Myxo_00010 : CDS information

Location

Organism	Stigmatella aurantiaca
Strain	DW4/3-1
Entry name	Myxothiazol
Contig	AF188287
Start / Stop / Direction	1 / 834 / + [in whole cluster] 1 / 834 / + [in contig]
Location	1..834 [in whole cluster] 1..834 [in contig]
Type	CDS
Length	834 bp (277 aa)

Annotation

Category	1.4 Other
Product	phosphopantetheinyl transferase
Product (GenBank)	MtaA
Gene
Gene (GenBank)	mtaA
EC number	2.7.8.-
Keyword
Note
Note (GenBank)	Ppant transferase
Reference	ACC Q9RFL1 PmId [10601310] New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1. (J Biol Chem. , 1999) [11134965] The mtaA gene of the myxothiazol biosynthetic gene cluster from Stigmatella aurantiaca DW4/3-1 encodes a phosphopantetheinyl transferase that activates polyketide synthases and polypeptide synthetases. (J Biochem. , 2001) comment [PMID: 10601310](1999) Myxothiazol生合成gene clusterの同定論文。 MtaA: Ppant transferase Ppant=4'-phosphopantetheinyl 4'-phosphopantetheinyl transferase MtaAによってmyxothiazol合成は活性化される。 mtaAへのkanamycin resistance geneの挿入はmyxothiazol合成を阻害する。 MtaB-MtaGのACP, PCP domainは4'-phosphopantetheine cofactorのためのputative binding siteのProsite consensus signatureを含んでいる。 -- [PMID:11134965](2001) MtaAをACP, PCPと異種性発現して、4'-phosphopantetheinyl transferases機能があることを確認。 MtaAは他種のACP, PCPも同様に加工でき、基質特異性がゆるい。
Related Reference	ACC P39135 PmId [9484229] Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases. (Biochemistry. , 1998) comment 171st(P39135) 34%, 3e-17 B.subtilis_sfp 4'-phosphopantetheinyl transferase sfp(EC 2.7.8.-) phosphopantetheinyl transferases (PPTases)活性を測定

Sequence

>phosphopantetheinyl transferase [MtaA]
MPTSSPALPLLKLPPDEVHVWIVEPERITEPGLLESYRALLDPGERDKQQRFYFERHRLQ
YLVSHALVRLTLSRYAPVAPEAWSFSANQYGRPEIRGEEKPWLRFNLSHTDGMALCAVAR
DVDVGADVEDTERRGETVEIADSFFAPAEVASLRALPVSGQRERFFDYWTLKEAYIKARG
MGLSLPLDQFAFEVSQGLSTRISFDPRLVDEPSQWQFVRFRPSQRHAAALAVRRPSEAPL
TVRFQRTVPLQDDAPAEYLSRERIQPLRLRMPGVGGG

>phosphopantetheinyl transferase [MtaA]
ATGCCGACGTCCTCCCCTGCGCTGCCCTTGTTGAAGTTGCCACCAGACGAAGTCCATGTC
TGGATCGTCGAGCCCGAGCGCATCACCGAGCCAGGGCTGCTGGAGTCCTACCGCGCCCTG
CTGGACCCAGGGGAGCGCGACAAACAGCAGCGGTTCTACTTCGAGAGGCACCGGCTGCAG
TACCTCGTCTCTCATGCGCTCGTGCGGCTCACCTTGTCGCGCTATGCGCCCGTGGCGCCC
GAGGCCTGGTCCTTCTCCGCCAACCAGTACGGCCGGCCGGAGATCCGGGGTGAGGAGAAA
CCGTGGTTGCGTTTCAACCTCTCCCACACGGATGGGATGGCCCTCTGCGCGGTGGCCCGC
GATGTGGATGTGGGAGCCGATGTGGAGGACACCGAGCGGAGAGGGGAAACGGTGGAGATC
GCCGACAGCTTTTTCGCCCCCGCCGAGGTGGCCTCGCTTCGGGCACTCCCGGTGAGTGGC
CAGCGCGAGCGCTTCTTTGATTATTGGACCTTGAAGGAGGCCTACATCAAGGCGCGTGGA
ATGGGGCTTTCCCTGCCCTTGGACCAGTTCGCCTTCGAGGTTTCGCAGGGGCTTTCCACC
CGGATTTCCTTTGATCCGCGTCTGGTGGACGAGCCCTCTCAGTGGCAGTTCGTACGGTTC
CGGCCCTCCCAGCGGCACGCCGCCGCGCTGGCTGTGCGCCGCCCCTCGGAGGCCCCCCTC
ACGGTGCGGTTTCAGCGCACCGTTCCGCTCCAGGATGATGCACCTGCGGAGTATCTCTCA
CGAGAGCGGATCCAGCCGCTGCGCCTCCGGATGCCCGGGGTGGGAGGAGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR008278 4'-phosphopantetheinyl transferase (Domain) PF01648 ACPS SSF56214 4'-phosphopantetheinyl transferase G3DSA:3.90.470.20 no description
SignalP	No significant hit
TMHMM	No significant hit

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