Nysta_00130 : CDS information

Location

Organism	Streptomyces noursei
Strain	ATCC 11455 (=NBRC 15452)
Entry name	Nystatin
Contig	AF263912
Start / Stop / Direction	62,659 / 66,759 / + [in whole cluster] 62,659 / 66,759 / + [in contig]
Location	62659..66759 [in whole cluster] 62659..66759 [in contig]
Type	CDS
Length	4,101 bp (1,366 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	NysA
Gene
Gene (GenBank)	nysA
EC number
Keyword
Note
Note (GenBank)	polyketide synthase; contains KS domain with Cys->Ser substitution in the active site
Reference	ACC Q9L4W5 PmId [10873841] Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. (Chem Biol. , 2000) [21821762] Initiation of polyene macrolide biosynthesis: interplay between polyketide synthase domains and modules as revealed via domain swapping, mutagenesis, and heterologous complementation. (Appl Environ Microbiol. , 2011) comment [PMID: 10873841](2000) Nystatin(nys) biosynthetic gene clusterの報告。 nysA: Type I PKS @Nystatin PKS(loading module) active siteがCys→Ser置換されている。 -- [PMID:21821762](2011) NysAのKS-like domainのactive siteの残基はAT0 domainの機能に影響せず、KSQ domainとは異なる。 AT0 domainの基質特異性はゆるい。

PKS/NRPS Module

acetyl-CoA

KS	8..380

AT	527..838

dh	882..1049

ACP	1213..1283

Sequence

>polyketide synthase [NysA]
MTIGADEDPVVVVGMACRYPGGVAGPEDLWELVRTGRDATTAFPDDRGWDLAALAGDGPG
RSATREGGFLTGAADFDAAFFGMSPREAVSTDPQQRLVLETAWEALERAGIDPHSLRGSR
TGVFVGASGQDYAAVTHASPDDLDGHALTGLAPGVASGRLAYVLGLEGPAVTVDTTSSSS
LVALHWAVRALRAGECSTALAGGVTVMSTPAAFVGHTRQGGLAPDGRCKPFSDDADGTAW
AEGVGIVVLEHLSTARAAGNPVLAVLRGSAVNQDGASDGLTAPSGPAQERVIRAALADAR
LAPADIDLVEAHGTGTRLGDPVEARALLAAYGQDRDPDRPLRLGSLKSTLGHAQAAAGIG
GVIKTVLTLRHGLMPRIRHLATPTRQVDWSQGAVAPLTDHTPWPPADRPRRAGVSSFGIS
GTNAHVILEEAPPADVPVTRPGTLRPSTVPWPVSAATPEALDAQLARLRAHLRTHSDLDP
LDVGYSLATGRAALRHRAVLLPPADGTAADAVEHARGAAHQRRTAVLFSGQGSQRPGMGR
ELAARFPVFADALDDALRALDRHLDGPVREVMWGTDAALLDRTGWTQPALFAVEVALHRL
VASLGVTPDFVGGHSVGEIAAAHVAGVLSLEDACRLVAARATLMQALPAGGAMAALEATE
DEVAPLLGAHLALAAVNGPTAVVVAGAEDAVRQLTARFADRGRRTSRLAVSHAFHSPLME
PMLDAFRDVVSRLTFHQPSIPLVSNLTGELAGSEITSAEYWVRHVRDTVRFADGITALAK
AGADVLIELGPGGVLSAMARDTLGPDSTTDVVPALSKGRPEETAFAGALGRLHTLGVPVD
WPAFYAGTGARRVELPTYAFQHVRHWPTPPRPNGAGPGALGHPLLGSAVELADGGGTVCS
GALSLRTHPWLADHTVAGRVVLPATALLELAVRAGDEAGCDVLHELHLTTPPALPDDAAL
HVQVHVGPADTTGRRAVTVHTRPDHHPAGDWTRCATGTLGSTPPSAAEAATGGTPAAWPP
ADAEPLDLADHYERLADRGFDYGPTFRGLRAAWRRGAEIFADVECPPGTADDAPDHGLHP
ALLDAARHAAMAVDGTVPVAWHGVRLHAVGATALRVRIRPTTTGTLTLTAVDVHGAPVVT
VEALTARPLTDEERAAPRTPRQARGETPADARPARPAAARPGPAGEPLPDTTGSHPTAGH
LAALPPAARERQLLDLVRTQAAAVLGHPGPEAVGTRSVFKELGFDSLAGVELADRLTART
GLRLPATLVFNFPTPERAAHRLGELLAATAPLDPGAYGEELTRFEAIVTNLPQDGPERRA
VADRLDAIVSALRQNSPAEVPSSDEDIDTVSVDRLLDIIDEEFETT

