Borre_00190 : CDS information

Location

Organism	Streptomyces parvulus
Strain	Tü4055
Entry name	Borrelidin
Contig	AJ580915
Start / Stop / Direction	39,044 / 45,514 / + [in whole cluster] 39,044 / 45,514 / + [in contig]
Location	39044..45514 [in whole cluster] 39044..45514 [in contig]
Type	CDS
Length	6,471 bp (2,156 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	borrelidin polyketide synthase, type I
Gene
Gene (GenBank)	borA5
EC number
Keyword	iterative
Note
Note (GenBank)	Module 5 (b-ketoacyl-ACP-synthase, acyltransferase, dehydratase, enoylreductase, ketoreductase, acylcarrier protein)
Reference	ACC Q70HZ8 PmId [15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004) [14651102] Evidence from engineered gene fusions for the repeated use of a module in a modular polyketide synthase. (Chem Commun (Camb). , 2003) comment [PMID: 15112998](2004) Borrelidin生合成gene clusterのクローニング。 borA5: PKS module 5(KS-ATp-DH-ER-KR-ACP) BorA5は反復性に使用され、3回のchain extensionを行う。 --- [PMID: 14651102](2003) 同じ人たちの先行する論文。 PKSの反復利用について報告している。前後のgeneとのfusionを作製して実験しており、cluster以外のPKS関連moduleの存在や、borA5のコピーが3つあって行われている見込みはなさそう。

