Amph_00050 Amph_00050   Amph_00070 Amph_00070

Amph_00060 : CDS information

close this sectionLocation

Organism
StrainATCC 14899 (=NBRC 12895)
Entry nameAmphotericin B
Contig
Start / Stop / Direction50,571 / 56,675 / + [in whole cluster]
50,571 / 56,675 / + [in contig]
Location50571..56675 [in whole cluster]
50571..56675 [in contig]
TypeCDS
Length6,105 bp (2,034 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)AmphK
Gene
Gene (GenBank)amphK
EC number
Keyword
Note
Note (GenBank)
  • polyketide synthase multienzyme polypeptide housing extension modules 18 and thioesterase
Reference
ACC
PmId
[11451671] Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes. (Chem Biol. , 2001)
comment
Amphotericin生合成gene clusterの報告。
amphK: PKS protein, module 18-TE

Module 18のDH domainは明らかにnon-functional.

close this sectionPKS/NRPS Module

18 malonyl-CoA
KS34..407
AT553..864
dh915..1075
KR1386..1566
ACP1665..1735
TE1822..2026

close this sectionSequence

selected fasta
>polyketide synthase [AmphK]
MPDESKLVDYLKWVTADLHQTRRRLQEAESGRHEPVAIVGMACRFPGGVRSPEDLWEMLA
DGRDAISGFPADRGWDLETLAGDGAGGSSTQEGGFLHDVADFDPGFFDISPREALAMDPQ
QRLLLETAWEAVERAGIAPGSLRGSRTGVFVGTNSQDYAHLVLASDDDMGGYAGNGLAAS
VMSGRLSFALGFEGPAVTLDTACSSALVALHLAAQSVRSGEADLALAGGVTVMTTSSSFV
GFSLQGGLATDGRCKAFADSADGTGWSEGVGMILVERLSEARRKGHPVLAVLRGSAVNQD
GASNGLSAPNGPAQQRVIRDALASAGLSPADVDAVEAHGTGTTLGDPIEAQALLATYGQD
RDASRPLRLGTVKSNIGHTQAAAGAAGVIKMVLALRHGLLPRTLHIDAPSTHVDWDAGHV
SLLTEATPWPEGEQTRRAGVSSFGISGTNAHVILEEAPAAEEDTDAEQRPEPVVPGAVPW
PVSARTADALDAQLEKVRPLAASGADPVAVGHALAVTRTPFEHRALLVAADGNLTEAARG
TVPSGDRPGLAVLFSGQGAQRLGMGRELHARFPVFAAALDETLALLDERLGHSLRDVIWG
EDPVALDDTGHTQPALFAVEVALYRLFASWGLRPDHLAGHSVGEIAAVHVAGALSLEDAC
TLVAARAGLMRDLPAGGAMVALRATEAEVRPLLDERVSLASVNGPDSVVVSGAEDAVLAV
AEHFRKQDRRTTRLSVSHAFHSPLVDPMLDAFRDVVAKLTFGEPSVPVVSTLTGDVVAAE
ELATPHYWVCHARQAVRFADAVRTLVDEGARTFLEVGPGGVLSALVGENTQEAGVSAVPA
LRKDQPEEASVLAALGTLWTRGTAADWDAVFEGTLPGRPESVDLPTYAFQRGRYWPTVRA
RSGDPAGLGLGAAGHPLLSATVTLAEADESVLTGRLSPLTHPWLTEHRVDGRITVPGTAL
VEIAVRAGDENGTPRLDRLDLLAPLTLGDRDAVLLQVRVGPEDASGRRTLSVHARPATAD
DAPWTTYARGVLAPDEGAEDTAADLVEWPPADARQVPLTELESEGRALGPLFSGLTGVWR
HEGEVFAEAELPAGPDSGFGLHPALLSTALRAAAVLDGTTAGEPAAFEGLTLHATGATAL
RVRLSTTGPDTVDLTAVDPAGNLVLTAETVRLGTPDGTADTPAATGRGDLFGLKWVPVKA
SERATGTRWAVVGSDELDLGYAMHRADETVTAYAESLGGAIGDSGVAPDVFLIPLAGEKD
AGAEGVHALTTRVLGYLQEWLSEPRLSGTRLVFVTRRAVALDDEDVLDPAGAAVWGLVRS
AQTENPGSLLLVDLDDTFLSAGVLPDVLTLDEQQLAVRDYQVRAARLARLPRPADDAPAA
