Asco_00200 Asco_00200   Asco_00220 Asco_00220

Asco_00210 : CDS information

close this sectionLocation

Organism
StrainATCC 14891 (=NBRC 13981)
Entry nameAscomycin
Contig
Start / Stop / Direction77,078 / 76,335 / - [in whole cluster]
77,078 / 76,335 / - [in contig]
Locationcomplement(76335..77078) [in whole cluster]
complement(76335..77078) [in contig]
TypeCDS
Length744 bp (247 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative type II thioesterase
Product (GenBank)FkbQ
Gene
Gene (GenBank)fkbQ
EC number
Keyword
  • type II thioesterase
Note
Note (GenBank)
  • thioesterase II
Reference
ACC
PmId
[10863099] The FK520 gene cluster of Streptomyces hygroscopicus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units. (Gene. , 2000)
comment
FK520 gene clusterの報告。
fkbQ: type II thioesterase

fkbQはtypeII thioesterases(TE-II)のhomoogueで、PKSで形成された異常産物のエディタとして働くことが提案されている。rapamycin clusterはTE-II geneを含んでおらず、FK520生合成のためのTE-IIの重要性は判定されていない。
Related Reference
ACC
Q7BUF9
NITE
Rifam_00540
PmId
[19103602] Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway. (J Biol Chem. , 2009)
comment
15th(Q7BUF9) 50%, 8e-59
Amycolatopsis mediterranei_rifR
[Rifam_00540]type II thioesterase

[PMID: 19103602](2009)
RifRは、acyl-CoAsよりacyl carrier domainのphosphopantetheinyl armからのacyl unitsをhydrolysisをする(Steady-state kinetics)。
helical lidの運動が、RifR active siteへの基質のアクセスを調節する(立体構造解析)。
RifRはCoA基質よりACP基質への反応を好む。これはtype II thioesteraseがcarrier domainsから異常unitsを除くようなhousekeeping functionsをすると考えられていることに一致する。

close this sectionSequence

selected fasta
>putative type II thioesterase [FkbQ]
MSSELWLRQFFPTPEAPVRVVLLPHAGGSATFYLPLARALAPVADVYAVQYPGRQDRRDE
EAVRDLRVLAGLVAEALKPGLDRPLVLFGHSMGATLAFELAQRLPVAHLIVSGRRAPSQP
RADQDHLASDDELLAAVIGLGGTSADVMADDEMRALILAPLRADYYAAETYRWEPAPPLE
TPITVCIGDDDPKTTVEQAHAWAGHTTAPTTVEVFPGGHFYLVDHQERVAELVAQGVRRV
APGAVGS
selected fasta
>putative type II thioesterase [FkbQ]
GTGAGCTCGGAACTCTGGCTGCGGCAGTTCTTCCCCACGCCCGAGGCCCCGGTACGGGTG
GTGCTGCTGCCCCACGCCGGCGGATCGGCGACGTTCTACCTTCCGCTGGCCCGTGCGCTC
GCCCCGGTGGCGGACGTGTACGCCGTGCAGTACCCCGGCCGCCAGGACCGCCGCGACGAG
GAGGCGGTGCGCGACCTGCGTGTTCTCGCCGGCCTCGTGGCCGAGGCGTTGAAGCCGGGG
CTGGACCGTCCGCTGGTGCTGTTCGGCCACAGCATGGGCGCGACACTCGCGTTCGAGCTG
GCCCAGCGGCTCCCGGTGGCGCACCTGATCGTCTCGGGCCGGCGGGCGCCGTCGCAGCCG
CGCGCCGACCAGGACCACCTCGCGTCCGACGACGAGCTGCTGGCGGCGGTCATCGGCCTC
GGCGGGACGAGCGCCGATGTGATGGCGGACGACGAAATGCGCGCGCTGATCCTGGCGCCG
TTGCGGGCGGACTACTACGCGGCGGAGACGTACCGGTGGGAGCCCGCGCCGCCGCTGGAG
ACGCCGATCACGGTGTGCATCGGTGACGACGATCCGAAGACGACGGTGGAGCAGGCCCAC
GCCTGGGCCGGGCACACGACCGCGCCGACCACGGTGGAGGTCTTCCCCGGCGGGCACTTC
TACCTCGTCGACCACCAGGAGCGGGTCGCCGAGCTGGTGGCCCAGGGGGTACGGCGGGTG
GCGCCGGGGGCGGTCGGCTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[19-235] 6.59999999999996e-49 PF00975
PF00975   Thioesterase
SignalP No significant hit
TMHMM No significant hit
Asco_00200 Asco_00200   Asco_00220 Asco_00220
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