Borre_00130 Borre_00130   Borre_00150 Borre_00150

Borre_00140 : CDS information

close this sectionLocation

Organism
StrainTü4055
Entry nameBorrelidin
Contig
Start / Stop / Direction15,659 / 13,608 / - [in whole cluster]
15,659 / 13,608 / - [in contig]
Locationcomplement(13608..15659) [in whole cluster]
complement(13608..15659) [in contig]
TypeCDS
Length2,052 bp (683 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
ProductCoA-transferase family protein
Product (GenBank)putative dehydratase
Gene
Gene (GenBank)borH
EC number
Keyword
  • trans-1,2-CPDA
Note
Note (GenBank)
Reference
ACC
PmId
[15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004)
comment
Borrelidin生合成gene clusterのクローニング。
borH: starter unit biosynthesis
closest similar to hypo

borH productは、starter unit(trans-1,2-CPDA)生合成において定義される役割を示唆するタンパクとの明らかな類似が見られない。

borH insertional inactivation mutantはborrelidin産生が1/5以下に減る。
このmutant株にtrans-1,2-CPDAを加えるとborrelidin産生はほぼ回復。

以上から、BorHはtrans-1,2-CPDAの生合成によく関連しているかもしれないが、その役割まではわからない。
Family/Domain
FD
IPR003673
comment
[IPR003673] CoA-transferase family III (Family)