DWNPDGTVLITGGTGGLGAALARHLVTSRGARHLLLASRRGPDAPGTSELVAELTGLGAQ
VTVSACDVGDRDAVDTLVASVPAEHPLTAVVHTAGVLDDALTGSLTPEQLAGVLRPKADA
ALHLHEATLGQDLAAFVLYSSISGVIGGPGQANYAAANASLDALAHRRRAAGLPGLSLAW
GPWGRGSGMTSQVSDTDLGRMDRGGTPPMSLEDGLALFDAALARTEPMVVPTRINVTGLQ
VQQTLPALWRDLVPRPRRTAAADRSPKTVLDGLRTLDTAGREKLLTELVVGFTAGLLGHA
DPAAVDPERGFLELGFDSLVSVSLRNQLGELLGLRLPTSVVFDSKTPVKLARHLNEELGD
LSASGPASGTAVAGTTVHPDDTLVGLFHNAVRGGKLVEAMRMLKAVANTRPTFETPADLE
ELSEAVTLATGPGSPRLIFVSAPGATGGVHQYARIAAHFRGKRHVSAIPLMGFAPGELLP
ATSEAAARIVAESVLMASDGEPFVMVGHSTGGSLAYLAAGVLEDTWGVKPEAVVLLDTAS
IRYNPSEGNNLDQTTRFYLADIDSPSVTLNSARMSAMAHWFMAMTDIDAPATTAPTLLLR
ATQANNGFMLDTSAVPADVVRDIEADHLSLAMEHSDLTAEAIENWLAELPAGEA
selected fasta
>polyketide synthase [AmphK]
ATGCCGGACGAAAGTAAGCTCGTCGATTACCTGAAGTGGGTCACGGCGGATCTCCACCAG
ACCCGCAGGCGCCTCCAGGAAGCCGAGTCGGGCCGCCACGAGCCCGTGGCGATCGTCGGC
ATGGCGTGCCGCTTCCCCGGCGGTGTGCGTTCCCCGGAGGACCTCTGGGAGATGCTCGCC
GACGGCCGCGACGCCATCTCCGGGTTCCCCGCCGACCGCGGCTGGGACCTGGAGACGCTG
GCCGGCGACGGAGCGGGCGGCAGCAGCACGCAGGAGGGAGGTTTCCTGCACGACGTGGCC
GACTTCGACCCGGGGTTCTTCGACATCTCGCCCCGCGAGGCGCTGGCCATGGACCCGCAG
CAGCGGCTGCTCCTGGAGACCGCCTGGGAGGCCGTGGAGCGTGCCGGGATCGCCCCCGGC
AGCCTCCGCGGCAGCCGCACCGGCGTGTTCGTCGGCACCAACTCGCAGGACTACGCCCAC
CTCGTCCTCGCCTCCGACGACGACATGGGCGGCTACGCGGGCAACGGCCTGGCCGCCAGC
GTGATGTCCGGCCGGCTGTCCTTTGCGCTCGGCTTCGAAGGACCCGCCGTCACCCTCGAC
ACCGCGTGCTCCTCGGCCCTGGTCGCCCTGCACCTGGCCGCCCAGTCGGTGCGCTCCGGC
GAGGCCGACCTCGCGCTGGCCGGCGGTGTCACCGTCATGACCACCTCGTCCAGCTTCGTG
GGCTTCAGCCTCCAGGGCGGCCTCGCCACGGACGGCCGCTGCAAGGCGTTCGCCGACTCC
GCCGACGGCACCGGCTGGTCCGAGGGCGTCGGCATGATCCTCGTCGAACGGCTCTCCGAG
GCCCGGCGCAAGGGCCACCCGGTGCTCGCCGTGCTGCGCGGCTCCGCCGTGAACCAGGAC
GGCGCCTCCAACGGCCTCAGCGCCCCCAACGGGCCCGCCCAGCAGCGCGTCATCCGCGAC
GCGCTGGCCTCCGCCGGGCTCTCGCCCGCCGACGTCGACGCCGTCGAGGCGCACGGCACC
GGCACCACCCTCGGCGACCCCATCGAGGCGCAGGCCCTGCTCGCCACCTACGGCCAGGAC
CGCGACGCGAGCCGTCCGCTGCGCCTCGGCACGGTGAAGTCCAACATCGGCCACACCCAG
GCCGCCGCCGGCGCCGCGGGCGTCATCAAGATGGTCCTGGCCCTGCGCCACGGGCTGCTG
CCCCGCACCCTGCACATCGACGCGCCGTCCACACACGTCGACTGGGACGCAGGACACGTC
AGCCTGCTCACCGAGGCCACCCCCTGGCCCGAGGGCGAGCAGACGCGCCGGGCCGGTGTG
TCGTCCTTCGGCATCAGCGGCACCAACGCCCATGTCATCCTCGAAGAGGCCCCCGCGGCC
GAGGAGGACACCGACGCCGAGCAGCGGCCCGAGCCGGTCGTCCCGGGCGCCGTGCCGTGG
CCCGTGTCGGCCCGTACCGCCGACGCCCTCGACGCCCAGCTGGAGAAGGTGCGGCCGCTC
GCCGCGTCCGGCGCCGACCCCGTCGCCGTCGGCCACGCCCTGGCCGTCACCCGCACCCCC
TTCGAACACCGGGCGCTGCTGGTCGCCGCGGACGGCAATCTCACCGAGGCCGCACGCGGC
ACCGTCCCCTCCGGCGACCGCCCCGGGCTGGCCGTGCTGTTCTCCGGGCAGGGCGCCCAG
