Asco_00180 : CDS information

Location

Organism	Streptomyces hygroscopicus subsp. ascomyceticus
Strain	ATCC 14891 (=NBRC 13981)
Entry name	Ascomycin
Contig	AF235504
Start / Stop / Direction	71,460 / 72,626 / + [in whole cluster] 71,460 / 72,626 / + [in contig]
Location	71460..72626 [in whole cluster] 71460..72626 [in contig]
Type	CDS
Length	1,167 bp (388 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	FkbD
Gene
Gene (GenBank)	fkbD
EC number
Keyword	C-9 hydroxylate
Note
Note (GenBank)	C9 hydroxylase
Reference	ACC Q9KID6 PmId [10863099] The FK520 gene cluster of Streptomyces hygroscopicus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units. (Gene. , 2000) comment FK520 gene clusterの報告。 fkbD: P450 C9-hydroxylase PKSによって合成された中間体はFkbPによって縮合されてPKS complexから放出されると、alpha-ketoamide functionを得るためにC9がFkbDによってhydroxylateされ、FkbOによって酸化されると提唱。
Related Reference	ACC P77977 PmId [8752344] Characterization of methyltransferase and hydroxylase genes involved in the biosynthesis of the immunosuppressants FK506 and FK520. (J Bacteriol. , 1996) comment 2nd(P77977) 89%, 0.0 Streptomyces sp.(MA6858)_fkbD Cyt P-450-9-deoxo-FK506 hydroxylase Disruption mutantを作製して実験している。 fkbDの破壊は下流にあるfkbMの合成にpolar effectを及ぼし、その結果9-deoxo-31-O-demethyl-FK506の形成をもたらす。これはFkbDをcytochrome P-450 9-deoxo-FK506 hydroxylaseとして確立し、それはFK506 and FK520 macrolactone ringのC-9におけるhydroxylationを担う。

Sequence

>putative cytochrome P450 [FkbD]
MSTDTHEGTPPAGRCPFAIQDGHRAILESGTVGSFDLFGVKHWLVAAAEDVKLVTNDPRF
SSAAPSEMLPDRRPGWFSGMDSPEHNRYRQKIAGDFTLRAARKREDFVAEAADACLDDIE
AAGPGTDLIPGYAKRLPSLVINALYGLTPEEGAVLEARMRDITGSADLDSVKTLTDDFFG
HALRLVRAKRDERGEDLLHRLASADDGEISLSDDEATGVFATLLFAGHDSVQQMVGYCLY
ALLSHPEQQAALRARPELVDNAVEEMLRFLPVNQMGVPRVCVEDVDVRGVRIRAGDNVIP
LYSTANRDPEVFPQPDTFDVTRPLEGNFAFGHGIHKCPGQHIARVLIKVACLRLFERFPD
VRLAGDVPMNEGLGLFSPAELRVTWGAA

>putative cytochrome P450 [FkbD]
ATGAGCACCGATACGCACGAGGGAACGCCGCCCGCCGGCCGCTGCCCATTCGCGATCCAG
GACGGTCACCGCGCCATCCTGGAGAGCGGCACGGTGGGTTCGTTCGACCTGTTCGGCGTC
AAGCACTGGCTGGTCGCCGCCGCCGAGGACGTCAAGCTGGTCACCAACGATCCGCGGTTC
AGCTCGGCCGCGCCGTCCGAGATGCTGCCCGACCGGCGGCCCGGCTGGTTCTCCGGGATG
GACTCACCGGAGCACAACCGCTACCGGCAGAAGATCGCGGGGGACTTCACACTGCGCGCG
GCGCGCAAGCGGGAGGACTTCGTCGCCGAGGCCGCCGACGCCTGCCTGGACGACATCGAG
GCCGCGGGACCCGGCACCGACCTCATCCCCGGGTACGCCAAGCGGCTGCCCTCCCTCGTC
ATCAACGCGCTGTACGGGCTCACCCCTGAGGAGGGGGCCGTGCTGGAGGCACGGATGCGC
GACATCACCGGCTCGGCCGATCTGGACAGCGTCAAGACGCTGACCGACGACTTCTTCGGG
CACGCGCTGCGGCTGGTCCGCGCGAAGCGTGACGAGCGGGGCGAGGACCTGCTGCACCGG
CTGGCCTCGGCCGACGACGGCGAGATCTCGCTCAGCGACGACGAGGCGACGGGCGTGTTC
GCGACGCTGCTGTTCGCCGGCCACGACTCGGTGCAGCAGATGGTCGGCTACTGCCTCTAC
GCACTGCTCAGCCACCCCGAGCAGCAGGCGGCGCTGCGCGCGCGCCCGGAGCTGGTCGAC
AACGCGGTCGAGGAGATGCTCCGTTTCCTGCCCGTCAACCAGATGGGCGTACCGCGCGTC
TGTGTCGAGGACGTCGATGTGCGGGGCGTGCGCATCCGTGCGGGCGACAACGTGATCCCG
CTCTACTCGACGGCCAACCGCGACCCCGAGGTGTTCCCGCAGCCCGACACCTTCGATGTG
ACGCGCCCGCTGGAGGGCAACTTCGCGTTCGGCCACGGCATTCACAAGTGTCCCGGCCAG
CACATCGCCCGGGTGCTCATCAAGGTCGCCTGCCTGCGGTTGTTCGAGCGTTTCCCGGAC
GTCCGGCTGGCCGGCGACGTGCCGATGAACGAGGGGCTCGGGCTGTTCAGCCCGGCCGAG
CTGCGGGTCACCTGGGGGGCGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Asco_00170 Asco_00190