Nidda_00070 : CDS information

Location

Organism	Streptomyces caelestis
Strain	NRRL 2821
Entry name	Niddamycin
Contig	AF016585
Start / Stop / Direction	40,626 / 41,097 / + [in whole cluster] 40,626 / 41,097 / + [in contig]
Location	40626..41097 [in whole cluster] 40626..41097 [in contig]
Type	CDS
Length	472 bp (156 aa)

Annotation

Category	3.2 modification methylation
Product	putative methyltransferase(fragment)
Product (GenBank)	N-methyltransferase homolog
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	orf7; tylM1 homolog
Reference	ACC O30769 PmId [9393718] Identification and characterization of the niddamycin polyketide synthase genes from Streptomyces caelestis. (J Bacteriol. , 1997) comment ORF7: N-methyltransferase(N-terminal portion) ORF7は、tylosin生合成clusterのtylM1がコードする領域のN-methyltransferaseであるORF3の産物にN末AA相同性がある。 ORF3もORF7と同じようにPKSの下流に同じ向きで並んでいる。このORFのstart codonはORF5(Nidda_00060)のstop codonの11bp上流から始まるので、これらは翻訳共役かもしれない。
Related Reference	ACC Q9ZGH6 NITE Pikro_00100 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [12119032] Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine. (Biochemistry. , 2002) comment Blast 12th, id60%, 3e-42, N末hit Streptomyces venezuelae_desVI N,N-dimethyltransferase --- [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 DesVI: N,N-Dimethyltransferase desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。 desVI のDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。それらのaglyconeである10-deoxymethynolide and narbonolideを蓄積。 --- [PMID: 12119032](2002)abstract N,N-dimethyltransferasesであるtylM1 and desVI の過剰発現、精製、触媒機能確認。 TylM1 and DesVI はhomodimeric proteinで、ほとんど同じ生化学的特性を持つ。

Sequence

>putative methyltransferase(fragment) [N-methyltransferase homolog]
MYTDEIADVYDLVHEGKGKDYRREAEEIAALVRAERADAGSLLDVACGTGQHLRHLAGLF
DHVEGLELSPDMLRLAARRNPGVPLHQGDMRTFGLGRRFDAVVCMFSSIGHLRDTDELDA
TLERFAQHLGPGGVILVEPWWFPSRSPPVTSGRVTEA-

>putative methyltransferase(fragment) [N-methyltransferase homolog]
ATGTACACCGATGAGATCGCGGACGTCTACGACCTGGTGCACGAGGGGAAGGGCAAGGAC
TACCGGCGTGAGGCGGAGGAGATCGCCGCGCTGGTGCGGGCCGAGCGCGCGGACGCCGGA
TCGCTGCTCGACGTGGCCTGCGGGACCGGTCAGCACCTGCGTCACCTCGCGGGTCTCTTC
GACCACGTGGAAGGCCTCGAGCTCTCCCCCGACATGCTCCGCCTGGCGGCGCGCCGCAAC
CCCGGCGTGCCGCTGCACCAGGGCGACATGCGCACGTTCGGGCTCGGCCGGCGGTTCGAC
GCCGTGGTCTGCATGTTCAGTTCCATCGGTCACCTCCGTGACACCGACGAACTCGACGCC
ACGCTGGAGCGTTTCGCTCAGCACCTCGGCCCGGGCGGCGTGATCCTCGTCGAACCCTGG
TGGTTCCCGAGTCGTTCACCCCCGGTCACGTCGGGGCGAGTTACCGAGGCGG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Nidda_00060 last last