Nanch_00280 : CDS information

Location

Organism	Streptomyces nanchangensis
Strain	NS3226
Entry name	Nanchangmycin
Contig	AF521085
Start / Stop / Direction	101,467 / 100,196 / - [in whole cluster] 101,467 / 100,196 / - [in contig]
Location	complement(100196..101467) [in whole cluster] complement(100196..101467) [in contig]
Type	CDS
Length	1,272 bp (423 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	NanP
Gene
Gene (GenBank)	nanP
EC number
Keyword	C-30 hydroxylate
Note
Note (GenBank)	putative cytochrome P450
Reference	ACC Q7WTD5 PmId [12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003) comment nanchangmycin生合成のためのcomplete gene clusterの報告。（complete clusterと言っているが、同定されたclusterがNanchangmycin生合成に十分かは確認されていない。）配列解析からの機能予測 NanP: Cytochrome P450 おそらくC-30におけるmethyl基の酸化に関連している。
Related Reference	ACC Q846W4 NITE Monen_00270 PmId [8017935] Production of 26-deoxymonensins A and B by Streptomyces cinnamonensis in the presence of Metyrapone. (Appl Environ Microbiol. , 1994) comment 2nd(Q846W4) 68%, 1e-163 Streptomyces cinnamonensis_monD Monensin hydroxylase [Monen_00270]cytochrome P450(C-26 hydroxylate) S. cinnamonensisをcytochrome P450 enzymesの強力な特異的inhibitorであるmetyrapone存在下で培養した時、C-26 dehydroxy-monensinsが形成されたことが示されている。

Sequence

>putative cytochrome P450 [NanP]
MNRGVVSPTEATPASSAKATRPPDFMDPSFWLRPRDERAEVFEKLRALPGPEFVPPRLPW
GPLASGYYALSKHADICEVSRRPQDFSSEGATAILPPEMDEFYGSMINMDNPEHSRLRRI
VARSFGRGMAPKFDAMSRRVARRIVDELIERGPGDFIRPAAEMPIAVLSTMMGIPGEDYE
FLFERTNTIMGGADPELAADPEKMAAAVLGALRDLGDYIGRLREDRLARPGPDVITKLVQ
VQEDGEQLTNQELVSFFILLINAGMETTRNVIAQALVLLTEHPDQRQLLLSDFELHAKGA
VEEILRVGTPINWMRRTATGDCEMNGHRFRKGDEIFLFYWSANHDEKVFEDAYRFDITRD
PNPHLSFGAVGPHFCLGAHLARIEIIAMLRELLASLPDIRVEGEPVRLASSFIEGFKELS
CTF

>putative cytochrome P450 [NanP]
TTGAATCGAGGCGTTGTGTCTCCTACGGAAGCCACCCCCGCATCGTCCGCCAAAGCCACC
AGGCCTCCCGACTTCATGGATCCGTCCTTCTGGCTGCGGCCGCGTGATGAGCGGGCCGAG
GTGTTCGAGAAGCTGCGCGCGCTGCCGGGCCCGGAATTCGTTCCGCCGCGGCTGCCCTGG
GGGCCGTTGGCATCCGGTTATTACGCGCTGTCGAAACACGCCGACATCTGCGAGGTGAGC
CGCCGCCCTCAGGACTTCTCCTCGGAGGGCGCCACGGCGATCCTGCCGCCGGAGATGGAC
GAGTTCTATGGCTCCATGATCAACATGGACAATCCGGAGCACTCCAGATTACGGCGCATC
GTCGCGCGGTCGTTCGGCCGAGGAATGGCGCCCAAGTTCGACGCCATGTCCCGGCGGGTG
GCCCGGCGGATCGTGGACGAGCTCATCGAGCGCGGCCCCGGCGACTTCATCCGCCCGGCG
GCGGAGATGCCGATCGCCGTCCTCAGCACCATGATGGGAATTCCGGGGGAAGACTACGAA
TTCCTCTTCGAGCGGACGAACACGATCATGGGAGGGGCTGATCCCGAACTCGCGGCCGAC
CCGGAGAAGATGGCGGCGGCGGTGCTGGGAGCCCTGCGAGACCTCGGAGACTACATCGGA
CGGCTCCGCGAGGACCGGCTCGCCCGTCCCGGCCCCGATGTGATCACCAAGCTGGTCCAG
GTGCAGGAAGACGGAGAGCAACTGACCAACCAGGAACTGGTCTCGTTCTTCATCCTCCTC
ATCAACGCGGGCATGGAGACGACCCGCAACGTCATCGCCCAGGCCCTGGTTCTGCTGACC
GAACATCCTGATCAGCGGCAACTCCTGCTGTCGGACTTCGAACTCCACGCGAAGGGCGCC
GTCGAGGAGATCCTCCGGGTGGGCACGCCCATCAACTGGATGCGCCGGACCGCGACCGGC
GACTGCGAGATGAACGGCCATCGGTTCCGCAAGGGCGACGAGATCTTCCTCTTCTACTGG
TCCGCCAACCACGACGAGAAGGTCTTCGAGGATGCGTACCGGTTCGACATCACACGGGAC
CCCAACCCGCATCTGTCGTTCGGCGCGGTGGGGCCGCACTTCTGCCTCGGTGCTCATCTC
GCCCGGATCGAGATCATCGCCATGCTCCGGGAACTCCTCGCCTCACTGCCGGACATCCGC
GTGGAGGGCGAGCCGGTCCGGCTGGCATCGAGCTTCATTGAGGGCTTCAAAGAGTTGAGC
TGCACCTTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450
SignalP	No significant hit
TMHMM	No significant hit

Nanch_00270 Nanch_00290