PKS/NRPS Module

methylmalonyl-CoA

KS	60..434

AT	579..894

DH	941..1098

ER	1409..1714

KR	1724..1900

ACP	2001..2071

Sequence

>polyketide synthase [borrelidin polyketide synthase, type I]
MTSVGRRQLHVHPEDHDHHAKRTGLSMGDEQKLRTYLRRVTADLADVTERLQRAEDKNAE
PIAIVGMGCRYPGGVRSPEEFWNLLDEGVDAVAGFPEDRGWDLENLYDPDPDEPGKCYAR
EGGFLYDAGEFDAAFFGISPREALSMDPQQRLLLECSWSALERAGIDPGSLRGKDVGVYV
GAWNSNYGRGGGAESSEGHLLTGNASSVVSGRVAYVLGLEGPAVTIDTACSSSLVGLHLA
AQALRSGECGLALAGGVTVMSTPLSLVSFSRQRGLAQDGRSKAFSADADGMGMAEGVGVL
VLERLSEARRNGHEVLAVLRSSAVNQDGASNGLSAPNGPAQQRVIQSALTVGRLAPSDID
VVEAHGTGTALGDPIEAQALLATYGRGRDADRPLWLGSVKSNIGHTQAAAGVAGVIKMVL
ALRKGVLPRTLHVDEPTGEVDWDSGAVRLLTEAREWPSGEGRVRRAGVSSFGISGTNAHV
IVEEAPEEEPRPEAPSVDVVPWVLSARSAEALREQAARLASVAGGLNVVDVGWSLASTRA
AFEHRAVVVGRDSEELVSGLSSVSGVEVGVGVGAGGGVVLVFAGQGCQWVGMGRELLGSS
LVFAESMRECAAALSPFVDFSVVDVLGSAGELGRVEVVQPALWAVMVSLARVWRSWGVPV
AAVVGHSQGEIAAATVAGALSVGDAARVVALRSRLIAERLSGLGGMVSVALSRERVVSLI
AGVPGVSVAAVNGSSSTVVSGEAAGLERVLAACVSSGVRARRIDVDYASHSVQVELIREE
LLGVLDGIVPRSGEIPFVSTVTGERIDTVELGAEYWYRNLRQTVEFQASVQTLLAQGHQV
FLESSPHPVLTVGIEETVHESAAQAVVLGSLRRDEGALTRLVTSAGEAWARGVPVDWAGM
LAGGRRVELPTYPFLRERLWLEPSRSRTGNLNMAGLVEAGHEILPAAVELPGEQWVWTGE
LSLSAYPWLADHQVLGQTLVPGVAWVELALHAGHQLGFGSVEELTLQAPLVLGESDAVQV
RVVVSDLGESDRRAVSVHSRGDDQTWVTHAEGFLTAKGAQPETMAVWPPSGAEPVEADGF
YERLADAGYHYGPVFQGVSKVWRAGEEIYAEVGLLDDADVDGFGIHPALLDAALQTAYVA
QRGPAETKLPFAFGDVQLFATGARSLRVRVSPAAQQGMAWEAWDPTGLPVFSLGYLATRP
VDRGQLTVKRPESLFKVAWDETVPVVGNATAAHGVVLGDDPFALGAALRAAGWEVGAAPE
PASADTAAEVLLLPCTAPGEPDADLPTAVRAVTARVLGVLQEWLADERLAGTRLAVVTRN
ALPGDLLHSPVWGLVRSAQTENPGRITLVDLDDHPDSAAVLAEAVQSDEPRIMVREGRPT
AARLVRATAPELVPPAGADAWRLEITEPGTFDNLTLGVYPHAEKTLADNEVRVAVHAGGL
NFHDVVAALGMVEDDLTLGREAAGVVVEVGDAVPDLTPGDHVMGILSSGFGPLAVTDHRY
LARMPEGWTFAQAASVPAAFLTAYYGLCDLGGIRAGDRVLIHAAAGGVGMAAVQIARHLG
AEVFGTASPRKWGALRALGLDDAHLSSSRTLDFEQEFLDATDGRGVDLVLNSLAREFVDA
SLRLMPGGGRFVDMGKTDIRRPEQVAEDHGGVAYQAFDLVEAGPQRTGEMLAEIVRLFQA
GAFRPLPITQWDVRRAPEAFRHISQAKHIGKIVLTVPRPIDTDGTVMVTGATGTLGGFVA
RHLVTHHGIRRLLLVSRSAERTDLVRELTELGADVTWASCDLADATAVEETVRSVDERHP
LVAVVHSAGVLDDGVIDKQSPERLDTVMRPKVDAAWNLHRLLDNAPLADFVLFSSASGVL
GGAGQSNYAAANAFLDALAEHRRAQGLAGQALAWGLWSDRSTMTGQLGSTELARIARNGV
AEMSETEGLALFDAARDTAEAVLLPMHLDVARLRSRNGEVPAVFRRLIHATARRTASTAV
RSAGLEQQLASLSGPERTELLLGLVRDHAAAVLGHGTSDAVSPDRPFRDLGFDSLTAVEL
RNRFAALTGLRLPATLVFDHPSPTALAGHLAGLLGAATPSAAEPVLAAVGRLRADLRSLT
PDAEGAEDVTIQLEALLAEWREAAEKRAPEAVGDEDLSTATDDEIFALVDSELGEA

