Borre_00390 Borre_00390   Borre_00410 Borre_00410

Borre_00400 : CDS information

close this sectionLocation

Organism
StrainTü4055
Entry nameBorrelidin
Contig
Start / Stop / Direction70,445 / 71,848 / + [in whole cluster]
70,445 / 71,848 / + [in contig]
Location70445..71848 [in whole cluster]
70445..71848 [in contig]
TypeCDS
Length1,404 bp (467 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative aldehyde dehydrogenase
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number1.2.1.-
Keyword
Note
Note (GenBank)
  • ORFB19
Reference
ACC
PmId
[15112998] Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055: cluster analysis and assignment of functions. (Chem Biol. , 2004)
comment
Borrelidin生合成gene clusterのクローニング。
orfB19: closest similar to Putative aldehyde dehydrogenase

borrelidin非産生であるS.coelicolorのタンパクに明らかな類似があるが、遺伝子配置は異なるもの。
Related Reference
ACC
P25526
PmId
[2254272] Molecular analysis of two genes of the Escherichia coli gab cluster: nucleotide sequence of the glutamate:succinic semialdehyde transaminase gene (gabT) and characterization of the succinic semialdehyde dehydrogenase gene (gabD). (J Bacteriol. , 1990)
[8297211] Molecular organization of the Escherichia coli gab cluster: nucleotide sequence of the structural genes gabD and gabP and expression of the GABA permease gene. (Arch Microbiol. , 1993)
[2178550] Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: isolation and characterization of a phosphinothricin-specific transaminase from Escherichia coli. (Appl Environ Microbiol. , 1990)
[12446648] The Escherichia coli gabDTPC operon: specific gamma-aminobutyrate catabolism and nonspecific induction. (J Bacteriol. , 2002)
comment
284th, 36%, 1e-70
E. coli_gabD
succinate-semialdehyde dehydrogenase [NADP+](EC 1.2.1.16)
4-aminobutyrate (GABA)の代謝経路の１つ。

<gabDTPC gene cluster>
gabD; succinate-semialdehyde dehydrogenase [NADP+](EC 1.2.1.16)
gabP; 4-aminobutyrate (GABA) transport carrier (GABA permease)
gabT; glutamate:succinic semialdehyde transaminase (EC 2.6.1.19)
gabC; repressor of the gab DTP gene cluster
ACC
O33455
PmId
[9150211] p-Cymene catabolic pathway in Pseudomonas putida F1: cloning and characterization of DNA encoding conversion of p-cymene to p-cumate. (J Bacteriol. , 1997)
comment
385th, id38%, 4e-69
Pseudomonas putida
P-cumic aldehyde dehydrogenase
cymC

