSVPPELAGQISIYDPPEHTRLRRMLTPEFT
VRRIRRLEPAIEGIIEEHLDALEGAGPPADLQVLFADPVGGETLCELLGVPRDDRNEFIR
RVRQNVDLSRGYKARAADSAAFNRYLMTLITRQRKDPDEGFLGMLVREHGDRITDEELKG
VCTALILGGVESVAGMIGFGVLALLEHPDQRRLLFGSREEADRLVNELLRFLSAVQQPTP
RMAVRDVVVEGQLIKAGEYVLCSILMANRDEGLTSDSHLLDANREPLPHVAFGHGIHHCI
GAAVARAVLRITYQSLWRRFPRLSLAVPAGEVKFRNAFIDSPDRLPVTW

>cytochrome P450 [StaJ]
GTGGATGAGGCGGACGAGGTCGCCCCGGTCGTCCGGCCGACGGCGAACTACATGAGAAGA
ACGCTCTGCGATCCGCACGAGGACATGTTCGCCCTCAGGGCGCATGGCCCGCTGATCCGG
ATCGAGGGCAACGCGTCCGATCAGATGAGCACGGACTACGTCTGGCAGGCCATGGGCTAC
GACGTGGTGCGCAAGATACTCGGCGATCATGAGAACTTCACGACGCGGCTCCGGCTGACC
GATGCGCAACCCCTCAGTGGCGAAGGGGTGTCGGTCCCGCCGGAGCTGGCCGGACAGATC
TCGATCTACGATCCGCCCGAACACACGCGGCTGCGGCGGATGTTGACGCCCGAGTTCACG
GTCCGGCGGATCCGCAGGCTGGAGCCGGCGATCGAGGGCATCATCGAAGAGCACCTCGAT
GCGTTGGAGGGCGCGGGACCGCCTGCGGATCTCCAGGTGCTGTTCGCCGACCCGGTCGGC
GGCGAGACCCTGTGCGAGCTGCTCGGGGTCCCGCGGGACGATCGGAACGAGTTCATCCGC
CGCGTCAGGCAGAACGTCGACCTCAGCCGCGGCTACAAGGCCAGGGCGGCGGACAGCGCG
GCCTTCAACCGATACCTGATGACGCTCATCACCCGGCAGCGGAAGGATCCCGACGAGGGG
TTCCTCGGCATGCTCGTACGTGAGCACGGCGACCGGATCACCGACGAGGAGTTGAAGGGC
GTCTGCACGGCGCTGATCCTCGGCGGCGTCGAGAGCGTCGCCGGGATGATCGGCTTCGGG
GTCCTCGCGCTGCTCGAGCACCCCGACCAGAGGCGGCTGCTCTTCGGTTCCCGCGAGGAG
GCCGACCGCCTGGTGAACGAACTGCTGCGTTTCCTTTCGGCCGTGCAGCAGCCGACTCCG
CGAATGGCCGTCAGGGACGTCGTCGTGGAGGGGCAGTTGATCAAGGCGGGGGAGTACGTC
CTCTGCTCGATTCTCATGGCCAACCGCGACGAGGGGCTGACGTCGGATTCCCATCTCCTC
GACGCGAACCGTGAACCCCTCCCGCATGTCGCCTTCGGGCACGGCATCCATCACTGCATA
GGCGCCGCAGTGGCAAGGGCGGTGCTCCGCATCACCTATCAGTCCCTGTGGCGACGGTTC
CCGCGACTGAGCCTGGCCGTGCCCGCGGGGGAAGTGAAGTTCCGTAACGCGTTCATAGAC
AGCCCGGATCGCTTGCCGGTCACCTGGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 SSF48264 Cytochrome P450 G3DSA:1.10.630.10 no description IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

A4793_00040 A4793_00060