Nanch_00320 Nanch_00320   last last

Nanch_00330 : CDS information

close this sectionLocation

Organism
StrainNS3226
Entry nameNanchangmycin
Contig
Start / Stop / Direction107,876 / 106,815 / - [in whole cluster]
107,876 / 106,815 / - [in contig]
Locationcomplement(106815..107876) [in whole cluster]
complement(106815..107876) [in contig]
TypeCDS
Length1,062 bp (353 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative glycosidase
Product (GenBank)NanG8
Gene
Gene (GenBank)nanG8
EC number3.2.1.-
Keyword
Note
Note (GenBank)
  • putative secreted endoglucanase
Reference
ACC
PmId
[12770825] A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. (Chem Biol. , 2003)
comment
nanchangmycin生合成のためのcomplete gene clusterの報告。
（complete clusterと言っているが、同定されたclusterのみでNanchangmycinが生合成できるかは確認されていない。）

nanG8はCellulomonas fimiのputative secreted endoepoxiglucanase CenAをコードするglucosyl hydrolaseに似ている。おそらく一次代謝に関係し、nanchangmycin gene clusterには含まれない。
Related Reference
ACC
P07984
PmId
[3023193] Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi. (Gene. , 1986)
comment
17th(P07984) 41%, 5e-52
Cellulomonas fimi_cenA
Endoglucanase A(EC 3.2.1.4)
synonym: Cellulase A
synonym: Endo-1,4-beta-glucanase A

C末にある領域がendoglucanase活性に重大であることが確認されている。

close this sectionSequence

selected fasta
>putative glycosidase [NanG8]
MAEKGLGRATARHWAPLAGCAAALLALSGCLGTSGGSDRRSGLWVDPDNPAARQAREQRR
AGRYAEAELMRRIADEPIAEWLGPHPRERVRFITESAAREHRTPVLVAYHIPYRDCGRYS
RGGASSDAEYLDWIREIVSGIGERRAIVVLEPDAIAQVVDGCIPKPLRAPRLALLRDAVA
MLSAMPATRIYLDAGNPGWIKDTERLVEPLMKSGIDQADGFALNVSNFQPTAVTRAYGRR
LSLQLGRVHFIIDTSRNGNGPLTSVRGEGDDGKSGDMGAVRSASDGQESWCNPPGRALGD
PPSTRTGDPAIDAYLWIKRPGESDGTCNGGPPAGRWWTEYALDLARNARLRAR
selected fasta
>putative glycosidase [NanG8]
ATGGCCGAGAAAGGGCTCGGGAGAGCCACCGCGCGACATTGGGCGCCGCTTGCGGGATGT
GCCGCCGCGCTCCTCGCGCTGAGCGGATGTCTCGGCACGTCAGGCGGGTCGGACCGGCGG
AGCGGGCTATGGGTTGACCCGGACAACCCGGCCGCCCGGCAGGCCCGTGAGCAGCGGCGC
GCGGGCCGTTACGCGGAGGCGGAGCTCATGCGGCGGATCGCCGACGAGCCGATCGCCGAA
TGGCTCGGCCCGCATCCGCGCGAACGGGTCCGGTTTATCACCGAATCCGCAGCGCGGGAG
CACCGTACGCCGGTGCTGGTCGCCTATCACATTCCGTACCGGGACTGCGGAAGATACTCC
CGGGGCGGGGCGAGCAGCGACGCCGAATATCTGGACTGGATCCGTGAGATCGTCTCCGGA
ATCGGCGAGCGAAGGGCGATTGTCGTATTGGAGCCCGACGCGATCGCTCAGGTGGTCGAC
GGCTGCATTCCCAAGCCGCTGCGTGCGCCGCGGCTGGCCCTGCTGCGCGACGCGGTCGCC
ATGCTCTCCGCGATGCCCGCCACCCGGATCTATCTGGACGCCGGGAACCCGGGGTGGATC
AAGGACACCGAGCGGCTGGTGGAGCCGCTGATGAAAAGCGGTATCGACCAGGCGGACGGC
TTCGCCCTGAACGTCTCGAACTTCCAGCCGACAGCGGTGACCCGCGCCTACGGCCGTCGG
CTGTCCCTCCAGCTCGGCAGGGTCCACTTCATCATCGACACCAGCCGCAACGGCAACGGC
CCGCTCACCTCCGTCAGGGGCGAGGGCGACGACGGCAAGAGCGGTGACATGGGCGCCGTG
CGCTCCGCCAGCGACGGCCAGGAGAGCTGGTGCAACCCGCCGGGGCGGGCTCTGGGGGAT
CCCCCCAGCACCCGTACGGGCGACCCCGCCATCGACGCGTATCTGTGGATCAAGCGTCCG
GGAGAGTCCGACGGGACCTGCAACGGCGGTCCGCCCGCAGGTCGTTGGTGGACGGAGTAC
GCGCTCGACCTGGCCCGTAACGCCAGGTTGCGCGCCAGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001524 Glycoside hydrolase, family 6, conserved site (Conserved_site)
[147-156] PS00656
PS00656   GLYCOSYL_HYDROL_F6_2
IPR016288 1, 4-beta cellobiohydrolase (Family)
[42-348] 2.20000000000002e-79 G3DSA:3.20.20.40
G3DSA:3.20.20.40   Glyco_hydro_6
[63-82] 3.49999466863949e-38 PR00733 [102-115] 3.49999466863949e-38 PR00733 [130-145] 3.49999466863949e-38 PR00733 [187-200] 3.49999466863949e-38 PR00733 [218-228] 3.49999466863949e-38 PR00733 [248-261] 3.49999466863949e-38 PR00733 [287-307] 3.49999466863949e-38 PR00733 [311-325] 3.49999466863949e-38 PR00733
PR00733   GLHYDRLASE6
[35-353] 8.99992502689852e-84 SSF51989
SSF51989   Glyco_hydro_6
[46-338] 7.90000000000016e-78 PF01341
PF01341   Glyco_hydro_6
[1-352] PIRSF001100
PIRSF001100   Beta_cellobiohydrolase
SignalP
[1-32] 0.999 Signal
Eukaryota   
[1-36] 0.557 Signal
Bacteria, Gram-positive   
[1-35] 0.57 Signal
Bacteria, Gram-negative   
TMHMM
[13-35] Transmembrane (i-o)
Transmembrane 1   
Nanch_00320 Nanch_00320   last last
Page top