Conca_00280 : CDS information

Location

Organism	Streptomyces neyagawaensis
Strain	ATCC 27449 (=NBRC 13477)
Entry name	Concanamycin A
Contig	DQ149987
Start / Stop / Direction	98,435 / 99,298 / + [in whole cluster] 98,435 / 99,298 / + [in contig]
Location	98435..99298 [in whole cluster] 98435..99298 [in contig]
Type	CDS
Length	864 bp (287 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative 3-hydroxyacyl-CoA dehydrogenase putative beta-keto-reductase
Product (GenBank)	hydroxybutyryl CoA reductase
Gene
Gene (GenBank)
EC number	1.1.1.35
Keyword	ethylmalonyl-CoA
Note
Note (GenBank)	ORF 19*; ethylmalonate biosynthesis
Reference	ACC Q3S875 PmId [16207901] Organization of the biosynthetic gene cluster for the macrolide concanamycin A in Streptomyces neyagawaensis ATCC 27449. (Microbiology. , 2005) comment ORF19* ethylmalonate biosynthesis (beta-hydroxybutyryl CoA reductase) 配列解析から機能推定。 ORF18とORF19は、module 10のbutyrate extender unitの合成に関与しそう。
Related Reference	ACC P52041 PmId [8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996) comment 472nd(P52041) 43%, 3e-55 Clostridium acetobutylicum_hbd 3-hydroxybutyryl-CoA dehydrogenase(EC 1.1.1.157) BCS (butyryl-CoA synthesis) operon (crt-bcd-etfB-etfA-hbd)のクローニングとシークエンス crt geneの上流にあるプロモーターによって制御されるtranscriptional unitであることを確認 BCS operonをE. coliで大量発現。crotonase, beta-hydroxybutyryl-CoA dehydrogenase activity確認 BCS operonをC. acetobutylicum で発現。crotonase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase activity上昇

Sequence

>putative 3-hydroxyacyl-CoA dehydrogenase [hydroxybutyryl CoA reductase]
MNAIQKVAVVGCGVMGSGIAHVCALAGVDVVVAGSSPASLARGRARLTGSLDRALGKGRI
TDRDHENALARVSYTSDLAELADRQLVIESISEDEQAKRDLFARLDQVLQDPETILASNT
SSLSVLRLARATADGGRVFGVHFFNPVPALPLVELVPTLLTREKTAVRAEEFVTDILGKR
VIRSPDRAGFTVNALLIPYLLAAVRMVEAGHASAETVDDAMTLGCSHPMGPLRLADLIGL
DVVTSIAEALHEEFREPQYAPPPLLRRLVEAGMLGRKTGRGLYDYAP

>putative 3-hydroxyacyl-CoA dehydrogenase [hydroxybutyryl CoA reductase]
ATGAACGCCATCCAGAAGGTCGCAGTCGTGGGCTGCGGCGTCATGGGCTCGGGCATCGCC
CACGTCTGCGCCCTCGCCGGAGTCGACGTCGTGGTCGCGGGGTCCTCGCCCGCATCGCTC
GCCAGGGGCAGGGCACGGCTCACCGGCTCGCTCGACCGGGCGCTGGGCAAGGGCCGGATC
ACCGACCGGGACCACGAGAACGCCCTGGCCCGGGTGTCGTACACCAGTGACCTGGCGGAA
CTGGCCGACCGGCAGCTGGTCATCGAGTCGATCAGCGAGGACGAGCAGGCCAAGCGCGAC
CTGTTCGCCAGGCTGGACCAGGTGCTCCAGGACCCCGAGACCATCCTCGCCTCCAACACC
TCCTCCCTCTCGGTCCTCAGACTCGCCCGGGCCACCGCCGACGGCGGCCGGGTGTTCGGC
GTCCACTTCTTCAACCCGGTGCCCGCACTTCCGCTGGTCGAGCTGGTCCCCACGCTCCTC
ACCCGGGAGAAGACAGCGGTACGGGCCGAGGAATTCGTGACGGACATCCTCGGCAAGCGC
GTCATCCGGTCCCCGGACCGCGCCGGCTTCACGGTCAACGCCCTGCTCATTCCGTACCTC
CTGGCCGCGGTACGCATGGTGGAGGCGGGCCACGCGTCGGCGGAGACGGTGGACGACGCC
ATGACGCTGGGCTGCTCCCACCCGATGGGTCCGCTGCGGCTCGCCGACCTGATCGGGCTC
GACGTGGTCACCTCCATCGCGGAGGCCCTCCACGAGGAGTTCCGCGAGCCCCAGTACGCC
CCGCCACCGCTCCTACGGCGCCTGGTCGAGGCCGGCATGCTGGGCCGCAAGACGGGCAGG
GGCCTCTACGACTACGCCCCTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain) PF00725 3HCDH IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain) PF02737 3HCDH_N IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR013328 Dehydrogenase, multihelical (Domain) G3DSA:1.10.1040.10 Opine_DH IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family) PIRSF000105 HCDH
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative Eukaryota
TMHMM	Transmembrane 1

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