close this sectionSequence

selected fasta
>CoA-transferase family protein [putative dehydratase]
MRGARENSMTRAGPLEGIAVLMAGRSTPAALLGRLLADLGARVVTLCRSPDHGGPFERWL
HSAAQSASGWDQASRLLQTADVLVCDAEGDERLAALGLGAPELPHRSPELVAVRLSAFGL
TGPLRDAPATERTLQALAGLTSATGTEGEPSVLSVVGLASRTAALSGLIAVVAGLIGRER
GGGGDYLDIAEFDSLFTLTGTLLPSVALAGRPPRRTGNRHGMAAPWNSYTCQDAPVVICT
MGEPIWHRLTAVLGRRDLPDDPRFADTAARVRNADELDEILGKWTAGQRAVDVVTALRAA
GIPCAQVAAPEEVRDGAAARRRGLVTDPSGTPGSPLRSLIPAVTDGPMPRQGGLWEPIAR
GTPPLRGVRLLEVGSYTAGPHAGRLLAQLGADVLKVEPPHGEGSRRLAQQVAGVGYLYYV
NNAGKRSCRLDLADAEDRAGFERLLAGCDIVLTNLAADTLTAQGLAPDQILSRHGVVHCT
VTGHGLAAADRSVDTVIQAESGIMRLVGGPGAGLRTPVSSADVLGAYLAAAAAVVSTYVR
LRTEHGCAADVALFDSAVWLTQDRWFTAPPARAPHLVRAADGTVLVDAEGPPPRAEGPVA
AVLDAAAAVGVPAAPLHDLTRAVRHPQVLARRMAVARDCAGTTVLITGNHLRSLLREDPP
PTCAPVDQNDPVWLQPAPTEGQQ
selected fasta
>CoA-transferase family protein [putative dehydratase]
GTGCGCGGAGCGAGGGAGAATTCCATGACAAGGGCAGGGCCGCTCGAGGGCATCGCCGTA
CTCATGGCCGGCCGCAGTACACCAGCCGCACTGCTGGGGAGACTGCTCGCGGACCTCGGG
GCACGTGTCGTGACGCTCTGCCGATCACCCGATCACGGGGGCCCGTTCGAGCGGTGGCTG
CACTCCGCCGCGCAGTCGGCGTCCGGTTGGGACCAGGCATCGCGTCTGCTGCAGACGGCC
GATGTGCTGGTGTGCGACGCGGAGGGCGATGAGCGGCTCGCCGCTCTGGGGCTCGGGGCG
CCGGAACTTCCGCATCGCAGCCCCGAGTTGGTCGCGGTGCGGCTGAGCGCGTTCGGGCTC
ACCGGCCCGCTGCGTGACGCCCCGGCCACCGAGCGGACCTTGCAGGCGCTGGCGGGCCTG
ACCTCGGCGACCGGCACCGAGGGGGAACCATCGGTCCTCTCCGTGGTGGGCCTGGCGTCG
CGTACGGCGGCCCTGTCCGGTCTGATCGCCGTCGTGGCCGGGCTCATCGGCCGCGAGCGC
GGAGGAGGCGGGGACTACCTGGACATCGCCGAGTTCGACAGCCTGTTCACTCTGACGGGC
ACCTTGCTTCCGAGCGTCGCGCTCGCCGGTCGTCCGCCACGTCGTACCGGCAACCGACAC
GGCATGGCGGCACCCTGGAACAGCTACACGTGCCAGGACGCCCCTGTCGTCATCTGCACG
ATGGGCGAGCCGATATGGCACAGGCTGACCGCAGTCCTGGGACGACGGGACCTCCCCGAC
GACCCTCGCTTCGCCGACACCGCCGCCCGCGTCCGCAACGCCGACGAGCTCGACGAGATC
CTCGGCAAGTGGACCGCCGGGCAGCGGGCCGTGGACGTGGTGACCGCCCTGCGGGCCGCC
GGCATCCCGTGCGCCCAGGTCGCGGCCCCCGAGGAGGTACGCGACGGAGCCGCCGCCCGG
CGTCGCGGCCTGGTCACCGACCCGTCCGGTACACCGGGCAGCCCACTGCGTTCCCTCATA
CCCGCCGTCACCGACGGTCCGATGCCCCGGCAGGGCGGCCTCTGGGAGCCGATCGCGCGC
GGCACTCCTCCGCTGCGCGGCGTCCGCCTGCTGGAGGTCGGCTCGTACACGGCCGGACCG
CACGCGGGCCGGCTGCTGGCCCAACTCGGCGCCGACGTACTGAAGGTGGAGCCGCCGCAC
GGCGAAGGGTCCCGGCGGCTCGCCCAGCAGGTGGCCGGGGTCGGCTACCTCTACTACGTC
AACAACGCGGGCAAACGCTCCTGTCGGCTCGACCTCGCCGACGCCGAGGACCGGGCCGGG
TTCGAGCGGCTGCTCGCCGGATGCGACATCGTGCTCACCAACCTCGCGGCCGACACACTC
ACCGCGCAGGGCCTCGCCCCCGATCAGATCCTGTCCCGGCACGGCGTCGTGCACTGCACG
GTCACCGGCCATGGCCTGGCCGCCGCCGACCGCAGCGTCGACACCGTGATCCAGGCCGAG
TCGGGCATCATGCGCCTCGTCGGCGGCCCGGGCGCCGGGCTGAGGACTCCAGTGTCCAGC
GCCGACGTCCTCGGTGCCTACCTGGCCGCTGCCGCCGCCGTCGTGTCCACCTACGTCCGT
CTGCGCACCGAGCACGGATGCGCCGCCGACGTCGCGCTGTTCGACAGCGCTGTCTGGCTC
ACCCAGGACCGCTGGTTCACCGCCCCACCCGCACGGGCCCCCCACCTCGTCCGGGCGGCG
GACGGCACGGTCCTCGTCGACGCGGAAGGCCCGCCACCGCGGGCCGAGGGCCCCGTCGCC
GCCGTACTGGACGCGGCGGCAGCCGTCGGCGTGCCCGCGGCCCCGCTGCACGACCTGACC
CGCGCGGTGCGGCACCCCCAAGTTCTCGCCCGGCGGATGGCCGTCGCCCGGGACTGCGCC
GGAACCACCGTGCTCATCACCGGCAACCACCTGCGTTCCCTGCTCCGCGAGGACCCGCCA
CCCACCTGCGCGCCGGTCGACCAGAACGATCCCGTCTGGCTCCAGCCCGCCCCGACCGAG
GGACAGCAATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003673 CoA-transferase family III (Family)
[66-249] 3.29999999999997e-35 PF02515 [429-567] 7.20000000000003e-26 PF02515
PF02515   CoA_transf_3
IPR023606 CoA-transferase family III domain (Domain)
[11-351] 8.10000734323267e-67 SSF89796 [361-683] 3.99998544139406e-53 SSF89796
SSF89796   CoA-Trfase_fam_III
[7-305] 2.59999999999995e-104 G3DSA:3.40.50.10540 [361-568] 2.59999999999995e-104 G3DSA:3.40.50.10540
G3DSA:3.40.50.10540   CoA-Trfase_fam_III
SignalP No significant hit
TMHMM No significant hit
Borre_00130 Borre_00130   Borre_00150 Borre_00150
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