Conca_00180 Conca_00180   Conca_00200 Conca_00200

Conca_00190 : CDS information

close this sectionLocation

Organism
StrainATCC 27449 (=NBRC 13477)
Entry nameConcanamycin A
Contig
Start / Stop / Direction89,325 / 87,823 / - [in whole cluster]
89,325 / 87,823 / - [in contig]
Locationcomplement(87823..89325) [in whole cluster]
complement(87823..89325) [in contig]
TypeCDS
Length1,503 bp (500 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose 2,3-dehydratase
Product (GenBank)2,3-dehydratase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF 10*
Reference
ACC
PmId
[16207901] Organization of the biosynthetic gene cluster for the macrolide concanamycin A in Streptomyces neyagawaensis ATCC 27449. (Microbiology. , 2005)
comment
ORF10* NDP-hexose 2,3-dehydratase

ORF5*-11*はconcanamycinAのsugar moietyである4'-O-carbamoyl-2'-deoxyrhamnoseの合成と付加に関与していると考えられている。
ORF10*はdTDP-sugar-2,3-dehydrataseにとても相同性のある遺伝子産物をコードする。
Fig. 3で提唱されている4'-O-carbamoyl-2'-deoxyrhamnose生合成経路において、NDP-4-keto-6-deoxy-D-glucoseを基質とした脱水反応に当てはめられている。
Related Reference
ACC
Q9ALN6
NITE
Spino_00070
PmId
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
23rd(Q9ALN6) 52%, 1e-123
Saccharopolyspora spinosa_spnO
[Spino_00070]dTDP-4-keto-6-deoxyglucose 2,3-dehydratase

dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO

dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。
dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。

--
SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

close this sectionSequence

selected fasta
>putative NDP-hexose 2,3-dehydratase [2,3-dehydratase]
MTASPVTPGGLLRTRDPSAAPRVVRSALSTQGTVTATPDFRSWFAERLAVARTTTVAGID
FADLVGWRFADDTGNLVHDSGKFFTIEGLSVAAAHGPVASWSQPIINQPETGVLGILVKE
FDGVLHCLMQAKMEPGNCNVLQLSPTVQATQSNYTAVHRGRPVPYVDMFLSAPPQSVLAD
VLQSEQGSWFYRKRNRNIVVETTADVEVLPGFCWLSLGQVHALLTEDHLVNMNTRTVLSC
LSFSGPNLVGELGCGASGFRAALVRSIETAWGALHTDGEILSWITDLRSRREVEATPIPL
CEVRGWRRLPDRITHDTGVFFDVRAVAVDGGGREIDSWTQPVIAPHGLGVVAFLVARFSG
VLHVLVGVQVEPGLLDFVELGPTVQCTPDSYEHLPAEARPPFLDDVLAAPADRIRFDTEL
SEEGGRFFHARTRHMIVEVDPVVAHGAGPARHRWMTMAQIVGLLRHSHYVNVQARSLVAC
LHSLVARLDDDSPVAPGGGA
selected fasta
>putative NDP-hexose 2,3-dehydratase [2,3-dehydratase]
ATGACCGCATCCCCGGTCACCCCCGGTGGTCTACTTCGGACCCGCGACCCGTCGGCGGCG
CCGCGTGTCGTCCGGTCGGCACTGTCCACCCAGGGCACCGTGACGGCCACACCGGACTTC
CGGTCCTGGTTCGCCGAACGGCTGGCCGTGGCCAGGACCACCACGGTCGCCGGTATCGAC
TTCGCCGACCTGGTCGGCTGGCGTTTCGCTGACGACACCGGCAACCTGGTGCACGACAGC
GGAAAGTTCTTCACCATCGAGGGCCTGTCGGTGGCCGCCGCTCACGGCCCGGTGGCGAGC
TGGTCGCAGCCGATCATCAATCAGCCGGAGACCGGCGTGCTGGGCATCCTGGTCAAGGAG
TTCGACGGGGTGCTGCACTGCCTGATGCAGGCCAAGATGGAGCCGGGCAACTGCAACGTA
TTGCAACTGTCCCCGACCGTGCAGGCCACGCAGAGCAACTACACCGCGGTACACCGCGGC
CGCCCGGTGCCCTATGTGGACATGTTCCTGTCCGCGCCACCGCAGTCGGTGCTCGCCGAC
GTGCTGCAGTCCGAGCAAGGCTCGTGGTTCTACCGCAAACGCAACCGCAACATCGTCGTG
GAGACCACCGCGGACGTCGAGGTGCTGCCCGGCTTCTGCTGGCTGTCACTGGGCCAGGTG
CACGCGCTGCTCACCGAGGACCACCTGGTCAACATGAACACGCGCACTGTGCTGTCCTGC
CTGTCCTTCTCCGGACCGAACCTCGTGGGCGAGCTGGGCTGTGGCGCGTCCGGGTTCCGG
GCGGCCCTGGTGCGGTCGATCGAAACGGCGTGGGGCGCACTGCACACCGACGGCGAGATC
CTCAGCTGGATCACCGACCTGCGGTCCCGCCGCGAGGTCGAGGCCACCCCGATCCCGCTG
TGCGAGGTGCGCGGCTGGCGCCGGCTGCCCGACCGGATCACTCACGACACGGGCGTCTTC
TTCGACGTCCGCGCGGTCGCGGTGGACGGCGGGGGCCGCGAGATCGACAGCTGGACGCAG
CCGGTCATCGCCCCGCACGGGCTCGGTGTCGTGGCGTTCCTCGTCGCCCGGTTCTCCGGG
GTGTTGCACGTACTGGTCGGCGTCCAGGTGGAGCCGGGGCTCCTGGACTTCGTCGAGCTG
GGGCCGACGGTGCAGTGCACGCCGGACAGCTACGAGCACCTGCCGGCCGAGGCCAGGCCG
CCCTTCCTGGACGACGTGCTGGCCGCGCCGGCCGACCGGATCCGGTTCGACACCGAACTC
TCCGAGGAGGGCGGCCGGTTCTTCCACGCCCGTACCCGGCACATGATCGTCGAGGTCGAC
CCGGTGGTGGCGCACGGCGCCGGACCGGCCCGGCACCGGTGGATGACCATGGCCCAGATC
GTCGGGTTGCTACGGCACAGCCACTACGTGAACGTGCAGGCCCGCAGTCTGGTGGCGTGC
CTGCACAGCCTCGTGGCTCGCCTCGATGACGACAGTCCGGTCGCGCCGGGTGGGGGAGCC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005212 NDP-hexose 2,3-dehydratase (Domain)
[37-241] 3.19999999999996e-84 PF03559 [275-481] 1.20000000000001e-77 PF03559
PF03559   Hexose_dehydrat
SignalP No significant hit
TMHMM No significant hit
Conca_00180 Conca_00180   Conca_00200 Conca_00200
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