Amph_00080 Amph_00080   Amph_00100 Amph_00100

Amph_00090 : CDS information

close this sectionLocation

Organism
StrainATCC 14899 (=NBRC 12895)
Entry nameAmphotericin B
Contig
Start / Stop / Direction59,610 / 58,756 / - [in whole cluster]
59,610 / 58,756 / - [in contig]
Locationcomplement(58756..59610) [in whole cluster]
complement(58756..59610) [in contig]
TypeCDS
Length855 bp (284 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative aminodeoxychorismate lyase
putative 4-amino-4-deoxychorismate lyase
Product (GenBank)AmphORF2
GeneORF2
Gene (GenBank)amphORF2
EC number4.1.3.38
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11451671] Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes. (Chem Biol. , 2001)
comment
Amphotericin生合成gene clusterの報告。
ORF2: Hypothetical protein
Related Reference
ACC
Q6W5R4
NITE
FR008_00020
PmId
[19389784] Functional characterization of the first two actinomycete 4-amino-4-deoxychorismate lyase genes. (Microbiology. , 2009)
comment
50th(Q6W5R4) 38%, 5e-33
Streptomyces sp. FR-008_pabC
[FR008_00020]aminodeoxychorismate lyase

pabC-1(=pabC)の不活化でFR-008産生はwildの20%まで減少。
pabC-1はE.coli pabC mutantを相補できる。

同じ株からpabC-2も同定され（FR-008 cluster外のようだ）、
不活化でFR-008産生をwildの57%まで減少。E.coli pabC mutant相補可能。

pabC-1/pabC-2 double mutantはFR-008産生をwildの4%しか保持せず。
in vitroでの個々の活性を測定。どちらも機能的なaminodeoxychorismate(ADC) lyasesであった。

pabC-1/pabC-2どちらも一次代謝、二次代謝の両方に関与するADC lyasesである。

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この論文で述べられているADC lyaseに必須であるとされているconserved residues 3つはAmphORF2でも保存されている。

close this sectionSequence

selected fasta
>putative aminodeoxychorismate lyase [AmphORF2]
MAAGLGVGEGGVGIDRVEVNGVEADSEDLKVLMRVSYAHFTSMQVRGGAVRGLDLHLRRL
DESARGLFGRGLDAERVRGCVRHALEGGPEAVSVRVTVFSRRLDAVLRGEAVEPELAVAT
SAPAEAQSEPMRLRAVEYERDLPHVKHVGTFGLIHGRRQAVLSGYDDVLFTDRYGRISEA
SVWNIGFYDGERVIWPEAAVLPGITMQLLQRGLEAKGIPSERREVRLDDIVARASGSPVL
AAFLTNSISPALPVASIDGAALAVESAVTDLLVDCYESNPWETV
selected fasta
>putative aminodeoxychorismate lyase [AmphORF2]
ATGGCAGCCGGGCTGGGTGTGGGTGAGGGAGGGGTTGGCATCGACCGGGTGGAAGTCAAC
GGTGTCGAGGCGGACAGCGAGGACCTCAAGGTGCTCATGCGGGTCAGCTACGCGCATTTC
ACCTCGATGCAGGTGCGGGGCGGTGCGGTCCGGGGGCTGGATCTGCACCTGAGACGGTTG
GACGAGAGCGCGCGCGGGTTGTTCGGCAGGGGCCTGGACGCCGAGCGGGTGCGGGGATGT
GTGCGGCACGCCCTCGAAGGGGGCCCGGAGGCGGTATCGGTCCGGGTGACCGTCTTCTCC
CGGAGGCTGGATGCCGTGCTGCGCGGCGAGGCCGTGGAGCCGGAGCTGGCGGTGGCGACG
AGCGCCCCGGCTGAGGCGCAGAGCGAGCCGATGCGACTGCGGGCGGTGGAGTACGAACGG
GACCTGCCTCATGTGAAACACGTGGGGACGTTCGGGCTCATTCATGGCCGACGGCAGGCG
GTGCTCTCCGGGTACGACGACGTACTGTTCACCGATCGGTACGGCCGGATCAGCGAGGCG
TCGGTCTGGAACATCGGCTTCTACGACGGGGAGCGAGTGATCTGGCCGGAGGCCGCGGTG
CTGCCGGGCATCACGATGCAGCTGCTGCAGCGGGGGCTGGAGGCCAAGGGCATCCCCTCA
GAGCGGCGTGAGGTCCGGCTCGATGACATCGTGGCCCGTGCGTCCGGCTCCCCGGTCCTC
GCTGCCTTCCTGACGAACTCGATCTCGCCGGCCCTTCCGGTCGCGAGCATCGACGGGGCA
GCACTGGCCGTCGAATCGGCGGTCACGGACCTGCTCGTGGACTGCTACGAGAGCAATCCC
TGGGAAACCGTCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001544 Aminotransferase, class IV (Family)
[11-277] 1.3000049540733e-33 SSF56752
SSF56752   Aminotrans_IV
[53-271] 2.5e-23 PF01063
PF01063   Aminotran_4
SignalP
[1-2] 0.067 Signal
Eukaryota   
[1-38] 0.039 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Amph_00080 Amph_00080   Amph_00100 Amph_00100
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