Nanch_00170 Nanch_00170   Nanch_00190 Nanch_00190

Nanch_00180 : CDS information

close this sectionLocation

Organism
StrainNS3226
Entry nameNanchangmycin
Contig
Start / Stop / Direction71,140 / 70,145 / - [in whole cluster]
71,140 / 70,145 / - [in contig]
Locationcomplement(70145..71140) [in whole cluster]
complement(70145..71140) [in contig]
TypeCDS
Length996 bp (331 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative dTDP-glucose 4,6-dehydratase
Product (GenBank)NanG2
Gene
Gene (GenBank)nanG2
EC number4.2.1.46
Keyword
  • 4-O-methyl-L-rhodinose
Note
Note (GenBank)
  • dTDP-D-glucose-4,6-dehydratase
Reference
ACC
PmId
[12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003)
comment
Nanchangmycin生合成のためのcomplete gene clusterの報告。
（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）

配列解析からの機能予測。
NanG2: dTDP-D-glucose-4,6-dehydratase

6-deoxysugar生合成の早期段階に関与。
Related Reference
ACC
Q194R3
NITE
Mith_00070
PmId
[9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997)
comment
12th(Q194R3) 71%, 1e-127
Streptomyces argillaceus_mtmE
dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

E. coli発現タンパクで活性みている。
mtmD: glucose-1-phosphate:dTTP thymidylyl transferase
mtmE: dTDP-D-glucose 4,6-dehydratase

close this sectionSequence

selected fasta
>putative dTDP-glucose 4,6-dehydratase [NanG2]
MKLLVTGAAGFIGSTYARRLLARGGAEWGPDVSHVTVLDKLTYAGTLSNLDTADPRLTFV
HGDICDADLVDTLMARANQVVHFAAESHVDRSITGADPFVRTNVEGTHTLLQAALRHGVE
RFVHVSTDEVYGSVETGFSPETAVLDPNSPYAASKAASDLIALAYHRTHGLDVRVTRCSN
NYGPHQFPEKIIPLFITNLLDGEDVPLYGDGLNVRDWLHVEDHCRGVELVRTKGSPGEIY
NIGGGTALTNRELTGRLLDACGAGWSRVRYVEDRKGHDRRYAVQDDKARDELGYRPRHDF
AAGLAETVAWYRDNRPWWEPLKRSALTGGSR
selected fasta
>putative dTDP-glucose 4,6-dehydratase [NanG2]
ATGAAGCTCCTGGTCACAGGTGCCGCGGGGTTCATCGGGTCCACCTATGCACGCAGGCTC
CTCGCACGGGGCGGCGCGGAGTGGGGCCCCGACGTCTCACATGTCACCGTGCTGGACAAG
CTCACCTACGCGGGGACACTGAGCAACCTCGACACCGCCGACCCCCGGCTGACGTTCGTG
CACGGCGACATCTGCGACGCCGACCTGGTCGACACGCTGATGGCGCGGGCCAACCAAGTG
GTGCACTTCGCCGCCGAATCCCACGTCGACCGCTCCATCACCGGCGCCGACCCGTTCGTG
CGCACCAATGTCGAGGGCACCCACACGCTGCTGCAGGCGGCCCTGCGCCACGGGGTGGAG
CGTTTCGTCCATGTCTCCACCGACGAGGTCTACGGCTCGGTCGAGACCGGATTCAGCCCC
GAGACCGCCGTACTGGACCCCAACTCGCCGTACGCCGCCTCCAAGGCCGCCTCCGACCTG
ATCGCCCTCGCCTACCACCGCACCCATGGGCTGGACGTACGCGTCACCCGGTGCAGCAAC
AACTACGGGCCCCACCAGTTCCCGGAAAAGATCATCCCGCTGTTCATCACCAACCTGCTC
GACGGCGAGGACGTGCCGCTGTACGGCGACGGGCTGAACGTGCGCGACTGGCTGCACGTG
GAGGACCACTGCCGCGGCGTGGAACTGGTGCGCACCAAGGGATCGCCCGGCGAGATCTAC
AACATCGGCGGCGGCACCGCGTTGACCAACCGGGAGCTGACCGGTCGGCTGTTGGACGCC
TGCGGCGCCGGCTGGAGCCGCGTACGGTACGTCGAGGACCGCAAGGGGCACGACCGGCGC
TACGCGGTCCAGGACGACAAGGCCAGGGACGAACTCGGCTACCGCCCGCGCCACGACTTC
GCGGCCGGGCTGGCCGAGACGGTCGCCTGGTACCGGGACAACCGCCCCTGGTGGGAGCCG
CTCAAACGCTCCGCGCTCACCGGTGGCTCCCGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[4-243] 5.09999999999993e-67 PF01370
PF01370   Epimerase
IPR005888 dTDP-glucose 4,6-dehydratase (Family)
[2-321] 2.70000000000002e-122 TIGR01181
TIGR01181   DTDP_gluc_dehyt
IPR016040 NAD(P)-binding domain (Domain)
[1-199] 1.9e-64 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-17] 0.369 Signal
Bacteria, Gram-negative   
[1-17] 0.949 Signal
Eukaryota   
[1-22] 0.328 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Nanch_00170 Nanch_00170   Nanch_00190 Nanch_00190
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