Epoth_00010 Epoth_00010   Epoth_00030 Epoth_00030

Epoth_00020 : CDS information

close this sectionLocation

Organism
StrainSo ce90
Entry nameEpothilone
Contig
Start / Stop / Direction3,171 / 1,900 / - [in whole cluster]
3,171 / 1,900 / - [in contig]
Locationcomplement(1900..3171) [in whole cluster]
complement(1900..3171) [in contig]
TypeCDS
Length1,272 bp (423 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative hydrolase
Product (GenBank)unknown
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF2; similar to hypothetical proteins from Mycobacterium tuberculosis and Streptomyces coelicolor and to DD-peptidases
Reference
ACC
PmId
[10662695] The biosynthetic gene cluster for the microtubule-stabilizing agents epothilones A and B from Sorangium cellulosum So ce90. (Chem Biol. , 2000)
comment
Table 1: deduced function
Orf2(423aa) Hypothetical protein/DD-peptidase

ORF2は、Mycobacterium tuberculosis(Z81011)とStreptomyces coelicolor(AL031107)のhypoに強い類似性が見られる。それらよりは劣るけど、Brevibacterium linensの1,4-butanediol diacrylate esterase(AB020733)や異なる細菌のcarboxypeptidases and DD-peptidasesにも類似性が見られる。epothilone生合成においてもしOrf2に役割があったとしても、直ちに明らかではない。
Related Reference
ACC
Q1I197
PmId
[16672512] Isolation and biochemical characterization of two novel metagenome-derived esterases. (Appl Environ Microbiol. , 2006)
comment
93rd(Q1I197) 37%, 7e-48
uncultured bacterium WWRS-2005_estA3
Putative beta-lactamase class C

飲料水のメタゲノム解析で得られたesterase EstA3の活性条件検討。EstA3は多量体を形成し、7-[3-octylcarboxy-(3-hydroxy-3-methyl-butyloxy)]-coumarinを含む幅広い基質に高い活性がある。

close this sectionSequence

selected fasta
>putative hydrolase [unknown]
MGALISVAAPGCALGGAEEEGQPGQDAGAGALAPAREVMAAEVAAGQMPGAVWLVARGDD
VHVDAVGVTELGGSAPMRRDTIFRIASMTKAVTATAVMMLVEEGKLDLDSPVDRWLPELA
NRKVLARIDGPIDETVPAERPITVRDLMTFTMGFGISFDASSPIQRAIDELGLVNAQPVP
MTPHGPDEWIRRLGTLPLMHQPGAQWMYNTGSLVQGVLVGRAADQGFDAFVRERILAPLG
MRDTDFHVPADKLARFAGCGYFTDEQTGEKTRMDRDGAESAYASPPAFPSGAAGLVSTVD
DYLLFARMLMNGGVHEGRRLLSAASVREMTADHLTPAQKAASSFFPGFFETHGWGYGMAV
VTAPDAVSEVPGRYGWDGGFGTSWINDPGRELIGIVMTQSAGFLFSGALERFWRSVYVAT
ESA
selected fasta
>putative hydrolase [unknown]
ATGGGAGCTTTGATCAGCGTGGCGGCGCCGGGGTGCGCGTTGGGTGGGGCCGAGGAGGAG
GGGCAGCCAGGGCAGGACGCGGGGGCCGGCGCGCTGGCGCCCGCGCGCGAGGTGATGGCG
GCGGAGGTGGCGGCGGGGCAGATGCCGGGAGCGGTCTGGCTGGTCGCGCGCGGCGACGAC
GTGCACGTGGACGCGGTGGGCGTGACCGAGCTCGGCGGCAGCGCGCCCATGCGGCGGGAC
ACGATCTTCCGGATCGCCTCGATGACGAAGGCGGTCACCGCGACGGCGGTGATGATGCTC
GTCGAGGAAGGCAAGCTCGACCTCGACTCCCCCGTCGACCGATGGTTGCCGGAGCTCGCG
AACCGGAAGGTGCTGGCGCGCATCGATGGTCCGATCGACGAGACGGTGCCCGCGGAGCGG
CCGATCACGGTGCGCGACCTGATGACGTTCACGATGGGCTTCGGAATCTCGTTCGACGCA
TCGTCGCCGATCCAGCGGGCCATCGACGAGCTCGGGCTCGTCAACGCGCAGCCGGTGCCG
ATGACGCCGCACGGGCCAGACGAGTGGATCCGGCGGCTCGGGACGCTGCCGCTCATGCAT
CAGCCGGGGGCGCAGTGGATGTACAACACGGGGAGCCTGGTGCAGGGGGTGCTCGTGGGG
CGCGCGGCCGACCAGGGCTTCGATGCGTTCGTGCGCGAGCGCATCCTCGCGCCGCTCGGG
ATGCGGGACACCGACTTTCACGTCCCGGCGGACAAGCTCGCTCGGTTCGCGGGCTGCGGT
TACTTCACCGACGAGCAGACGGGGGAGAAGACGCGCATGGATCGCGACGGGGCCGAGAGC
GCCTATGCCAGCCCGCCGGCGTTCCCTTCGGGCGCCGCCGGGCTCGTGTCGACGGTGGAC
GACTACCTCCTGTTCGCGCGCATGCTCATGAACGGCGGTGTGCACGAGGGCCGGCGGCTC
TTGAGCGCCGCCTCGGTGCGCGAGATGACGGCCGATCACCTCACGCCGGCCCAGAAGGCG
GCGTCCTCGTTTTTCCCGGGCTTCTTCGAGACGCACGGCTGGGGTTATGGGATGGCCGTG
GTCACGGCGCCGGACGCGGTCTCGGAGGTGCCCGGGCGCTATGGGTGGGACGGCGGGTTC
GGGACGTCCTGGATCAATGATCCGGGCCGCGAGCTGATCGGGATCGTGATGACACAATCC
GCCGGCTTCCTGTTCTCGGGGGCTCTCGAGCGTTTCTGGCGCAGCGTCTACGTCGCCACC
GAATCGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001466 Beta-lactamase-related (Domain)
[36-405] 2.09999999999996e-75 PF00144
PF00144   Beta-lactamase
IPR012338 Beta-lactamase/transpeptidase-like (Domain)
[36-416] 5.90000000000008e-79 G3DSA:3.40.710.10
G3DSA:3.40.710.10   G3DSA:3.40.710.10
[30-417] 6.40004254961849e-83 SSF56601
SSF56601   PBP_transp_fold
SignalP
[1-17] 0.889 Signal
Eukaryota   
TMHMM No significant hit
Epoth_00010 Epoth_00010   Epoth_00030 Epoth_00030
Page top