>polyketide synthase [borrelidin polyketide synthase, type I]
GTGACCTCGGTCGGCCGCCGCCAACTCCACGTACACCCCGAAGACCACGATCACCACGCG
AAAAGGACGGGCCTCTCCATGGGGGACGAGCAGAAACTCCGCACCTACCTCCGGCGCGTC
ACTGCCGACCTGGCCGACGTGACGGAGCGGTTGCAGCGAGCAGAGGACAAGAACGCCGAG
CCGATCGCGATCGTCGGCATGGGGTGCCGCTACCCCGGTGGGGTGCGGTCGCCCGAGGAG
TTCTGGAACCTGCTCGACGAAGGCGTCGACGCAGTGGCCGGCTTCCCCGAGGACCGTGGC
TGGGACCTGGAGAACCTGTACGACCCCGACCCCGACGAGCCGGGTAAGTGCTATGCCCGC
GAAGGCGGGTTCCTCTACGACGCGGGCGAGTTCGACGCCGCGTTCTTCGGGATATCGCCC
CGCGAGGCCCTGTCCATGGACCCGCAGCAGCGGCTGCTGCTGGAGTGCTCCTGGAGTGCC
CTCGAGCGGGCGGGCATCGACCCGGGCTCGCTGCGCGGCAAAGACGTCGGCGTGTACGTC
GGCGCATGGAACAGCAACTACGGCAGGGGCGGCGGGGCGGAGAGCTCCGAGGGCCACCTG
CTGACCGGCAACGCCTCCAGCGTGGTCTCGGGTCGCGTGGCGTACGTGCTGGGGCTCGAA
GGCCCCGCCGTCACCATCGACACCGCCTGTTCCTCCTCCCTGGTCGGCCTGCACCTGGCC
GCCCAGGCCCTCAGGTCCGGCGAGTGCGGTCTTGCGCTGGCCGGCGGCGTCACCGTGATG
TCCACCCCTCTGTCGCTGGTGTCCTTCTCCCGGCAGCGGGGGCTCGCACAGGACGGTCGT
TCCAAGGCGTTCTCGGCGGACGCCGATGGCATGGGCATGGCCGAAGGTGTAGGCGTACTG
GTCCTGGAGCGCCTCTCGGAGGCGCGCCGCAACGGGCACGAGGTCCTGGCCGTCCTGCGG
AGCTCGGCCGTGAACCAGGATGGTGCCTCGAACGGTCTGAGCGCCCCGAACGGGCCGGCG
CAGCAGCGTGTCATCCAGTCCGCCCTGACCGTCGGCCGTCTCGCCCCCTCCGACATCGAC
GTCGTCGAGGCCCACGGCACCGGCACGGCCCTCGGCGATCCGATCGAGGCGCAGGCGTTG
CTGGCGACGTACGGGCGGGGGCGTGATGCGGATCGTCCGTTGTGGCTGGGGTCGGTGAAG
TCGAACATCGGTCACACGCAGGCGGCCGCGGGTGTGGCCGGGGTCATCAAGATGGTGCTG
GCCCTGCGCAAGGGCGTACTGCCGCGGACGTTGCATGTGGATGAGCCAACCGGTGAGGTG
GATTGGGACTCGGGTGCGGTGCGGCTGCTGACCGAGGCGCGCGAGTGGCCGTCGGGGGAG
GGGCGTGTGCGGCGGGCGGGTGTGTCGTCGTTCGGGATCTCCGGGACGAACGCGCATGTG
ATCGTCGAGGAGGCTCCGGAGGAGGAGCCCCGGCCGGAGGCTCCTTCCGTCGACGTGGTG
CCGTGGGTTCTTTCGGCGCGGTCGGCAGAGGCGTTGCGGGAGCAGGCTGCCCGGCTGGCG
TCGGTGGCTGGTGGGTTGAACGTGGTGGATGTGGGCTGGTCGTTGGCTTCGACGCGTGCG
GCGTTCGAGCACCGGGCCGTGGTGGTGGGGCGGGACTCCGAGGAATTGGTGTCGGGGCTT
TCCTCGGTTTCGGGGGTCGAGGTCGGGGTCGGGGTCGGTGCCGGTGGTGGTGTGGTGTTG
GTGTTCGCCGGTCAGGGGTGTCAGTGGGTCGGTATGGGGCGGGAGTTGCTGGGTTCCTCG
CTGGTGTTCGCGGAGTCGATGCGGGAGTGTGCGGCGGCTCTGTCGCCGTTTGTGGACTTT
TCTGTGGTGGATGTTCTGGGTTCGGCTGGGGAGTTGGGTCGGGTCGAGGTGGTTCAGCCT
GCGTTGTGGGCGGTGATGGTGTCGCTGGCGCGGGTGTGGCGGTCGTGGGGTGTTCCGGTT
GCTGCGGTGGTGGGTCATTCGCAGGGTGAGATTGCCGCGGCGACGGTGGCGGGTGCGTTG
AGTGTGGGTGATGCGGCGCGGGTGGTGGCGTTGCGGAGCCGTTTGATCGCGGAGCGTCTG
