MUP_00530 : CDS information

Location

Organism	Mycobacterium ulcerans
Strain	Agy99
Entry name	Mycolactone
Contig	BX649209
Start / Stop / Direction	148,859 / 147,546 / - [in whole cluster] 148,859 / 147,546 / - [in contig]
Location	complement(147546..148859) [in whole cluster] complement(147546..148859) [in contig]
Type	CDS
Length	1,314 bp (437 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	probable cytochrome p450 150 cyp150
Gene
Gene (GenBank)	cyp150
EC number
Keyword	side chain C-12 hydroxylate
Note
Note (GenBank)	MUP053c, cyp150, len: 437 aa. Probable cyp150, cytochrome p450 (EC 1.14.-.-), similar to O08464\|C140_MYCTU\|Rv1880c Probable cytochrome P450 140 from M. tuberculosis (438 aa), fasta scores: opt: 1873, E(): 6.7e-117, (62.529% identity in 435 aa overlap). And weakly similar to numerous others e.g. Q89G11\|BAC51802 Bll6537 protein from Bradyrhizobium japonicum (433 aa), fasta scores: opt: 755, E(): 6.1e-41 (34.925% identity in 398 aa overlap); and Q9X8Q3 Putative cytochrome P450 from Streptomyces coelicolor (411 aa), fasta scores: E(): 2.9e-36, (34.063% identity in 411 aa overlap). Contains a Pfam match to entry PF00067, Cytochrome P450. Belongs to the cytochrome P450 family.
Reference	ACC Q6MZ58 PmId [14736915] Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulcerans. (Proc Natl Acad Sci U S A. , 2004) comment mycolactone合成に必要十分なclusterを有する174kb plasmid pMUM001の報告。 P450 monooxygenase (mup053)はたぶん側鎖のC12でのhydroxylationを担う。
Related Reference	ACC Q00441 PmId [2011746] An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea. (Science. , 1991) [1732208] Characterization of Saccharopolyspora erythraea cytochrome P-450 genes and enzymes, including 6-deoxyerythronolide B hydroxylase. (J Bacteriol. , 1992) [7749919] Structure of cytochrome P450eryF involved in erythromycin biosynthesis. (Nat Struct Biol. , 1995) comment 57th, id39%, 1e-48 Saccharopolyspora erythraea_eryF(CYP107A1) 6-deoxyerythronolide B hydroxylase --- [PMID: 2011746](1991) eryF(CYP107)破壊で6-deoxyerythromycin A産生。よってeryFは6-deoxyerythronolide B (DEB) hydroxylaseをコードし、DEB → erythronolide B (EB)への変換を担う。通常はEBで働く酵素が代替基質DEBを加工できたのでC-6 OH基を欠いた生物学的に活性のあるerythromycin派生体が形成された。 --- [PMID: 1732208](1992) catalytically active cytochrome P-450 fractionより、majorなものとminorな種類の P-450 を精製し、各々の遺伝子orf405(major) とeryF(minor)の遺伝子をクローニングしている。また、各々の遺伝子をE. coliで発現させて活性を調べ、EryFは6-deoxyerythronolide B hydroxylase activityを持つのに対して、Orf405は6-deoxyerythronolide B hydroxylase activityを持たないこと、EryF, Orf405共に弱いながらも7-ethoxycoumarinのO-dealkylation活性を持つことが示されている。 --- [PMID: 7749919](1995) Saccharopolyspora erythraeaのCytochrome P450eryFは6-deoxyerythronolide Bの6S-hydroxylationを触媒する(6-deoxyerythronolide Bを抗生物質erythromycinに変換するための第一ステップ)。Cytochrome P450eryFの結晶構造解析。

