Asco_00190 : CDS information

Location

Organism	Streptomyces hygroscopicus subsp. ascomyceticus
Strain	ATCC 14891 (=NBRC 13981)
Entry name	Ascomycin
Contig	AF235504
Start / Stop / Direction	72,623 / 73,405 / + [in whole cluster] 72,623 / 73,405 / + [in contig]
Location	72623..73405 [in whole cluster] 72623..73405 [in contig]
Type	CDS
Length	783 bp (260 aa)

Annotation

Category	3.2 modification methylation
Product	C-31 O-methyltransferase
Product (GenBank)	FkbM
Gene
Gene (GenBank)	fkbM
EC number	2.1.1.-
Keyword
Note
Note (GenBank)	31-O-methyltransferase
Reference	ACC Q9KID5 PmId [10863099] The FK520 gene cluster of Streptomyces hygroscopicus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units. (Gene. , 2000) [8752344] Characterization of methyltransferase and hydroxylase genes involved in the biosynthesis of the immunosuppressants FK506 and FK520. (J Bacteriol. , 1996) comment [PMID: 10863099](2000) FK520 gene clusterの報告。 fkbM: 31-O-methyl transferase --- [PMID: 8752344](1996) Disruption mutantを作製して実験している。

Sequence

>C-31 O-methyltransferase [FkbM]
MSHPVETLRLPNGTTVAHINAGEAQFLYREIFTQRCYLRHGVDLRPGDVVFDVGANIGMF
TLFAHLECPGVTVHAFEPAPVPFAALRANVTRHGIPGQADQCAVSDSSGTRKMTFYPDAT
LMSGFHADAAARTELLRTLGLNGGYTAEDVDTMLAQLPDVSEEIETPVVRLSDVIAERGI
EAIGLLKVDVEKSERQVFAGLEDTDWPRIRQVVAEVHDIDGALEEVVTLLRGHGFTVVAE
QEPLFAGTGIHQVAARRVAG

>C-31 O-methyltransferase [FkbM]
ATGAGTCACCCGGTGGAGACGTTGCGGTTGCCGAACGGGACGACGGTCGCGCACATCAAC
GCGGGCGAGGCGCAGTTCCTCTACCGGGAGATCTTCACCCAGCGCTGCTACCTGCGCCAC
GGTGTCGACCTGCGCCCGGGGGACGTGGTGTTCGACGTCGGCGCGAACATCGGCATGTTC
ACGCTTTTCGCGCATCTGGAGTGTCCTGGTGTGACCGTGCACGCCTTCGAGCCCGCGCCC
GTGCCGTTCGCGGCGCTGCGGGCGAACGTGACGCGGCACGGCATCCCGGGCCAGGCGGAC
CAGTGCGCGGTCTCCGACAGCTCCGGCACCCGGAAGATGACCTTCTATCCCGACGCCACG
CTGATGTCCGGTTTCCACGCGGATGCCGCGGCCCGGACGGAGCTGTTGCGCACGCTCGGC
CTCAACGGCGGCTACACCGCCGAGGACGTCGACACCATGCTCGCGCAACTGCCCGACGTC
AGCGAGGAGATCGAAACCCCTGTGGTCCGGCTCTCCGACGTCATCGCGGAGCGCGGTATC
GAGGCCATCGGCCTGCTGAAGGTCGACGTGGAGAAGAGCGAACGGCAGGTCTTCGCCGGC
CTCGAGGACACCGACTGGCCCCGTATCCGCCAGGTCGTCGCGGAGGTCCACGACATCGAC
GGCGCGCTCGAGGAGGTCGTCACGCTGCTCCGCGGCCATGGCTTCACCGTGGTCGCCGAG
CAGGAACCGCTGTTCGCCGGCACGGGCATCCACCAGGTCGCCGCGCGGCGGGTGGCCGGC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006342 Methyltransferase FkbM (Domain) TIGR01444 FkbM_fam PF05050 Methyltransf_21
SignalP	No significant hit
TMHMM	No significant hit

Asco_00180 Asco_00200