>polyketide synthase [NysA]
ATGACGATCGGAGCCGACGAGGACCCGGTGGTGGTCGTCGGAATGGCCTGCCGTTATCCG
GGTGGGGTCGCCGGCCCGGAGGACCTGTGGGAACTGGTCCGCACCGGCCGCGACGCGACC
ACCGCCTTCCCGGACGACCGCGGCTGGGACCTGGCCGCACTGGCCGGCGACGGACCCGGC
CGCAGCGCGACCCGCGAGGGCGGATTCCTCACCGGCGCCGCCGACTTCGACGCCGCCTTC
TTCGGCATGTCGCCCCGCGAGGCCGTCTCCACCGACCCGCAACAGCGCCTCGTCCTGGAG
ACCGCCTGGGAAGCCCTGGAGCGCGCCGGCATCGACCCGCACTCCCTGCGCGGCAGCCGC
ACCGGGGTCTTCGTCGGCGCCAGCGGCCAGGACTACGCCGCCGTCACCCACGCCTCGCCC
GACGACCTGGACGGACACGCCCTCACCGGCCTGGCCCCCGGCGTCGCCTCCGGTCGCCTG
GCGTACGTCCTGGGCCTCGAAGGCCCCGCCGTCACCGTCGACACCACGTCCTCCTCGTCG
CTGGTCGCGCTGCACTGGGCGGTCCGCGCCCTGCGCGCGGGGGAGTGCAGCACCGCCCTG
GCCGGCGGCGTCACGGTGATGTCCACCCCGGCCGCCTTCGTCGGCCACACCCGACAGGGC
GGCCTCGCGCCCGACGGCCGCTGCAAGCCGTTCTCCGACGACGCCGACGGCACCGCCTGG
GCGGAGGGCGTCGGCATCGTCGTCCTGGAGCACCTGTCCACCGCCCGCGCCGCCGGCAAC
CCCGTCCTCGCCGTGCTGCGCGGCTCGGCCGTCAACCAGGACGGCGCCTCCGACGGCCTC
ACCGCACCCAGCGGTCCCGCCCAGGAACGCGTCATCCGCGCCGCCCTCGCCGACGCCCGA
CTCGCCCCCGCCGACATCGATCTCGTCGAGGCGCACGGCACCGGCACCCGGCTCGGCGAC
CCCGTCGAGGCCCGGGCGCTGCTCGCCGCCTACGGCCAGGACCGGGACCCGGACCGACCG
CTGCGCCTCGGTTCCCTGAAGTCCACCCTCGGCCACGCACAGGCCGCCGCCGGCATCGGC
GGAGTGATCAAGACCGTCCTGACCCTGCGGCACGGCCTGATGCCGCGCATCCGGCACCTG
GCCACCCCCACCCGCCAAGTCGACTGGTCCCAGGGCGCCGTGGCCCCCCTCACCGACCAC
ACGCCCTGGCCACCGGCCGACCGACCGCGCCGCGCCGGCGTCTCCTCCTTCGGCATCAGC
GGCACCAACGCCCATGTGATCCTCGAAGAGGCGCCGCCCGCCGACGTCCCCGTCACCCGG
CCCGGCACCCTCCGCCCCAGCACCGTCCCCTGGCCGGTCTCCGCCGCCACGCCCGAAGCC
CTCGACGCCCAACTCGCCCGGCTCCGCGCCCACCTGCGCACCCACTCGGACCTGGACCCG
CTGGACGTCGGCTACTCCCTGGCCACCGGCCGCGCCGCGCTCCGCCACCGGGCGGTCCTC
CTGCCGCCCGCCGACGGCACCGCCGCGGACGCCGTCGAGCACGCCCGCGGTGCGGCCCAC
CAGCGCCGCACCGCCGTCCTCTTCTCCGGCCAGGGCAGCCAGCGCCCGGGCATGGGCCGC
GAACTCGCCGCCCGCTTCCCGGTGTTCGCCGACGCACTGGACGACGCGCTGCGCGCCCTG
GACCGGCACCTGGACGGCCCGGTGCGCGAGGTGATGTGGGGCACCGACGCCGCGCTCCTG
GACCGGACCGGCTGGACCCAGCCCGCCCTGTTCGCCGTCGAGGTCGCCCTCCACCGCCTG
GTCGCGTCCCTCGGCGTCACCCCCGACTTCGTCGGCGGCCACTCCGTCGGCGAGATCGCC
GCCGCCCACGTCGCCGGCGTCCTGAGCCTGGAGGACGCCTGCCGCCTGGTGGCCGCCCGC
GCCACGCTGATGCAGGCGCTCCCGGCCGGCGGCGCGATGGCCGCGCTGGAGGCCACCGAG
GACGAAGTGGCCCCGCTGCTCGGCGCACACCTCGCGCTGGCCGCCGTCAACGGCCCCACC
GCGGTCGTCGTCGCCGGAGCCGAGGACGCCGTGCGGCAACTGACCGCCCGCTTCGCCGAC
CGCGGCCGGCGCACCAGCCGGCTGGCCGTCTCGCACGCCTTCCACTCGCCGCTGATGGAG
CCCATGCTCGACGCCTTCCGGGACGTCGTGAGCCGACTGACCTTCCACCAGCCGTCGATC
CCGCTGGTCTCCAACCTCACCGGTGAACTCGCCGGCAGTGAGATCACCAGCGCCGAGTAC
TGGGTCCGGCACGTCCGCGACACCGTCCGCTTCGCCGACGGCATCACCGCACTGGCCAAG