Sequence

>putative cytochrome P450 [probable cytochrome p450 150 cyp150]
MRQRLNWIAAHGLLRGTARLAARLGDVQSRLVADPMVMANPAPFCDELRAIGPVVSSYGT
HLVVSHAIAHELLRSEDFEVVSLGSNLPAPMRWLERRTRDDTPHLLLPPSLLAVEPPNHT
RYRKAVSSVFTPKAVAGLRDHVEETASALLDQLTDQASAVDIIARYCSQLPVAVICDILG
VPSRDRNRVLKFGQLAGPCLDFGLTWRQHQQVRQGLQGLHFWITEHLEELRSNPGDDLMS
QMIHASENGSSETHLHATEVRMIGLVLGASFATTMDLLGNGIQVLLDAPELRDALSQRPQ
LWPNAVEEILRLEPPVQLAGRMARKDTEVAGTAIKRGQLVAIYLGAVNRDPSVFADPHRF
DITRANANRHLAFSGGRHFCLGAALARVEGEVGLRMLFERFPDVRAAGPGNRRDTRTLRG
WSQLPVQLGAARSMAIR

>putative cytochrome P450 [probable cytochrome p450 150 cyp150]
GTGAGGCAGAGATTGAACTGGATTGCGGCGCACGGGTTGCTCCGCGGCACCGCGCGGTTA
GCGGCCCGGCTGGGCGACGTGCAGTCGCGGCTGGTGGCAGATCCCATGGTTATGGCAAAC
CCGGCGCCATTTTGCGATGAATTGAGGGCAATCGGCCCTGTGGTGTCGAGCTACGGCACC
CACCTCGTCGTTAGTCATGCCATCGCCCATGAACTGCTTCGGTCCGAAGACTTCGAAGTG
GTCTCGCTCGGATCGAACTTGCCGGCACCAATGCGCTGGCTAGAGCGCCGCACTCGGGAC
GATACGCCCCATCTGCTGCTGCCGCCGTCGTTGCTGGCCGTTGAGCCGCCGAATCACACG
CGCTATCGCAAGGCAGTGTCCTCGGTGTTCACGCCGAAAGCAGTAGCCGGATTACGCGAT
CATGTCGAAGAGACTGCGTCGGCGCTGTTGGATCAGCTCACCGACCAGGCTAGTGCCGTC
GACATCATAGCCCGCTACTGCTCCCAGCTGCCGGTCGCGGTCATTTGTGACATCTTAGGC
GTGCCCAGTCGAGACCGAAACCGTGTTCTCAAGTTCGGTCAGCTGGCGGGGCCCTGCTTG
GATTTTGGGCTCACATGGCGTCAGCACCAGCAGGTGCGGCAAGGGCTCCAAGGACTCCAC
TTCTGGATCACCGAGCACCTCGAGGAATTGCGGTCTAACCCCGGTGACGATCTGATGAGT
CAAATGATCCACGCAAGTGAAAATGGCTCCTCGGAAACACACCTCCACGCAACCGAAGTG
CGGATGATCGGGCTGGTGTTGGGCGCCAGTTTCGCAACAACGATGGACCTGTTAGGCAAC
GGGATTCAGGTGTTGTTGGACGCGCCCGAACTGCGGGACGCGTTGAGTCAGCGCCCGCAA
CTTTGGCCCAACGCGGTAGAAGAGATCCTGCGGTTGGAGCCACCGGTTCAGCTCGCCGGA
CGAATGGCTCGCAAGGACACCGAGGTGGCGGGTACCGCAATCAAGCGGGGCCAGCTGGTG
GCGATCTATCTGGGGGCGGTCAACCGTGATCCGTCCGTGTTCGCCGATCCGCACCGCTTT
GACATCACACGAGCCAACGCCAACCGGCATCTCGCATTCTCCGGTGGCCGCCACTTCTGC
CTCGGTGCCGCCCTTGCCCGCGTCGAAGGCGAAGTCGGATTGAGAATGCTCTTCGAGCGC
TTCCCTGACGTGCGCGCCGCAGGCCCCGGAAATAGACGTGATACTCGAACTCTGCGGGGT
TGGTCGCAGCTGCCGGTCCAGTTGGGCGCAGCACGATCGATGGCTATCCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

MUP_00520 MUP_00540