Asco_00110 Asco_00110   Asco_00130 Asco_00130

Asco_00120 : CDS information

close this sectionLocation

Organism
StrainATCC 14891 (=NBRC 13981)
Entry nameAscomycin
Contig
Start / Stop / Direction13,151 / 12,114 / - [in whole cluster]
13,151 / 12,114 / - [in contig]
Locationcomplement(12114..13151) [in whole cluster]
complement(12114..13151) [in contig]
TypeCDS
Length1,038 bp (345 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative lysine cyclodeaminase
Product (GenBank)FkbL
Gene
Gene (GenBank)fkbL
EC number
Keyword
  • L-pipecolate
Note
Note (GenBank)
  • lysine cyclodeaminase
Reference
ACC
PmId
[10863099] The FK520 gene cluster of Streptomyces hygroscopicus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units. (Gene. , 2000)
comment
FK520 gene clusterの報告。
fkbL: lysine cyclodeaminase
Related Reference
ACC
Q54304
NITE
Rapam_00190
PmId
[9473033] Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase. (J Bacteriol. , 1998)
[16536560] Biosynthesis of pipecolic acid by RapL, a lysine cyclodeaminase encoded in the rapamycin gene cluster. (J Am Chem Soc. , 2006)
comment
2nd(Q54304)[Rapam_00190] 79%, 1e-140
Streptomyces hygroscopicus_rapL
Lysine cyclodeaminase

--
[PMID: 9473033](1998)
rapLのmutant作成。L-pipecolateを加えた培地で育ったものはrapamycin産生量が回復。

--
[PMID: 16536560](2006)
RapLをE.coliで過剰発現、精製、isotopically labeled substratesを使ってcyclodeaminase反応の機構的詳細(alpha-amineの減少、alpha-carbonにおけるH原子の保持)を確かめている。

close this sectionSequence

selected fasta
>putative lysine cyclodeaminase [FkbL]
MQTRVLRQRDIKRILSVIGRDVMMDRLISELHDGFAALGRGEFAEAPPRTGFVRGGDIPG
VIEFMPYRVPGVGVTMKTVSYSPHNYPRFRLPTIVGTMSRLDDDSGSLTALADAGVITAM
RTGAVAAVASRLLARSDSATLTLIGAGAQAVTQAHALSRVLPIERILVSDTDPGHAASFA
DRIAFLGLPVKVTDPAEAAAAADVLSTVTSVPSGKGPVLPAEPRLPHLHVNAIGADEPGK
TELPKTLLDDAFICVDHPGQARAEGEYQQLPDRELGPSLAALCAAPDTAADHRDSLSVLD
STGSAFADHIAFDVLLGFADELGLGRKDAIGSTPEDVLDPYSLPW
selected fasta
>putative lysine cyclodeaminase [FkbL]
ATGCAGACCAGGGTCCTGCGCCAACGTGACATCAAGCGGATTCTCTCCGTGATCGGCCGC
GACGTGATGATGGACCGGCTGATCAGCGAGCTGCACGACGGGTTCGCCGCGCTCGGCCGC
GGCGAGTTCGCCGAGGCGCCGCCGCGTACCGGATTCGTCCGCGGCGGCGACATTCCCGGC
GTCATCGAGTTCATGCCGTACCGGGTCCCCGGTGTCGGCGTGACCATGAAGACGGTTTCG
TACAGTCCGCACAACTATCCGCGCTTCCGGCTGCCGACGATCGTCGGCACGATGTCGCGC
CTCGACGACGACAGCGGCAGTCTGACCGCCCTCGCCGACGCAGGCGTGATCACCGCGATG
CGGACCGGCGCGGTCGCCGCCGTCGCGAGCCGGCTGCTGGCCCGGTCCGACAGCGCCACG
CTCACGCTGATCGGCGCGGGCGCACAGGCGGTGACCCAGGCGCACGCGCTCAGCAGGGTC
CTGCCCATCGAGCGGATCCTGGTCAGCGACACCGACCCCGGCCACGCGGCGTCGTTCGCG
GACCGCATCGCGTTCCTCGGACTGCCGGTCAAGGTGACCGATCCGGCGGAGGCCGCCGCC
GCCGCGGATGTCCTGTCCACCGTCACCTCGGTGCCCTCGGGCAAAGGGCCGGTGCTGCCC
GCCGAGCCGCGGCTGCCGCACCTGCATGTGAACGCCATCGGCGCCGACGAGCCGGGCAAG
ACCGAACTGCCGAAGACCCTGCTCGACGACGCGTTCATCTGCGTCGACCATCCGGGGCAG
GCCCGCGCCGAGGGCGAGTACCAGCAACTGCCGGACCGCGAGCTCGGCCCGTCGCTGGCG
GCCCTGTGCGCGGCACCGGACACCGCGGCGGACCACCGGGACAGCCTGAGCGTCCTCGAC
TCGACGGGCAGCGCGTTCGCCGACCACATCGCGTTCGACGTGCTGCTCGGCTTCGCCGAC
GAACTCGGCCTCGGCCGCAAGGACGCGATCGGGTCGACCCCGGAAGACGTCCTCGACCCG
TACTCGCTGCCATGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003462 Ornithine cyclodeaminase/mu-crystallin (Family)
[10-322] 4.40000000000003e-94 PF02423
PF02423   OCD_Mu_crystall
[1-331] 2.49999811956465e-26 PIRSF001439
PIRSF001439   CryM
IPR016040 NAD(P)-binding domain (Domain)
[135-303] 1.6e-41 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Asco_00110 Asco_00110   Asco_00130 Asco_00130
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