TCGGGGCTGGGTGGGATGGTTTCGGTGGCGTTGTCGCGTGAGCGGGTGGTGTCGTTGATC
GCGGGTGTGCCGGGTGTGTCGGTGGCGGCGGTGAACGGTTCTTCGTCGACGGTGGTCTCG
GGTGAGGCCGCGGGGCTGGAGAGGGTGCTGGCCGCGTGTGTGTCGTCGGGGGTTCGGGCG
CGTCGTATCGATGTGGATTACGCCTCGCATTCGGTGCAGGTGGAGTTGATCCGTGAGGAG
TTGTTGGGGGTTCTGGACGGGATCGTCCCGCGCTCGGGTGAGATTCCGTTCGTGTCCACG
GTGACGGGTGAGCGGATCGACACTGTCGAGCTGGGGGCGGAGTACTGGTACCGCAATCTC
CGTCAGACAGTGGAATTCCAAGCATCCGTGCAGACGCTCCTCGCCCAGGGGCACCAGGTC
TTCCTGGAGTCCAGTCCGCACCCGGTTCTCACCGTCGGCATCGAGGAGACCGTTCACGAG
AGCGCCGCACAGGCCGTCGTTCTGGGAAGCCTGCGGCGGGACGAGGGTGCCCTCACCCGG
CTCGTCACCTCCGCCGGTGAGGCATGGGCGCGCGGTGTGCCCGTCGACTGGGCGGGCATG
CTCGCCGGCGGCAGGCGGGTCGAGTTGCCCACGTATCCCTTCCTCCGGGAGCGGCTGTGG
CTGGAGCCGTCGCGCTCCCGCACCGGGAACCTCAACATGGCCGGGCTGGTCGAAGCCGGA
CATGAAATCCTGCCCGCCGCAGTGGAGTTGCCCGGAGAGCAGTGGGTGTGGACCGGCGAG
CTGTCGCTCTCCGCGTACCCGTGGCTGGCCGATCACCAGGTGCTCGGGCAGACCCTGGTG
CCGGGCGTGGCGTGGGTCGAACTCGCCCTGCACGCGGGCCACCAGCTCGGTTTCGGATCC
GTCGAGGAACTCACCCTGCAGGCACCGCTCGTGCTCGGCGAGTCCGACGCCGTGCAGGTC
AGAGTCGTTGTCTCCGATCTCGGGGAGAGTGATCGCCGGGCAGTGTCGGTGCACTCGCGT
GGTGACGACCAGACGTGGGTGACCCATGCGGAGGGATTCCTCACCGCGAAAGGGGCGCAG
CCGGAGACCATGGCCGTGTGGCCGCCGTCCGGTGCGGAGCCGGTGGAGGCTGACGGGTTC
TACGAACGCCTCGCCGATGCGGGGTACCACTATGGCCCGGTCTTCCAGGGCGTGAGCAAG
GTCTGGCGAGCTGGCGAGGAGATCTACGCCGAGGTCGGGCTGCTCGACGACGCCGACGTG
GACGGCTTCGGCATCCACCCCGCCCTGCTCGACGCCGCCCTGCAGACCGCCTACGTCGCC
CAACGGGGCCCCGCAGAGACGAAGTTGCCTTTCGCGTTCGGCGATGTACAGCTGTTCGCC
ACCGGTGCCCGGTCGCTCCGCGTACGGGTCTCGCCGGCCGCTCAGCAGGGGATGGCGTGG
GAGGCCTGGGACCCCACCGGACTTCCGGTGTTCTCCCTCGGGTACCTGGCGACCCGGCCG
GTCGACCGCGGCCAGCTGACCGTGAAGCGGCCCGAGTCGCTGTTCAAGGTGGCCTGGGAC
GAGACCGTCCCCGTCGTCGGGAATGCGACCGCCGCGCATGGCGTCGTGCTGGGCGACGAC
CCGTTCGCCCTCGGTGCCGCGCTGCGCGCGGCGGGCTGGGAGGTCGGGGCCGCCCCGGAA
CCCGCGTCCGCCGACACCGCCGCCGAAGTACTGCTGCTGCCCTGCACCGCGCCCGGCGAG
CCGGACGCGGACCTGCCCACCGCGGTCAGGGCCGTGACTGCTCGGGTGCTCGGCGTCCTA
CAGGAGTGGCTCGCCGACGAACGGCTCGCCGGCACCCGACTGGCCGTCGTGACCCGCAAC
GCCCTGCCGGGTGACCTCCTGCACAGCCCCGTCTGGGGTCTCGTGCGCTCCGCCCAGACC
GAGAACCCCGGGCGCATCACCCTCGTCGACCTCGACGACCACCCCGACTCGGCGGCCGTC
CTTGCCGAGGCCGTCCAGTCCGACGAGCCGCGCATCATGGTCCGCGAGGGCCGGCCCACC
GCCGCCCGCCTGGTCCGTGCCACCGCACCCGAGCTGGTGCCGCCCGCCGGAGCCGATGCC
TGGCGCCTCGAGATCACCGAACCGGGCACGTTCGACAACCTCACGCTGGGCGTCTACCCG
CACGCCGAGAAGACCCTCGCCGACAACGAGGTCCGGGTCGCCGTCCACGCGGGCGGCCTC
