Aureo_00010 Aureo_00010   Aureo_00030 Aureo_00030

Aureo_00020 : CDS information

close this sectionLocation

Organism
StrainHKI-227
Entry nameAureothin
Contig
Start / Stop / Direction4,546 / 3,029 / - [in whole cluster]
4,546 / 3,029 / - [in contig]
Locationcomplement(3029..4546) [in whole cluster]
complement(3029..4546) [in contig]
TypeCDS
Length1,518 bp (505 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative carboxylic acid--CoA ligase
putative acyl-CoA synthetase
Product (GenBank)putative acyl-coA ligase
Gene
Gene (GenBank)aurE
EC number6.2.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[14700630] Iteration as programmed event during polyketide assembly; molecular analysis of the aureothin biosynthesis gene cluster. (Chem Biol. , 2003)
[23606628] Multifactorial control of iteration events in a modular polyketide assembly line. (Angew Chem Int Ed Engl. , 2013)
comment
[PMID:14700630](2003)
aureothin生合成gene clusterの同定論文。

配列解析からの機能推定。
AurE: Acyl-CoA ligase

aur PKS primer p-nitrobenzoate(PNBA)をCoA thioesterとして活性化すると提唱。

---
[PMID:23606628](2013)
module1での反復過程について調査している。

ΔaurE mutantはaureothin非産生。発現plasmidによるaurEでの相補で産生は回復した。
また、PNBA-CoAの代理としてPNBA-SNACを補充してもaureothin生合成を回復した。

よって、AurEはstarter unit活性化に関連するが、PKS primingには関与しない。
Related Reference
ACC
Q9EY88
PmId
[11152072] Identification of Amycolatopsis sp. strain HR167 genes, involved in the bioconversion of ferulic acid to vanillin. (Appl Microbiol Biotechnol. , 2000)
comment
158th(Q9EY88) 36%, 8e-66
Amycolatopsis sp. HR167_fcs
Feruloyl-CoA synthetase

Amycolatopsis sp. strain HR167からech (encoding enoyl-CoA hydratase/aldolase), fcs (encoding an unusual feruloyl-CoA synthetase)を含む4kb断片をクローニングしてシークエンス。
この断片をE. coliに導入するとferulic acid → vanillinへtransformできるようになり、feruloyl-CoA synthetase活性、enoyl-CoA hydratase/aldolase活性が検出された。

close this sectionSequence

selected fasta
>putative carboxylic acid--CoA ligase [putative acyl-coA ligase]
MLVPLTTADFLDRAEATAAARTALVDEPHQPAAALPTSTYGETLRRVRAWQAGLDALGID
EGERVAVVSPNSARLLELLYAVTASGRICVPVNYRLTPEEISYILRQCDASVVFVDPDVE
AVLGSVDVPKRFVLGEQTETEVMRFDVAPRPWSRPDENAVATLNYTSGTSARPKGVALTH
RSIWLNAMTFGVHARIWEGDVYLHTLPTFHCNGWGVPFLLAGLGAKQVLIRRIDGAEILR
RVQEHGVTLAFGAPAVWDAVLQAARDWPGEIPGRGRVRVVCAGAPVSAQLVSRIENELGW
EFLQVYGLTETTLLTFNRRLPDAPGDGGLTRAGAPALGVRLRTGERGEVLARSNMVLDSY
WRDEQANEAALEDGWFRTGDVGAFDDEGHLALFDRVKDVIVTGGESVSSVEVEDCLQGHP
SIAEVAVIGVPDERWGETVKAVVVPADGARVTEEEVITYCKQQLARYKAPTSVDFRDALP
RTSNGKIQKGRLREPYWAGHDRRIH
selected fasta
>putative carboxylic acid--CoA ligase [putative acyl-coA ligase]
ATGCTCGTTCCGCTCACCACGGCCGATTTCCTCGACCGCGCCGAGGCAACCGCCGCCGCC
CGCACCGCACTTGTCGACGAGCCGCACCAGCCGGCCGCCGCGCTGCCCACCAGTACGTAC
GGGGAGACGCTGCGGCGCGTCCGCGCCTGGCAGGCCGGCCTGGACGCGCTGGGGATCGAC
GAGGGCGAACGGGTCGCCGTCGTCAGCCCCAACTCGGCCCGGCTGCTGGAGCTCCTCTAC
GCGGTCACGGCGAGCGGACGCATCTGCGTCCCGGTGAACTACCGGCTCACGCCCGAGGAG
ATCTCCTACATCCTGCGCCAGTGCGACGCGTCCGTCGTCTTCGTCGACCCCGACGTCGAG
GCGGTCCTGGGCTCGGTCGACGTGCCGAAGCGGTTCGTCCTCGGCGAGCAGACCGAGACC
GAGGTCATGCGGTTCGACGTCGCCCCCCGCCCCTGGTCACGGCCCGACGAGAACGCGGTC
GCGACCCTCAACTACACCTCGGGCACCTCCGCCCGCCCCAAGGGCGTCGCGCTCACCCAC
CGCAGCATCTGGCTCAACGCGATGACCTTCGGCGTCCACGCCCGGATCTGGGAGGGGGAC
GTCTACCTCCACACCCTGCCCACGTTCCACTGCAACGGCTGGGGCGTGCCGTTCCTCCTC
GCCGGGCTCGGCGCCAAGCAGGTCCTGATCCGCCGGATCGACGGCGCGGAGATCCTGCGC
CGCGTCCAGGAGCACGGCGTGACGCTGGCCTTCGGCGCCCCCGCCGTGTGGGACGCGGTG
CTCCAGGCCGCCCGCGACTGGCCGGGCGAGATCCCCGGCCGCGGCCGGGTCCGCGTGGTC
TGCGCGGGAGCCCCCGTCAGCGCACAGCTGGTCTCCCGCATAGAGAACGAACTGGGCTGG
GAGTTCCTCCAGGTCTACGGCCTCACCGAGACGACGCTGCTCACCTTCAACCGGCGCCTG
CCGGACGCCCCCGGGGACGGCGGGCTGACGAGGGCCGGCGCCCCCGCCCTGGGCGTGCGG
CTGCGCACCGGGGAGCGCGGCGAGGTGCTGGCGCGCTCCAACATGGTGCTCGACAGCTAC
TGGCGCGACGAGCAGGCCAATGAAGCCGCGCTGGAGGACGGCTGGTTCCGCACCGGCGAC
GTGGGCGCCTTCGACGACGAGGGGCACCTGGCCCTCTTCGACCGGGTGAAGGACGTCATC
GTCACCGGCGGCGAGAGCGTGTCCTCGGTGGAGGTCGAGGACTGCCTCCAGGGCCACCCC
TCCATCGCCGAAGTGGCCGTCATCGGCGTCCCGGACGAGCGGTGGGGCGAGACCGTCAAG
GCGGTCGTCGTCCCCGCCGACGGGGCCCGGGTCACCGAGGAAGAGGTCATCACCTACTGC
AAACAGCAGCTGGCCCGGTACAAGGCGCCCACGTCCGTGGACTTCCGGGACGCCCTGCCC
CGGACCAGCAACGGCAAGATCCAGAAGGGCCGGCTCAGGGAGCCCTACTGGGCCGGCCAC
GACCGGCGGATCCACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[39-427] 9.10000000000013e-84 PF00501
PF00501   AMP-binding
IPR025110 Domain of unknown function DUF4009 (Domain)
[467-499] 2.6e-07 PF13193
PF13193   DUF4009
SignalP No significant hit
TMHMM No significant hit
Aureo_00010 Aureo_00010   Aureo_00030 Aureo_00030
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