Aureo_00010 : CDS information

Location

Organism	Streptomyces thioluteus
Strain	HKI-227
Entry name	Aureothin
Contig	AJ575648
Start / Stop / Direction	2,154 / 2,969 / + [in whole cluster] 2,154 / 2,969 / + [in contig]
Location	2154..2969 [in whole cluster] 2154..2969 [in contig]
Type	CDS
Length	816 bp (271 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative transcriptional regulator
Product (GenBank)	AurD protein
Gene
Gene (GenBank)	aurD
EC number
Keyword	SARP family
Note
Note (GenBank)
Reference	ACC Q70KI1 PmId [14700630] Iteration as programmed event during polyketide assembly; molecular analysis of the aureothin biosynthesis gene cluster. (Chem Biol. , 2003) comment aureothin生合成gene clusterの同定論文。配列解析からの機能推定。 AurD: transcriptional activator
Related Reference	ACC P46106 NITE Actino_00160 PmId [1878971] The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces. (Cell. , 1991) [7683365] Stationary-phase production of the antibiotic actinorhodin in Streptomyces coelicolor A3(2) is transcriptionally regulated. (Mol Microbiol. , 1993) comment 227th(P46106) 35%, 7e-24 Streptomyces coelicolor_actII-4 Probable actinorhodin operon activatory protein [Actino_00160]transcriptional activator --- [PMID: 1878971](1991） actII-ORF4導入でactinorhodin産生増加。 actII-ORF4破壊でactinorhodin非産生。 actII mutant strainでは、act生合成遺伝子転写物がなくなったり減ったりした(unpublished)。 actIII geneの転写にはactII領域が同時に存在することが必要(Hallam et al. 1988)。 actII-ORF4にはTTA codonが含まれているため、翻訳にはBldAが必要。 --- [PMID: 7683365](1993) abstract 対数期→静止期でactII-ORF4転写(↑) 同時に、actIII, actVI-ORF1転写(↑)、actinorhodin産生(↑) 対数期でmulticopy plasmidによってactII-ORF4を増やすと、 →actII-ORF4 mRNA(↑) →actIII転写、actinorhodin産生をもたらす

Sequence

>putative transcriptional regulator [AurD protein]
MVNAVVKLPSDLSFNILGPVAVSSTEGPLPLGPARQRAVLSALLLAPGQVVGAERLTEAI
WPAGPPGNAVASLHSYVSHLRRQLEPDCPPWGRNRILRREPQGYLLAVEAERVDARRFEG
LLREGRQLLREGRYPEASQVLRACLELWRGSPYAELGDYEPAVCEAARLEELRLATLEAL
WEAELAWGHDYVTAIAELAAVSARFPTRERLGWLLMVALCEVDRQAEAVAVYNRIRKALA
QELGVDPGRELRSLFARILRDESVGRACLQS

>putative transcriptional regulator [AurD protein]
CTGGTGAATGCCGTTGTGAAACTCCCGTCCGACTTATCCTTCAACATCCTCGGGCCGGTG
GCGGTGTCGTCGACGGAGGGCCCGCTGCCGCTGGGCCCGGCGCGTCAACGCGCCGTGCTG
TCGGCCCTGTTGCTGGCGCCGGGGCAGGTGGTCGGGGCGGAGCGGCTCACGGAGGCGATA
TGGCCGGCGGGCCCTCCCGGCAACGCCGTCGCAAGTCTCCACAGCTACGTCTCCCACCTC
CGCCGCCAACTGGAGCCCGACTGCCCGCCCTGGGGCCGCAACCGGATCCTCCGCCGGGAG
CCGCAGGGCTATCTCCTCGCCGTCGAGGCGGAACGGGTGGACGCCCGGCGCTTCGAGGGT
CTGCTGCGCGAGGGGCGCCAACTGCTGCGGGAGGGCCGCTACCCGGAGGCGAGCCAGGTC
CTCCGGGCATGCCTGGAGCTGTGGCGCGGCTCGCCGTACGCCGAACTCGGCGACTACGAG
CCCGCCGTGTGCGAGGCGGCGCGCCTGGAGGAGCTGCGCCTGGCGACGCTGGAGGCCCTC
TGGGAGGCCGAGCTGGCCTGGGGGCACGACTACGTCACGGCCATCGCCGAACTGGCCGCG
GTGAGCGCGCGGTTCCCCACCCGCGAACGGCTGGGGTGGCTGCTGATGGTCGCCCTGTGC
GAGGTCGACCGGCAGGCGGAGGCGGTGGCGGTGTACAACCGCATCCGCAAGGCGCTGGCC
CAGGAACTGGGCGTGGACCCGGGCCGGGAGCTGCGGTCGCTGTTCGCGCGGATCCTGCGT
GACGAGTCCGTCGGGCGGGCCTGCCTGCAGAGTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001867 Signal transduction response regulator, C-terminal (Domain) PF00486 Trans_reg_C SM00862 Trans_reg_C IPR005158 Bacterial transcriptional activator domain (Domain) PF03704 BTAD SM01043 BTAD IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	No significant hit
TMHMM	No significant hit

first Aureo_00020