AACTTCCACGACGTGGTCGCCGCACTCGGCATGGTCGAGGACGACCTGACCCTCGGCCGT
GAGGCGGCCGGCGTCGTCGTCGAGGTCGGAGACGCCGTGCCGGATCTGACCCCCGGCGAC
CACGTGATGGGCATCCTGTCCTCCGGCTTCGGGCCGCTCGCCGTCACCGATCACCGCTAC
CTGGCACGCATGCCCGAGGGCTGGACGTTCGCCCAGGCGGCTTCGGTGCCCGCCGCGTTC
CTGACGGCCTACTACGGGCTGTGCGACCTCGGCGGCATCCGCGCGGGCGACCGCGTCCTC
ATCCACGCGGCCGCCGGCGGTGTCGGCATGGCCGCCGTACAGATCGCCCGGCACCTCGGG
GCGGAGGTGTTCGGCACCGCCAGCCCGCGCAAGTGGGGCGCGCTGCGCGCCCTGGGGCTC
GACGACGCCCACCTGTCCTCCTCCCGCACCCTCGACTTCGAGCAGGAGTTCCTGGACGCC
ACCGACGGCAGGGGAGTCGACCTCGTTCTGAACTCGCTGGCCCGGGAGTTCGTCGACGCC
TCGCTGCGGCTGATGCCCGGCGGCGGCCGGTTCGTGGACATGGGCAAGACCGACATCCGG
CGGCCGGAACAGGTGGCGGAGGACCACGGCGGAGTCGCCTACCAGGCATTCGACCTCGTC
GAGGCCGGGCCGCAGCGCACGGGGGAGATGCTCGCCGAGATCGTCCGGCTCTTCCAAGCC
GGCGCGTTCCGGCCGCTGCCGATCACCCAGTGGGACGTGCGCCGGGCGCCGGAGGCCTTC
CGACACATCAGCCAGGCCAAGCACATAGGCAAGATCGTCCTCACCGTGCCCCGGCCCATC
GACACCGACGGCACCGTCATGGTCACCGGCGCCACCGGGACCCTGGGCGGCTTCGTCGCC
CGGCACCTGGTCACCCATCACGGCATACGACGACTGCTGCTGGTCAGCCGCAGCGCGGAG
CGCACCGACCTGGTGCGGGAACTCACCGAGCTGGGCGCCGACGTCACCTGGGCCTCCTGC
GACCTAGCCGACGCCACCGCCGTCGAAGAGACCGTTCGGTCCGTCGACGAACGGCATCCG
CTCGTGGCCGTCGTCCACTCTGCGGGAGTACTCGACGACGGCGTCATCGACAAGCAGAGC
CCCGAACGGCTCGACACCGTGATGCGTCCCAAGGTCGACGCCGCCTGGAATCTGCACCGA
CTCCTCGACAACGCCCCGCTGGCCGACTTCGTGCTCTTCTCCTCCGCCAGCGGCGTGCTC
GGTGGCGCCGGACAGTCCAACTACGCGGCCGCCAACGCCTTCCTCGACGCGCTCGCCGAG
CACCGCCGTGCACAGGGCCTCGCCGGACAGGCGCTCGCCTGGGGACTGTGGTCCGACCGC
AGCACGATGACGGGACAGCTCGGCTCCACCGAACTCGCCCGGATCGCCCGCAACGGCGTC
GCCGAGATGTCCGAGACGGAGGGCCTGGCCCTCTTCGACGCCGCCCGGGACACCGCCGAG
GCGGTGTTGCTGCCCATGCACCTGGACGTCGCGAGGCTCCGCAGCCGCAACGGAGAGGTA
CCCGCGGTGTTCCGCCGGCTGATCCACGCCACGGCCCGCCGCACCGCGAGCACCGCGGTC
CGCAGCGCCGGCCTCGAACAGCAGCTCGCCTCGCTGTCCGGCCCCGAACGCACGGAGCTG
CTCCTGGGACTGGTGCGCGACCATGCCGCCGCGGTGCTCGGCCACGGCACCTCCGACGCC
GTCTCGCCGGACCGGCCCTTCCGCGACCTGGGTTTCGACTCCCTGACTGCCGTGGAGCTG
CGTAACAGGTTCGCCGCCCTCACCGGCCTGCGTCTGCCGGCCACGCTCGTCTTCGACCAC
CCGAGCCCGACGGCCCTCGCCGGGCACCTCGCCGGCCTGCTGGGCGCCGCGACGCCCTCC
GCGGCCGAGCCGGTCCTGGCCGCCGTCGGACGGCTGCGCGCCGACCTCCGGTCGCTCACC
CCGGACGCCGAGGGCGCCGAGGACGTGACGATCCAGCTGGAGGCCCTCCTCGCCGAGTGG
CGGGAGGCCGCGGAGAAGCGGGCTCCGGAGGCGGTCGGTGACGAGGACCTGTCCACCGCC
ACCGACGACGAGATCTTCGCGCTCGTCGACAGCGAACTGGGTGAGGCCTGA

