Borre_00260 : CDS information

Location

Organism	Streptomyces parvulus
Strain	Tü4055
Entry name	Borrelidin
Contig	AJ580915
Start / Stop / Direction	57,112 / 57,858 / + [in whole cluster] 57,112 / 57,858 / + [in contig]
Location	57112..57858 [in whole cluster] 57112..57858 [in contig]
Type	CDS
Length	747 bp (248 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	putative fumarylacetoacetate hydrolase family protein
Product (GenBank)	putative 2-hydroxyhept-2,4-diene-1,7-dioate isomerase
Gene
Gene (GenBank)	borN
EC number
Keyword	trans-1,2-CPDA
Note
Note (GenBank)
Reference	ACC Q70HZ1 PmId [15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004) comment [PMID: 15112998](2004) Borrelidin生合成gene clusterのクローニング。 borN: starter unit biosynthesis closest similar to hypo borN insertional inactivation mutantはborrelidin産生できるが明らかに減少する。 borrelidin PKS starter unitであるtrans-1,2-CPDA添加でborrelidin産生レベルはやや回復する。 BorNはtyrosine代謝で2-oxohepta-3-ene-1,7-dioateの産生に関与するisomeraseに似ている。よって、borNがないときはtyrosineでの一次代謝酵素が代替しているのではないか。
Family/Domain	FD IPR002529 comment [IPR002529] Fumarylacetoacetase, C-terminal-like (Domain) PF01557 Fumarylacetoacetate (FAA) hydrolase family

Sequence

>putative fumarylacetoacetate hydrolase family protein [putative 2-hydroxyhept-2,4-diene-1,7-dioate isomerase]
MLIARAAVGEDRTYARVDTDTGLIHLLAGTPYDEIRPTGETRPLAEARLLAPVEPSKVLV
AGRNYGDVVTPDLVVFMKPSTSVVGPRSTVLLPAEAKQVRYEGELAVVIGRRCKDVPEDT
ADQAVFGYTCANDVTAWDVGEPKGHWTKAKSFDTFCPLGPWIRTDLDPADLVLRTTVNGT
LRQDGSTKEMNRNVRALVSRCSSLMTLLPGDVILTGTPAGAGVLRPGDEVVVEIDGIGSL
ANPIGVAK

>putative fumarylacetoacetate hydrolase family protein [putative 2-hydroxyhept-2,4-diene-1,7-dioate isomerase]
GTGCTGATCGCGCGCGCCGCCGTCGGAGAAGACCGAACGTACGCCCGCGTCGACACGGAC
ACAGGGCTGATCCACCTCCTGGCCGGCACTCCCTACGACGAGATCCGGCCGACCGGCGAA
ACCAGACCGCTTGCCGAGGCCCGCCTGCTCGCACCGGTCGAACCCAGCAAAGTGCTGGTC
GCAGGACGCAATTACGGCGATGTCGTCACACCGGACCTGGTGGTCTTCATGAAGCCGTCC
ACCTCTGTCGTCGGCCCCAGGAGCACCGTCCTGCTGCCGGCGGAGGCCAAGCAGGTCCGG
TACGAGGGAGAACTCGCCGTGGTGATCGGGCGCCGCTGCAAAGACGTCCCCGAAGACACC
GCGGACCAGGCCGTGTTCGGCTACACCTGCGCCAACGACGTCACCGCCTGGGACGTCGGG
GAACCGAAGGGCCACTGGACCAAGGCGAAGAGCTTCGACACATTCTGCCCGCTGGGACCA
TGGATCCGCACCGATCTCGACCCCGCTGACCTCGTCCTGCGCACAACCGTGAACGGCACG
CTGCGCCAGGACGGCTCCACCAAGGAAATGAACAGGAATGTCCGCGCCCTCGTGTCCCGT
TGCAGCTCACTGATGACGCTGCTGCCCGGAGACGTGATCCTCACCGGCACACCGGCGGGC
GCCGGCGTGCTGCGTCCGGGTGACGAGGTCGTCGTCGAGATTGACGGGATCGGTTCGCTC
GCGAATCCGATCGGCGTGGCCAAGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002529 Fumarylacetoacetase, C-terminal (Domain) PF01557 FAA_hydrolase IPR011234 Fumarylacetoacetase, C-terminal-related (Domain) SSF56529 Fumarylacetoacetase_C-rel G3DSA:3.90.850.10 Fumarylacetoacetase_C-rel IPR018833 Domain of unknown function DUF2437 (Domain) PF10370 DUF2437
SignalP	No significant hit
TMHMM	No significant hit

Borre_00250 Borre_00270