Nysta_00220 : CDS information

Location

Organism	Streptomyces noursei
Strain	ATCC 11455 (=NBRC 15452)
Entry name	Nystatin
Contig	AF263912
Start / Stop / Direction	123,468 / 122,404 / - [in whole cluster] 123,468 / 122,404 / - [in contig]
Location	complement(122404..123468) [in whole cluster] complement(122404..123468) [in contig]
Type	CDS
Length	1,065 bp (354 aa)

Annotation

Category	5.1 general function
Product	putative transcriptional regulator
Product (GenBank)	ORF2
Gene
Gene (GenBank)
EC number
Keyword
Note	This ORF is not involved in nystatin biosynthesis.
Note (GenBank)	putative transcriptional regulator
Reference	ACC Q9L4V6 PmId [10873841] Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. (Chem Biol. , 2000) [14973031] In vivo analysis of the regulatory genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455 reveals their differential control over antibiotic biosynthesis. (J Bacteriol. , 2004) comment [PMID: 10873841](2000) Nystatin(nys) biosynthetic gene clusterの報告。 ORF2: transcriptional activator @regulation ORF2はAsnC familyのtranscriptional activatorsに似ている。 nystatin 合成の調節に関与するのかも。 --- [PMID: 14973031](2004) ORF3, ORF2は不活化してもnystatin生合成に影響しなかったので、直接的な調節はしないと示唆される。
Family/Domain	FD IPR019888 comment [IPR019888] Transcription regulator AsnC-type (Family) Molecular Function GO:0043565 sequence-specific DNA binding GO:0003700 transcription factor activity, sequence-specific DNA binding

Sequence

>putative transcriptional regulator [ORF2]
MAQDSGQTPRSLDHVDQALVHALQITPRASWTRIGSVLGLDAVTVARRWNRLVETGAAWI
SCHPAPVLAASGQGCLAFVEIDCAPGRLLDVARALAAAPHVVALSHVTGDRDLQLNVMAR
DPAMLSRWVTHDLAALDGVRAARTHLAGPVHTEGSRWRLRALGRHQVARLAADASRHRTD
TPAFVLDELDQQLVTALSVDGRATYRALAEQCGAGPDTVRRRVQRLFAADMLHARCEVAR
PLSEWPVTVSFWGQVPAARLREVTRRVTGMREVRLCASVISRHNLHLVAWVRSLDDAQRF
EVRLAERAADLTVTERAVALWHMKHGGHLLDEEGYRVGVTPLALWREPTDARRG

>putative transcriptional regulator [ORF2]
GTGGCGCAGGATTCCGGGCAGACCCCCCGGTCCCTGGACCATGTGGATCAGGCCCTGGTG
CACGCGCTGCAGATCACGCCGCGGGCCAGCTGGACCCGGATCGGCTCCGTCCTCGGACTG
GACGCCGTGACGGTGGCCAGGCGCTGGAACCGGCTGGTCGAGACCGGCGCCGCCTGGATC
AGCTGCCATCCGGCCCCGGTGCTGGCCGCGTCGGGCCAGGGGTGCCTGGCGTTCGTCGAG
ATCGACTGCGCTCCCGGCCGACTGCTCGACGTCGCGCGGGCGTTGGCCGCCGCGCCGCAC
GTGGTGGCGCTCTCCCACGTCACCGGGGACCGTGACCTCCAACTCAACGTCATGGCCCGC
GATCCGGCCATGCTCTCCCGTTGGGTGACCCACGACCTCGCCGCGTTGGACGGCGTCCGG
GCCGCCCGGACGCACCTGGCCGGCCCGGTGCACACCGAGGGCAGCCGGTGGCGCCTGCGG
GCCCTCGGCCGGCACCAGGTCGCCCGGCTCGCCGCCGACGCGTCCAGACACCGCACGGAC
ACCCCCGCCTTCGTCCTCGACGAGCTCGACCAACAGCTGGTCACCGCGCTGTCCGTGGAC
GGCCGGGCCACCTACCGCGCGCTGGCCGAGCAGTGCGGCGCCGGCCCCGACACCGTGCGC
CGTCGCGTCCAACGGCTCTTCGCCGCGGACATGTTGCACGCCCGGTGCGAGGTCGCCCGC
CCGCTGTCCGAGTGGCCGGTCACGGTGAGCTTCTGGGGGCAGGTCCCGGCCGCGAGGCTG
CGCGAGGTGACCCGCCGGGTCACCGGGATGCGCGAGGTCCGCCTGTGCGCGAGCGTCATC
AGCCGGCACAACCTGCACCTGGTGGCCTGGGTGCGCTCCCTCGACGACGCCCAGCGCTTC
GAGGTCCGACTCGCCGAACGGGCCGCGGACCTGACCGTCACCGAACGCGCGGTCGCCCTC
TGGCACATGAAGCACGGCGGCCATCTCCTCGACGAGGAGGGCTACCGGGTGGGGGTGACA
CCGCTCGCACTCTGGCGGGAACCGACGGACGCGCGACGCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000485 HTH transcription regulator AsnC-type DNA-binding domain (Domain) PR00033 HTHASNC IPR011008 Dimeric alpha-beta barrel (Domain) SSF54909 Dimer_A_B_barrel IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR019887 Transcription regulator AsnC-type, C-terminal (Domain) G3DSA:3.30.70.920 G3DSA:3.30.70.920 PF01037 AsnC_trans_reg IPR019888 Transcription regulator AsnC-type (Family) SM00344 HTH_ASNC
SignalP	No significant hit
TMHMM	No significant hit

Nysta_00210 last last