E.coliで発現・タンパク抽出
NAD存在下で活性を示す。

close this sectionSequence

selected fasta
>putative aldehyde dehydrogenase [hypothetical protein]
MPTPAPDHAPAQRAAPLAVVDPATGTVFDEAPDQGPDVLDAVVDRARRAWHGWRADPDAR
TTALRSAADAVEAAGDDLARLLTREQGKPLAESHAEVARTAARLRYFAGLAPRTRRITDG
RPVRSEVRWRPLGPVAAIVPWNFPLQLASAKFAPALAAGNTMVLKPSPFTPLATRLLGSV
LATALPEDVLTVVTGREPLGARLAAHPGIRHVTFTGSVPTGRAVARAAAASLARVTLELG
GNDAAVLLDDVEVDRIADRLFWAAFRNCGQVCMAVKRVYAPARLHAQVVEALTERAKAVA
VGPGLDPRTRLGPVANAPQLARVEQITRRALADGARAAAGGHRLDGPGCFFAPTILTDVP
PDSPVVTEEQFGPVLPVLPYRSLDEAVDAANGTGFGLGGSVWGTDLDRAEAVADRLECGT
AWVNHHAELSLAQPFAGDKDSGVGVAGGPWGLYGNLRPFVVHRPRGE
selected fasta
>putative aldehyde dehydrogenase [hypothetical protein]
ATGCCGACCCCCGCACCCGACCACGCCCCCGCACAGCGGGCCGCGCCTCTCGCGGTCGTC
GATCCGGCCACCGGAACGGTCTTCGACGAGGCCCCCGACCAGGGACCGGACGTGCTGGAC
GCCGTCGTCGACCGGGCCCGCCGGGCCTGGCACGGCTGGCGCGCCGATCCCGACGCCCGT
ACCACCGCGCTGCGCTCGGCGGCCGACGCGGTCGAGGCCGCCGGGGACGACCTCGCCCGT
CTCCTCACCCGGGAACAGGGAAAGCCCCTGGCCGAATCGCATGCGGAGGTCGCCCGGACG
GCGGCCCGCCTGCGCTACTTCGCCGGCCTGGCCCCCCGGACCCGGCGCATCACCGACGGG
CGGCCGGTGCGCAGCGAGGTCCGCTGGCGCCCCCTCGGACCCGTCGCCGCGATCGTGCCG
TGGAACTTCCCCCTCCAACTCGCGTCGGCGAAGTTCGCGCCCGCGCTCGCCGCGGGCAAC
ACCATGGTCCTCAAACCCTCCCCCTTCACCCCGCTCGCCACCCGGCTGCTCGGGTCCGTC
CTCGCCACCGCCCTGCCCGAGGACGTCCTGACGGTCGTCACCGGCCGCGAGCCACTCGGC
GCCCGCCTCGCCGCACACCCCGGCATCCGCCACGTCACCTTCACCGGATCGGTGCCCACG
GGCCGGGCCGTCGCCCGAGCGGCGGCGGCCTCGCTCGCCCGGGTCACCCTGGAACTGGGC
GGCAACGACGCCGCGGTCCTGCTGGACGACGTCGAAGTGGACCGGATCGCCGACCGGCTG
TTCTGGGCCGCGTTCCGCAACTGCGGGCAGGTCTGCATGGCGGTCAAGCGCGTCTACGCA
CCGGCCCGTCTGCACGCACAGGTCGTCGAAGCCCTCACCGAGCGCGCCAAGGCCGTCGCC
GTCGGGCCCGGCCTCGACCCCCGCACCCGGCTGGGACCGGTCGCCAACGCCCCCCAGCTG
GCCCGGGTCGAGCAGATCACCCGGCGCGCCCTGGCGGACGGCGCCCGGGCGGCGGCCGGC
GGCCACCGGCTGGACGGGCCGGGCTGCTTCTTCGCCCCCACGATCCTCACCGACGTCCCG
CCCGACAGCCCGGTGGTGACCGAGGAGCAGTTCGGGCCGGTACTGCCGGTGCTGCCGTAC
CGGAGCCTGGACGAAGCCGTCGACGCGGCCAACGGCACGGGATTCGGGCTGGGGGGCTCC
GTATGGGGCACCGACCTCGACCGGGCCGAGGCGGTGGCCGACCGGCTGGAATGCGGCACG
GCCTGGGTCAACCACCACGCCGAGCTGTCCCTCGCCCAGCCCTTCGCCGGCGACAAGGAC
AGCGGGGTCGGCGTCGCGGGCGGGCCGTGGGGACTGTACGGCAACCTCCGTCCGTTCGTC
GTCCACCGACCGCGGGGGGAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR015590 Aldehyde dehydrogenase domain (Domain)
[16-452] PF00171
PF00171   Aldedh
IPR016160 Aldehyde dehydrogenase, conserved site (Conserved_site)
[237-244] PS00687
PS00687   ALDEHYDE_DEHYDR_GLU
[265-276] PS00070
PS00070   ALDEHYDE_DEHYDR_CYS
IPR016161 Aldehyde/histidinol dehydrogenase (Domain)
[13-467] SSF53720
SSF53720   Aldehyde_DH/Histidinol_DH
IPR016162 Aldehyde dehydrogenase, N-terminal (Domain)
[16-251] 7.59999999999998e-76 G3DSA:3.40.605.10
G3DSA:3.40.605.10   Aldehyde_dehydrogenase_N
IPR016163 Aldehyde dehydrogenase, C-terminal (Domain)
[252-435] 1.5e-64 G3DSA:3.40.309.10
G3DSA:3.40.309.10   Aldehyde_dehydrogenase_C
SignalP
[1-25] 0.098 Signal
Eukaryota   
TMHMM No significant hit
Borre_00390 Borre_00390   Borre_00410 Borre_00410
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