MUP_00250 : CDS information

Location

Organism	Mycobacterium ulcerans
Strain	Agy99
Entry name	Mycolactone
Contig	BX649209
Start / Stop / Direction	20,718 / 22,457 / + [in whole cluster] 20,718 / 22,457 / + [in contig]
Location	20718..22457 [in whole cluster] 20718..22457 [in contig]
Type	CDS
Length	1,740 bp (579 aa)

Annotation

Category
Product
Product (GenBank)	putative transposase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	MUP025, -, len: 579 aa. Putative transposase, weakly similar to several putative transposases e.g. Q8R6J9 Transposase from Thermoanaerobacter tengcongensis (565 aa), fasta scores: opt: 315, E(): 5.2e-13, (22.095% identity in 525 aa overlap); O70063 Putative transposase from Mycobacterium smegmatis (503 aa), fasta scores: opt: 221, E(): 9.3e-07, (26.190% identity in 504 aa overlap); Q9Z681 Transposase from Mycoplasma mycoides subsp. mycoides SC (483 aa), fasta scores: opt: 221, E(): 9e-07, (23.288% identity in 365 aa overlap). Almost identical to MUP028 and highly similar to MUP037 but longer.

Sequence

>[putative transposase]
MAKPMAMHVARTPSAHVDKAGNARRYEAVLVRRSYRDGKKVRHQTLANLSKLPAHVIDVV
EASLKGQALVAPESVCEITRSLPHGHVAAVGAMARTLGLPALLGPRCRSRDLVLGLIISR
VLRPASKLATLAWWADTTLGEDLNATNASTGEIYEAMDWLLARQDAIEKQLAAKHLAASV
NPSRMALFDLSSSWMTGQCCDLAARGYSRDGKKGLPQIEYGLLTDPAGRPVAIRVFAGNT
ADPAAFTDIVEVVRERFGLDRLVLVGDRGMITSARIAALRELNNDPDTATGFGWITALRA
PAIAKLAGDDGPLQLSLFDTQDLATITHPDYPSERLIACRNPLLATQRARKRAELLTVTE
AALAPIIAAVACGRLAGAGRIGVKVGKVLAKFKMAKHFHLDITDTTLTVTRDQTKIDAEA
ALDGIYVLRTSVTANELDPAAVVVSYKNLANVERDFRSIKTDDLDLRPIHHRLDDRVKAH
ILIAMLACYLVWHLRKAWAPMTYTDENPPARENPVTPAQRSAAAKTKAARHQDANGATLR
SFSGLLEHLATLTRNDVNFTHTTNPIPMLATPPRPTTRL

>[putative transposase]
ATGGCCAAGCCGATGGCGATGCACGTAGCCCGTACGCCGAGTGCGCATGTGGATAAGGCG
GGCAACGCGCGCCGCTACGAGGCGGTGCTGGTGCGGCGAAGCTATCGCGACGGCAAAAAG
GTTCGACATCAGACCCTGGCGAATCTGTCCAAGCTGCCTGCCCACGTTATCGACGTGGTC
GAGGCCAGCCTCAAGGGCCAGGCGCTGGTAGCGCCCGAGTCGGTCTGTGAGATCACACGT
TCACTGCCCCATGGGCATGTGGCCGCGGTGGGGGCCATGGCACGCACGCTGGGGCTGCCC
GCACTGTTGGGGCCGCGGTGTCGGTCCCGCGATCTGGTGTTGGGGTTGATCATCTCGCGG
GTGCTCCGACCTGCCTCCAAGCTGGCCACGCTGGCCTGGTGGGCCGACACCACACTCGGC
GAAGATTTGAACGCGACCAACGCATCGACCGGCGAGATCTATGAGGCGATGGACTGGCTG
CTCGCCCGTCAAGACGCGATCGAAAAACAGTTGGCTGCCAAACACCTGGCCGCCTCGGTC
AATCCGTCGCGGATGGCGCTGTTTGACTTGTCCTCATCGTGGATGACCGGCCAATGCTGC
GATCTGGCTGCACGCGGGTATTCCCGTGACGGTAAGAAGGGCCTACCCCAAATCGAGTAC
GGGCTGCTCACCGACCCCGCTGGGCGCCCAGTGGCGATACGGGTGTTCGCCGGTAACACC
GCTGACCCGGCCGCGTTCACCGACATCGTCGAGGTGGTGCGTGAGCGCTTCGGGCTGGAC
CGGCTGGTTCTTGTCGGTGATCGCGGCATGATCACCTCCGCGCGTATCGCCGCGCTGCGC
GAACTCAACAACGACCCCGACACCGCAACCGGTTTCGGCTGGATCACCGCGCTGCGTGCA
CCGGCGATCGCCAAGCTCGCCGGTGATGACGGGCCGCTGCAACTGAGCCTGTTCGATACC
CAAGACCTGGCCACCATCACCCACCCCGACTATCCCAGCGAACGACTCATCGCGTGTCGT
AACCCACTGTTGGCGACCCAACGCGCCCGCAAACGTGCCGAATTGCTGACCGTCACCGAG
GCTGCGCTGGCGCCGATCATCGCCGCAGTAGCTTGTGGCCGGCTGGCCGGCGCCGGGCGG
ATCGGGGTGAAAGTTGGCAAAGTACTGGCAAAGTTCAAGATGGCCAAACACTTCCATCTC
GACATCACCGACACCACACTGACCGTGACGCGTGACCAAACCAAGATCGATGCCGAAGCC
GCCCTGGATGGGATCTACGTGCTGCGCACCAGCGTCACCGCCAACGAGCTTGACCCCGCC
GCAGTCGTGGTCAGCTATAAAAACCTCGCCAACGTCGAACGCGACTTCCGCAGCATCAAA
ACCGACGACCTGGACCTACGCCCCATTCATCACCGACTCGACGACCGGGTCAAAGCCCAC
ATCCTGATCGCCATGCTCGCCTGCTACCTGGTGTGGCACCTACGCAAAGCCTGGGCACCC
ATGACCTATACCGACGAAAACCCACCAGCCCGGGAAAACCCTGTCACACCCGCCCAACGC
TCAGCTGCCGCCAAAACCAAAGCCGCACGCCACCAAGACGCCAACGGAGCCACGCTACGC
AGCTTCAGCGGCCTGCTCGAGCACCTGGCAACCCTGACCCGCAACGACGTCAACTTCACC
CACACCACCAACCCGATCCCGATGCTCGCCACCCCACCCCGACCAACAACGCGCCTTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002559 Transposase, IS4-like (Domain) PF01609 DDE_Tnp_1 IPR012337 Ribonuclease H-like domain (Domain) SSF53098 RNaseH_fold
SignalP	No significant hit
TMHMM	No significant hit

MUP_00240 MUP_00260