CGCCTCGGCATGGGCCGTGAACTCCACGCCCGCTTCCCGGTGTTCGCCGCCGCCCTGGAC
GAGACCCTCGCCCTCCTCGACGAGCGCCTCGGCCACTCGCTGCGCGACGTCATCTGGGGC
GAGGACCCCGTGGCCCTCGACGACACCGGCCACACCCAGCCCGCGCTGTTCGCCGTGGAG
GTCGCCCTCTACCGCCTGTTCGCCTCCTGGGGTCTGCGCCCCGACCACCTCGCCGGACAC
TCCGTCGGGGAGATCGCCGCCGTGCATGTGGCCGGCGCACTGTCCCTGGAGGACGCCTGC
ACCCTGGTTGCCGCCCGCGCCGGCCTGATGCGGGACCTGCCGGCCGGCGGAGCCATGGTC
GCGCTGCGCGCCACCGAGGCCGAGGTGCGGCCGCTGCTCGACGAGCGGGTCTCGCTCGCC
TCGGTCAACGGCCCCGATTCCGTGGTGGTCTCCGGCGCCGAGGACGCCGTCCTCGCCGTC
GCCGAGCACTTCCGGAAGCAGGACCGGCGCACCACGCGGCTCTCCGTGAGCCACGCCTTC
CACTCCCCGCTCGTCGACCCGATGCTCGACGCCTTCCGGGACGTCGTCGCGAAGCTCACC
TTCGGCGAGCCGTCGGTACCCGTCGTCTCCACCCTCACCGGCGACGTCGTCGCCGCCGAG
GAACTGGCCACGCCGCACTACTGGGTGTGCCACGCCCGGCAGGCCGTCCGCTTCGCCGAC
GCCGTGCGCACCCTCGTCGACGAGGGCGCCCGGACCTTCCTGGAGGTCGGCCCGGGCGGC
GTGCTGTCCGCGCTCGTCGGTGAGAACACCCAGGAGGCCGGGGTCTCTGCCGTGCCCGCG
CTGCGCAAGGACCAGCCGGAGGAGGCCTCCGTGCTCGCCGCCCTCGGCACCCTGTGGACC
CGGGGCACCGCGGCCGACTGGGACGCCGTGTTCGAGGGCACCCTGCCCGGCCGCCCGGAG
TCCGTGGACCTGCCGACCTACGCCTTCCAGCGCGGCCGGTACTGGCCCACCGTGCGCGCC
CGCTCCGGCGACCCCGCCGGACTGGGCCTCGGCGCCGCCGGGCACCCGCTGCTCAGCGCC
ACCGTCACCCTCGCCGAGGCCGACGAGTCCGTCCTCACCGGACGGCTCTCGCCGCTGACC
CACCCCTGGCTCACCGAGCACCGCGTCGACGGCCGGATCACCGTCCCCGGCACGGCCCTC
GTGGAGATCGCGGTCCGTGCGGGCGACGAGAACGGCACCCCCCGCCTGGACCGGCTCGAC
CTGCTCGCCCCGCTGACCCTCGGCGACCGCGACGCGGTGCTGCTCCAGGTGCGCGTCGGC
CCCGAGGACGCCTCCGGCCGGCGGACGCTCTCCGTGCACGCCCGCCCGGCGACCGCGGAC
GACGCCCCCTGGACCACGTACGCCCGTGGTGTCCTCGCCCCCGACGAGGGCGCCGAGGAC
ACCGCCGCGGACCTTGTGGAGTGGCCGCCCGCCGACGCCCGTCAGGTCCCGCTCACGGAG
CTGGAGTCCGAAGGACGCGCCCTCGGCCCGCTCTTCAGCGGCCTCACCGGGGTGTGGCGG
CACGAGGGCGAGGTGTTCGCCGAGGCCGAGCTGCCCGCAGGACCCGACAGCGGCTTCGGA
CTGCACCCCGCGCTGCTGTCCACCGCCCTGCGCGCCGCCGCCGTCCTCGACGGCACCACG
GCCGGCGAGCCCGCCGCCTTCGAGGGGCTCACCCTGCACGCCACCGGCGCCACCGCGCTG
CGCGTACGGCTGAGCACCACCGGGCCCGACACCGTCGACCTCACCGCCGTCGACCCCGCG
GGCAACCTCGTCCTGACCGCCGAGACCGTGCGTCTCGGCACCCCGGACGGCACCGCCGAC
ACCCCGGCCGCCACCGGCCGGGGCGACCTGTTCGGGCTGAAGTGGGTGCCCGTCAAGGCC
TCGGAGCGCGCCACCGGCACCCGCTGGGCCGTCGTCGGCTCCGACGAACTCGACCTCGGC
TACGCCATGCACCGCGCCGACGAGACCGTCACCGCCTACGCGGAGTCCCTCGGCGGCGCC
ATCGGCGACAGCGGGGTCGCCCCCGATGTGTTCCTGATCCCGCTCGCAGGCGAGAAGGAC
GCCGGAGCGGAGGGCGTGCACGCCCTCACCACCCGGGTCCTCGGCTACCTCCAGGAGTGG
CTGTCCGAGCCCCGGCTCTCCGGCACCCGCCTGGTCTTCGTCACCCGCCGCGCCGTCGCC
CTCGACGACGAGGACGTCCTGGACCCGGCCGGCGCGGCCGTCTGGGGCCTGGTGCGCTCC
GCGCAGACCGAGAACCCCGGCAGCCTGCTGCTCGTCGACCTCGACGACACCTTCCTGTCC