GCCGGCGCCGACGTCCTGATCGAACTCGGCCCCGGCGGCGTGCTGTCCGCGATGGCCCGC
GACACCCTCGGCCCCGACAGCACCACCGACGTCGTCCCCGCCCTGAGCAAGGGACGGCCC
GAGGAGACCGCCTTCGCCGGCGCCCTCGGCCGCCTGCACACCCTCGGCGTCCCCGTCGAC
TGGCCCGCCTTCTACGCCGGCACCGGCGCCCGCCGCGTCGAACTGCCCACCTACGCCTTC
CAGCACGTGCGCCACTGGCCCACCCCGCCCCGCCCGAACGGCGCCGGGCCCGGCGCCCTC
GGCCACCCCCTGCTCGGCTCCGCCGTCGAACTCGCCGACGGCGGCGGCACCGTCTGCTCC
GGCGCCCTCTCCCTCCGCACCCACCCCTGGCTCGCCGACCACACCGTCGCCGGGCGGGTC
GTGCTGCCGGCCACCGCGCTGCTGGAACTCGCCGTGCGCGCCGGCGACGAGGCGGGCTGC
GACGTCCTGCACGAACTCCACCTCACCACCCCGCCGGCCCTGCCCGACGACGCCGCCCTG
CACGTCCAGGTGCACGTCGGCCCCGCCGACACCACCGGGCGCCGCGCCGTCACCGTCCAC
ACCCGCCCCGACCACCACCCGGCCGGCGACTGGACCCGATGCGCCACCGGCACCCTCGGC
AGCACCCCGCCGTCCGCAGCCGAAGCCGCCACGGGCGGCACCCCGGCCGCCTGGCCGCCG
GCCGACGCCGAACCCCTCGACCTCGCCGACCACTACGAACGGCTCGCCGACCGCGGCTTC
GACTACGGCCCGACCTTCCGCGGCCTGCGGGCCGCCTGGCGACGCGGCGCGGAGATCTTC
GCGGACGTGGAATGCCCGCCCGGCACCGCCGACGACGCCCCCGACCACGGACTGCACCCC
GCCCTGCTCGACGCGGCCCGGCACGCCGCCATGGCGGTGGACGGCACCGTGCCCGTCGCC
TGGCACGGCGTCCGGCTGCACGCCGTCGGCGCCACCGCGCTGCGGGTCCGCATCCGCCCC
ACCACGACCGGCACGCTGACCCTCACCGCGGTCGACGTGCACGGCGCGCCGGTCGTCACC
GTCGAGGCCCTCACCGCCCGCCCGCTGACCGACGAGGAACGCGCCGCCCCGCGGACGCCG
CGGCAGGCCCGCGGCGAGACGCCCGCCGACGCCCGCCCGGCCCGGCCCGCGGCGGCCCGC
CCCGGCCCGGCCGGCGAACCCCTCCCGGACACCACCGGGTCCCACCCCACCGCCGGCCAC
CTCGCCGCGCTGCCGCCGGCCGCCCGGGAGCGCCAGCTGCTGGACCTGGTGCGCACCCAG
GCCGCCGCCGTCCTGGGCCACCCCGGCCCCGAGGCCGTCGGCACCCGCAGCGTCTTCAAG
GAGCTGGGCTTCGACTCGTTGGCCGGCGTCGAACTCGCCGACCGGCTCACCGCCCGCACC
GGACTGCGCCTGCCGGCCACCCTCGTCTTCAACTTCCCCACCCCCGAACGTGCTGCCCAC
CGCCTCGGGGAACTCCTCGCCGCAACCGCCCCCCTCGACCCCGGGGCGTACGGAGAGGAA
CTCACCAGGTTCGAGGCGATCGTGACGAACCTGCCGCAGGACGGCCCCGAACGCCGGGCC
GTCGCGGACCGGTTGGACGCCATCGTCTCCGCACTCCGCCAGAACTCGCCTGCAGAGGTG
CCCTCCTCGGACGAGGACATCGACACGGTGTCGGTCGACAGACTGCTCGACATCATCGAT
GAAGAGTTCGAAACCACATAG

[0] KS	8..380

[0] AT	527..838

[0] acetyl-CoA	712..716

[0] dh	882..1049

[0] ACP	1213..1283

[0] KS	22..1140

[0] AT	1579..2514

[0] acetyl-CoA	2134..2148

[0] dh	2644..3147

[0] ACP	3637..3849

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) SSF47336 ACP_like PF00550 PP-binding PS50075 ACP_DOMAIN G3DSA:1.10.1200.10 ACP_like IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020807 Polyketide synthase, dehydratase domain (Domain) SM00826 PKS_DH IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS
SignalP	Eukaryota
TMHMM	No significant hit

Nysta_00120 Nysta_00140