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Asco_00220 : CDS information

close this sectionLocation

Organism
StrainATCC 14891 (=NBRC 13981)
Entry nameAscomycin
Contig
Start / Stop / Direction77,497 / 77,075 / - [in whole cluster]
77,497 / 77,075 / - [in contig]
Locationcomplement(77075..77497) [in whole cluster]
complement(77075..77497) [in contig]
TypeCDS
Length423 bp (140 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative crotonyl-CoA reductase(fragment)
Product (GenBank)FkbS
Gene
Gene (GenBank)fkbS
EC number
Keyword
  • ethylmalonyl-CoA
Note
Note (GenBank)
  • crotonyl CoA reductase
Reference
ACC
PmId
[10863099] The FK520 gene cluster of Streptomyces hygroscopicus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units. (Gene. , 2000)
comment
FK520 gene clusterの報告。
fkbS: crotonyl-CoA reductase

fkbSはcrotonyl-CoA reductaseをコードする(相同性から)。
crotonyl-CoA → butyryl-CoA
この反応はethylmalonyl-CoAの作成に関与する。

fkbSはstrain14891でのbutyryl-CoA生合成に必要かもしれないが、他のpolyketide産生株でのbutyryl-CoA生合成はclusterの外にある遺伝子によってコードされるcrotonyl-CoA reductaseにもっと依存的であるらしい。
Related Reference
ACC
Q9RNU6
PmId
[10542184] Role of crotonyl coenzyme A reductase in determining the ratio of polyketides monensin A and monensin B produced by Streptomyces cinnamonensis. (J Bacteriol. , 1999)
comment
8th(Q9RNU6) 76%, 1e-55, [322-451]/453aaのC末hit
Streptomyces cinnamonensis_ccr
Crotonyl-CoA reductase

S. cinnamonensis_ccr、そのS. cinnamonensisで異種性発現させたS. collinus_ccr、ccr-disrupted mutantなどでCCR activity測定あり。
monensin A/monensin Bの比率は、CCR活性のレベルと、CCRの基質であるcrotonyl-CoAの両方に依存する。

close this sectionSequence

selected fasta
>putative crotonyl-CoA reductase(fragment) [FkbS]
MFEHPGRATFGASVYVTRRGGTIVTCASTSGYEHAFDNRYLWMSVKRIIGTHFANYREAW
EANRLICKGMVHPTLSVTYPLDDVGSAVRDVHRNVHNGKVGVLCLAPEEGWGVTDPEMRE
KHLAEITRFRAPQVAAEGVR
selected fasta
>putative crotonyl-CoA reductase(fragment) [FkbS]
GTGTTCGAGCACCCGGGGCGCGCGACGTTCGGCGCGAGTGTCTACGTGACACGTCGGGGT
GGCACGATCGTCACCTGCGCGTCAACCTCGGGATACGAGCACGCCTTTGACAATCGGTAC
CTGTGGATGTCCGTGAAGCGGATCATCGGTACCCACTTCGCGAACTATCGTGAGGCGTGG
GAGGCGAACCGCCTGATCTGCAAGGGGATGGTGCACCCGACGCTGTCGGTGACCTACCCG
CTCGACGACGTCGGGTCGGCGGTGCGCGATGTGCATCGCAATGTGCACAACGGCAAAGTC
GGCGTCCTCTGTCTGGCGCCGGAGGAGGGATGGGGCGTGACCGACCCGGAGATGCGGGAG
AAGCACTTGGCCGAGATCACCCGGTTCCGGGCTCCCCAGGTCGCGGCCGAGGGGGTGCGG
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013149 Alcohol dehydrogenase, C-terminal (Domain)
[2-66] 5.6e-16 PF00107
PF00107   ADH_zinc_N
IPR016040 NAD(P)-binding domain (Domain)
[2-56] 4.6e-05 G3DSA:3.40.50.720
G3DSA:3.40.50.720   no description
SignalP
[1-35] 0.263 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
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