GCCGGAGTACTGCCCGACGTGCTGACGCTCGACGAGCAGCAGCTCGCGGTCCGCGACTAC
CAGGTCCGCGCCGCCCGGCTCGCCCGGCTGCCGCGCCCGGCCGACGACGCCCCCGCCGCC
GACTGGAACCCCGACGGCACCGTCCTGATCACCGGCGGCACCGGCGGCCTCGGCGCCGCG
CTCGCCCGGCACCTGGTCACCAGCCGGGGCGCCCGTCACCTGCTGCTGGCGAGCCGCCGC
GGCCCGGACGCGCCCGGCACGTCCGAACTGGTCGCCGAGCTGACCGGCCTGGGAGCCCAG
GTGACCGTCTCCGCCTGCGACGTCGGCGACCGCGACGCGGTCGACACGCTCGTCGCGTCG
GTGCCCGCCGAGCACCCGCTGACCGCCGTCGTGCACACCGCGGGTGTCCTGGACGACGCC
CTGACCGGCTCGCTCACCCCGGAACAACTGGCTGGTGTGCTGCGCCCCAAGGCCGACGCG
GCCCTGCACCTGCACGAGGCGACCCTCGGCCAGGACCTCGCCGCGTTCGTGCTGTACTCC
TCGATCTCCGGCGTCATCGGCGGCCCCGGCCAGGCCAACTACGCCGCCGCCAACGCCTCC
CTGGACGCGCTCGCCCACCGGCGCAGGGCGGCCGGTCTGCCCGGTCTGTCCCTCGCCTGG
GGGCCCTGGGGCCGCGGCAGCGGCATGACCAGCCAGGTCAGCGACACCGACCTGGGGCGG
ATGGACCGCGGCGGCACCCCGCCGATGAGCCTCGAGGACGGCCTCGCCCTGTTCGACGCC
GCCCTGGCCCGCACCGAGCCGATGGTCGTGCCCACCCGGATCAATGTCACCGGACTCCAG
GTCCAGCAGACGCTGCCCGCGCTCTGGCGCGACCTGGTGCCGCGCCCCCGGCGCACCGCC
GCCGCGGACCGCTCGCCCAAGACCGTGCTCGACGGGCTGCGCACGCTCGACACCGCGGGC
CGGGAGAAGCTGCTCACCGAGCTGGTCGTCGGCTTCACCGCGGGCCTGCTCGGCCACGCC
GACCCCGCCGCCGTCGACCCCGAGCGCGGCTTCCTGGAGCTGGGCTTCGACTCGCTGGTC
TCGGTCAGCCTGCGCAACCAGCTCGGCGAACTCCTCGGGCTGCGCCTGCCGACGTCGGTG
GTCTTCGACAGCAAGACGCCGGTGAAGCTGGCCCGCCACCTCAACGAGGAACTGGGCGAC
CTGTCCGCCTCCGGCCCCGCGTCCGGCACCGCCGTCGCCGGCACCACGGTGCATCCCGAC
GACACCCTGGTCGGCCTGTTCCACAACGCGGTGCGCGGCGGCAAGCTCGTCGAGGCGATG
CGGATGCTGAAGGCGGTCGCCAACACCCGGCCCACCTTCGAGACGCCCGCGGACCTGGAG
GAGCTGTCCGAGGCCGTCACCCTGGCCACGGGGCCCGGCTCCCCGCGGCTGATCTTCGTC
AGCGCGCCCGGCGCCACCGGCGGTGTCCACCAGTACGCGCGCATCGCCGCCCACTTCCGC
GGCAAGCGCCATGTGTCGGCGATACCGCTGATGGGCTTCGCCCCCGGCGAGCTGCTGCCC
GCCACCAGTGAGGCGGCGGCCCGGATCGTCGCCGAGAGCGTCCTGATGGCGAGCGACGGC
GAACCGTTCGTGATGGTGGGCCACTCCACCGGCGGTTCCCTCGCCTACCTCGCGGCCGGG
GTGCTGGAGGACACCTGGGGCGTGAAGCCGGAGGCGGTCGTCCTGCTCGACACGGCGTCC
ATCCGGTACAACCCCTCGGAGGGCAACAACCTCGACCAGACGACCCGCTTCTACCTCGCC
GACATCGACTCGCCGTCCGTGACGCTCAACAGCGCCCGGATGTCCGCGATGGCGCACTGG
TTCATGGCGATGACGGACATCGACGCGCCCGCCACGACCGCCCCCACCCTGCTCCTGCGC
GCCACACAGGCCAACAACGGCTTCATGCTGGACACCTCCGCGGTGCCGGCGGACGTGGTG
CGTGACATCGAGGCCGACCATCTGTCCCTGGCCATGGAGCACTCGGATCTGACCGCCGAG
GCCATAGAGAACTGGCTCGCCGAACTTCCGGCCGGCGAGGCCTGA
[18] KS34..407
[18] AT553..864
[18] malonyl-CoA738..742
[18] dh915..1075
[18] KR1386..1566
[18] ACP1665..1735
[18] TE1822..2026
[18] KS100..1221
[18] AT1657..2592
[18] malonyl-CoA2212..2226
[18] dh2743..3225
[18] KR4156..4698
[18] ACP4993..5205
[18] TE5464..6078