[5] KS	60..434

[5] AT	579..894

[5] methylmalonyl-CoA	767..771

[5] DH	941..1098

[5] ER	1409..1714

[5] KR	1724..1900

[5] ACP	2001..2071

[5] KS	178..1302

[5] AT	1735..2682

[5] methylmalonyl-CoA	2299..2313

[5] DH	2821..3294

[5] ER	4225..5142

[5] KR	5170..5700

[5] ACP	6001..6213

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR002364 Quinone oxidoreductase/zeta-crystallin, conserved site (Conserved_site) PS01162 QOR_ZETA_CRYSTAL IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) SSF47336 ACP_like PF00550 PP-binding G3DSA:1.10.1200.10 ACP_like PS50075 ACP_DOMAIN IPR011032 GroES-like (Domain) SSF50129 GroES_like IPR013154 Alcohol dehydrogenase GroES-like (Domain) PF08240 ADH_N IPR013968 Polyketide synthase, KR (Domain) PF08659 KR IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR015083 Polyketide synthase, docking (Domain) PF08990 Docking SSF101173 Polyketide_synth_docking IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR018201 Beta-ketoacyl synthase, active site (Active_site) PS00606 B_KETOACYL_SYNTHASE IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020807 Polyketide synthase, dehydratase domain (Domain) SM00826 PKS_DH IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain) SM00822 PKS_KR IPR020843 Polyketide synthase, enoylreductase (Domain) SM00829 PKS_ER
SignalP	No significant hit
TMHMM	No significant hit

Borre_00180 Borre_00200