Monen_00270 : CDS information

Location

Organism	Streptomyces cinnamonensis
Strain	ATCC 15413
Entry name	Monensin
Contig	AF440781
Start / Stop / Direction	93,981 / 95,273 / + [in whole cluster] 93,981 / 95,273 / + [in contig]
Location	93981..95273 [in whole cluster] 93981..95273 [in contig]
Type	CDS
Length	1,293 bp (430 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450
Product (GenBank)	monensin hydroxylase
Gene
Gene (GenBank)	monD
EC number
Keyword	C-26 hydroxylate
Note
Note (GenBank)	cytochrome P450; MonD
Reference	ACC Q846W4 PmId [12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003) [8017935] Production of 26-deoxymonensins A and B by Streptomyces cinnamonensis in the presence of Metyrapone. (Appl Environ Microbiol. , 1994) comment [PMID: 12940979](2003) monensin生合成 gene clusterの解析論文。 MonD: Hydroxylase S.cinnamonensisがcytochrome P450 enzymesの強力な特異的inhibitorであるmetyrapone存在下で培養されたとき、C-26 dehydroxy-monensinsが形成された。→Ref[PMID: 8017935](1994) これに一致して、monDはpolyketide biosynthetic gene clusters由来の信頼できるcytochrome P450 enzymes(eryF hydroxylaseなど)に有意な配列類似がある。

Sequence

>cytochrome P450 [monensin hydroxylase]
MGLTVGPDNAKRGIVPITDSKPAATFPDLVDPSFWARPHAERVALFEEMRGLPRPAFIRQ
NMPGVPWTFGYHALVKYADIVEVSRRPQDFSSNGATTIIGLPPELDEYYGSMINMDNPEH
SRLRRIVSRSFGRNMIPEFEAVATRTARRIIDELIARGPGDFIRPVAAEMPIAVLSDMMG
IPAEDHDFLFDRSNTIVGPLDPDYVPDRADSERAVIEASRELGDYIAGLRAERLAAPGND
LITKLVQVQADGEQLTRQELVSFFILLVIAGMETTRNAISHALVLLTEHPEQKQLLLSDF
DTHAPNAVEEILRVSTPINWMRRVATRDCDMNGHRFRRGDRIFLFYWSGNRDESVFPDPY
RFDITRGTNAHVTFGAVGPHVCLGAHLARMEITVLYRELLAALPQIHAVGQPRRLDSSFI
EGIKHLHCAF

>cytochrome P450 [monensin hydroxylase]
GTGGGCCTGACCGTCGGTCCGGACAACGCGAAGCGAGGCATCGTGCCCATCACGGATAGC
AAGCCGGCCGCCACATTCCCCGACCTGGTCGACCCGTCGTTCTGGGCGCGGCCGCACGCG
GAACGCGTGGCGCTGTTCGAGGAGATGCGCGGGCTGCCGCGGCCGGCGTTCATCCGGCAG
AACATGCCCGGCGTGCCCTGGACGTTCGGCTACCACGCGCTGGTCAAGTACGCGGACATC
GTGGAGGTGAGCCGCCGCCCGCAGGACTTCTCCTCGAACGGCGCGACCACCATCATCGGT
CTGCCGCCCGAGCTGGACGAGTACTACGGCTCGATGATCAACATGGACAACCCGGAACAC
TCGCGGCTGCGGCGCATCGTCTCGCGTTCCTTCGGCCGCAACATGATCCCCGAGTTCGAG
GCCGTGGCGACCCGCACCGCCCGCCGCATCATCGACGAGCTCATCGCGCGGGGACCCGGC
GACTTCATCAGGCCCGTCGCCGCGGAGATGCCCATCGCCGTGCTCAGCGACATGATGGGC
ATCCCGGCGGAGGACCACGACTTCCTCTTCGACCGGTCCAACACGATCGTCGGCCCCCTC
GACCCGGACTACGTGCCGGACCGGGCGGACTCCGAACGCGCGGTGATCGAGGCGTCACGC
GAACTCGGCGACTACATCGCTGGCCTTCGTGCGGAACGGCTCGCCGCCCCCGGCAACGAC
CTCATCACCAAGCTCGTGCAAGTCCAGGCGGACGGCGAGCAGTTGACGCGGCAGGAACTC
GTCTCCTTCTTCATCCTGCTCGTCATCGCCGGGATGGAGACCACCCGCAACGCCATCTCG
CACGCGCTGGTACTGCTGACCGAGCATCCCGAGCAGAAGCAGCTGCTGCTCTCGGACTTC
GACACGCACGCGCCGAACGCGGTCGAGGAGATCCTCAGGGTCTCCACGCCCATCAACTGG
ATGCGGCGCGTCGCCACCCGCGACTGCGACATGAACGGCCACAGGTTCCGCAGGGGCGAC
CGGATCTTCCTGTTCTACTGGTCGGGCAACCGGGACGAATCCGTCTTCCCTGACCCGTAC
CGGTTCGACATCACGCGCGGGACGAACGCGCACGTCACGTTCGGCGCGGTGGGCCCGCAC
GTCTGCCTCGGGGCCCACCTCGCCCGTATGGAGATCACCGTCCTGTACCGGGAGCTGCTC
GCGGCGCTGCCCCAGATCCATGCCGTGGGGCAGCCCCGCAGGCTGGACTCCAGCTTCATC
GAAGGGATCAAGCACCTGCACTGCGCCTTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PR00385 P450 SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450
SignalP	No significant hit
TMHMM	No significant hit

Monen_00260 Monen_00280