A4793_00320 A4793_00320   A4793_00340 A4793_00340

A4793_00330 : CDS information

close this sectionLocation

Organism
StrainNRRL 15009
Entry nameA47934
Contig
Start / Stop / Direction66,891 / 67,517 / + [in whole cluster]
66,891 / 67,517 / + [in contig]
Location66891..67517 [in whole cluster]
66891..67517 [in contig]
TypeCDS
Length627 bp (208 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
ProductD-alanyl-D-alanine dipeptidase
Product (GenBank)D-ala-D-ala dipeptidase
GenevanXst
ORF1
Gene (GenBank)vanXst
EC number3.4.13.22
Keyword
Note
Note (GenBank)
  • VanXst
Reference
ACC
PmId
[9751644] Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function. (Chem Biol. , 1998)
[12060705] Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009. (Proc Natl Acad Sci U S A. , 2002)
comment
[PMID: 12060705](2002)
Streptomyces toyocaensis NRRL15009由来A47934生合成gene clusterの同定論文。

34のORFsからなるclusterは、A47934の生合成とその調節に必要だと予測される遺伝子をすべて含む。グリコペプチド耐性を担う酵素をコードするORFsも含む。

VanXst: D-Ala-D-Ala dipeptidase

clusterの5'末端にあるvanHst, vanAst, vanXstは抗生物質耐性遺伝子で、これらはペプチドグリカン層のD-Ala-D-AlaをD-Ala-D-lactateに変換してA47934にホスト耐性を与えるのに必要十分である。実験については以下の論文を引用している。

---
[PMID: 9751644](1998)
・Streptomyces toyocaensis (StoVanX)
・Escherichia coli (EcoVanX)
・Synechocystis sp. strain PCC6803 (SynVanX)
におけるvanX homologsを明らかにし、D,D-dipeptidase触媒活性を検証。

StoVanXは高効率なD-Ala-D-Ala dipeptidaseであり、D-Ala-D-lactate depsipeptide活性を欠く。
Related Reference
ACC
Q6ZZK1
NITE
Teicp2_00130
PmId
[17220405] Resistance to glycopeptide antibiotics in the teicoplanin producer is mediated by van gene homologue expression directing the synthesis of a modified cell wall peptidoglycan. (Antimicrob Agents Chemother. , 2007)
comment
BLAST id79%
Actinoplanes teichomyceticus_tcp4
Dipeptidase(VanX protein)

teicoplaninの産生株A. teichomyceticus ATCC 31121における自己耐性の包括的研究。

vanHat, vanAat, vanXat genesはユニークなpolycistronic mRNAで同時転写されることが示された。
vancomycinまたはteicoplanineを添加してもvanXat転写物量にはほとんど影響がなかったことから、恒常的な転写が示唆された。(data not shown)

close this sectionSequence

selected fasta
>D-alanyl-D-alanine dipeptidase [D-ala-D-ala dipeptidase]
MKPTESMTDGFVFADDLVPGLRWDAKYATWDNFTGKPVDGYLVNRIVGTKALCAALERAR
DEAASLGFGLLLWDGYRPQRAVDCFLRWSQQPEDGRTKSRHYPNIARAEMFEKGYVAARS
GHSRGSTVDLTLYDLATNELVPMGGDHDLMDPVSHHGAQGLAPVEARNRQHLRSVMESCG
FKAYEYEWWHYTLADEPYPDTYFDFPIM
selected fasta
>D-alanyl-D-alanine dipeptidase [D-ala-D-ala dipeptidase]
ATGAAGCCGACGGAGTCCATGACGGACGGCTTCGTCTTCGCGGACGACTTGGTGCCCGGA
CTGCGCTGGGACGCCAAGTACGCCACGTGGGACAACTTCACCGGCAAACCTGTGGACGGA
TATCTGGTCAACCGAATCGTCGGTACGAAGGCTCTGTGCGCGGCTCTGGAGCGGGCGCGG
GACGAGGCCGCGTCACTGGGGTTCGGCCTGCTGCTCTGGGACGGCTATCGACCGCAGCGC
GCCGTCGACTGCTTTCTGCGCTGGTCGCAGCAGCCGGAGGACGGCCGGACGAAGTCGCGG
CACTATCCCAACATCGCCAGGGCCGAGATGTTCGAGAAGGGATATGTGGCCGCCAGGTCG
GGCCACAGCAGGGGCAGCACCGTCGACCTCACACTCTACGACCTGGCCACGAACGAGCTC
GTTCCCATGGGCGGCGACCACGACCTGATGGATCCCGTCTCGCACCACGGGGCACAAGGG
CTCGCCCCCGTCGAGGCGCGGAACCGTCAGCACCTGCGGTCCGTGATGGAGTCCTGCGGT
TTCAAGGCGTACGAGTACGAGTGGTGGCACTACACGCTCGCCGACGAGCCCTATCCGGAC
ACGTACTTCGATTTCCCCATCATGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000755 Peptidase M15D, VanX D-ala-D-ala dipeptidase (Family)
[8-206] 1.10000000000001e-68 PF01427
PF01427   Peptidase_M15
[1-208] 4.10001399699315e-59 PIRSF026671
PIRSF026671   AA_dipeptidase
IPR009045 Hedgehog signalling/DD-peptidase zinc-binding domain (Domain)
[8-207] 6.10000000000004e-65 G3DSA:3.30.1380.10
G3DSA:3.30.1380.10   Hedgehog/DD-pept_Zn-bd
[7-208] 4.69998262513189e-72 SSF55166
SSF55166   Hedgehog_sig_N
SignalP No significant hit
TMHMM No significant hit
A4793_00320 A4793_00320   A4793_00340 A4793_00340
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