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[1822-2026] 5.90000000000001e-16 PF00975
PF00975   Thioesterase
IPR001227 Acyl transferase domain (Domain)
[545-673] 9.2000000000001e-77 G3DSA:3.40.366.10 [738-854] 9.2000000000001e-77 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[1386-1553] 2.4e-63 PF00106
PF00106   adh_short
IPR009081 Acyl carrier protein-like (Domain)
[1674-1734] 7e-10 PF00550
PF00550   PP-binding
[1657-1752] 7.49999605851445e-18 SSF47336
SSF47336   ACP_like
[1665-1739] 4.6e-16 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1665-1735] PS50075
PS50075   ACP_DOMAIN
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[34-281] 1.10000000000001e-92 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[289-407] 1.5e-47 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[553-864] 1.79999999999997e-70 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[1-27] 4.9e-15 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[549-848] 2.1000026783403e-72 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[675-737] 2.09999806691534e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[34-291] 1e-89 G3DSA:3.40.47.10 [293-459] 1.9e-65 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[26-405] 5.39997012872478e-100 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1386-1588] 3.40000000000004e-38 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[194-210] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[553-845] 7.60000011115808e-119 SM00827
SM00827   PKS_AT
IPR020802 Polyketide synthase, thioesterase domain (Domain)
[1818-2026] 5.6999970878395e-89 SM00824
SM00824   PKS_TE
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1666-1738] 9.5000102816722e-26 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[915-1075] 2.1000026783403e-58 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[36-459] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1386-1566] 5.30001985225035e-63 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Amph_00050 Amph_00050   Amph_00070 Amph_00070
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