Asco_00110 : CDS information

Location

Organism	Streptomyces hygroscopicus subsp. ascomyceticus
Strain	ATCC 14891 (=NBRC 13981)
Entry name	Ascomycin
Contig	AF235504
Start / Stop / Direction	12,093 / 11,245 / - [in whole cluster] 12,093 / 11,245 / - [in contig]
Location	complement(11245..12093) [in whole cluster] complement(11245..12093) [in contig]
Type	CDS
Length	849 bp (282 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative oxidoreductase
Product (GenBank)	FkbK
Gene
Gene (GenBank)	fkbK
EC number
Keyword	methoxymalonyl-ACP
Note
Note (GenBank)	acyl CoA dehydrogenase
Reference	ACC Q9KIE3 PmId [10863099] The FK520 gene cluster of Streptomyces hygroscopicus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units. (Gene. , 2000) [15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004) comment [PMID:10863099](2000) FK520 gene clusterの報告。 fkbK: glyceryl-ACP C3 oxidation fkbGHIJKは、extender unit mehoxymalonyl-ACPの合成に関与する。 fkbKは3-hydroxyacyl-CoA dehydrogenaseにhomologousな酵素をコードし、glyceryl-ACPのC3からH2を取り、-CH2OH → -CHOにする。実験的に確かめられてはいない。 --- [PMID:15179529](2004) S.hygroscopicus由来fkbG-Kを導入することにより、S. fradiaeはmalonyl-CoA, methylmalonyl-CoA, ethylmalonyl-CoAに加えてmethoxymalonyl-ACPもPKS前駆体として利用できるようになることを、midecamycin PKS genes（最後から2番目の縮合ステップでmethoxymalonateを要求）の異種性発現産物で確認している。
Related Reference	ACC Q8KUG1 NITE Ansam_00150 PmId [11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002) comment 13th(Q8KUG1) 62%, 2e-78 Actinosynnema pretiosum subsp. auranticum_asm13 3-hydroxyacyl-CoA dehydrogenase S. lividansで、AT domainsのひとつがmethoxymalonateに特異的なATで置換されているerythromycin PKSのカセットとasm13-17を同時発現すると、予測された位置にmethoxymalonateが取り込まれた6- deoxyerythronolide Bの派生体が産生された。よって、asm13-17はmethoxymalonyl-ACP生合成に十分である。
	ACC Q7BQ74 PmId [16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006) comment 178th(Q7BQ74) 35%, 1e-44 Bacillus cereus_zmaG ZmaG ZmaGは、珍しいPKS伸長units, hydroxymalonyl-ACP and aminomalonyl-ACPの生合成に共通して関連する。 ZmaE or ZmaIと共に働く。 ZmaI: eryl-ZmaH(ACP)→aminomalonyl-ZmaH(ACP) ZmaE: glyceryl-ZmaD(ACP)→hydroxymalonyl-ZmaD(ACP)
	ACC Q9ADL9 NITE Soraph_00040 PmId [12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002) [15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004) comment Blast 204th, id42%, 5e-44 Sorangium cellulosum_sorD Beta-hydroxybutyryl-CoA dehydrogenase --- [PMID: 12039053](2002) Soraphen A biosynthetic gene clusterの報告。 SorD(295aa): beta-Hydroxybutyryl-CoA dehydrogenase Module 3,7で取り込まれるMethoxymalonyl-CoAを合成する。本ORFはCH2OHをCHOにする。 --- [PMID: 15289572](2004) sorC破壊株はsoraphen A非産生。 S. lividansでのsor genes異種性発現。 SorABRCDFE株 + cinnamate SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate の組み合わせでSoraphen A産生。 SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.

Sequence

>putative oxidoreductase [FkbK]
MDHQLIVLGAGVMGTGITTLALRHGVPVTLVDTSEEILTAARARIATELRTAQLVAGPAE
ADPGVLRTATSALPAIGTATAVIEAVVEDAEVKAEVLATVSAAAPGIPVITNTSSIPVDE
LADSVARPEDLLGTHFMNPSYLIPTVEVIRGPRTGEKAMAAAGDLLAALRRKPIVVRDAP
GFVTSRLLHPMINDAARIVQEGTASVEDVDALMTGCLGHPTGPLRTADLIGIDNLVDALW
VLHKRTGDERSRPCDLLLGKVREGHHGRKTGRGFYEYEKVFS

>putative oxidoreductase [FkbK]
GTGGACCACCAGCTGATCGTGCTCGGCGCGGGAGTGATGGGCACCGGCATCACCACGCTC
GCGCTGCGCCACGGCGTCCCGGTGACACTCGTCGACACGTCCGAGGAGATCCTTACGGCG
GCGCGGGCGCGGATCGCCACCGAACTGCGGACGGCACAACTCGTGGCCGGGCCCGCCGAG
GCCGACCCCGGTGTGCTGCGGACGGCCACGTCCGCGCTGCCCGCGATCGGTACGGCCACC
GCGGTGATCGAGGCCGTCGTCGAGGACGCCGAGGTCAAGGCGGAGGTACTCGCCACGGTC
TCGGCAGCGGCGCCCGGTATCCCGGTCATCACGAACACGTCGTCGATCCCGGTTGACGAA
CTGGCCGACTCCGTGGCCCGGCCCGAGGACCTGCTCGGCACGCACTTCATGAACCCGTCG
TACCTGATCCCGACGGTCGAGGTGATCCGCGGACCCCGGACCGGTGAGAAGGCCATGGCC
GCCGCCGGGGACCTGCTCGCGGCGCTGCGGCGCAAGCCGATCGTCGTCCGGGACGCGCCC
GGCTTCGTCACGAGCCGGCTGCTCCACCCGATGATCAACGACGCGGCGCGGATCGTGCAG
GAGGGCACCGCGTCCGTCGAGGACGTCGACGCGCTCATGACGGGGTGCCTCGGCCATCCG
ACCGGGCCGCTGCGCACCGCGGACCTGATCGGCATCGACAACCTCGTCGACGCGCTGTGG
GTGCTGCACAAACGCACCGGCGACGAGCGCAGCCGCCCCTGTGACCTGCTGCTGGGCAAG
GTCCGCGAGGGACACCACGGCCGGAAGACCGGCCGGGGCTTCTACGAATACGAGAAGGTG
TTCTCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain) PF00725 3HCDH IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain) PF02737 3HCDH_N IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR013328 Dehydrogenase, multihelical (Domain) G3DSA:1.10.1040.10 Opine_DH IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family) PIRSF000105 HCDH
SignalP	No significant hit
TMHMM	No significant hit

Asco_00100 Asco_00120