Oligo_00010 Oligo_00010   Oligo_00030 Oligo_00030

Oligo_00020 : CDS information

close this sectionLocation

Organism
StrainATCC 31267 (=NBRC 14893)
Entry nameOligomycin
Contig
Start / Stop / Direction1,886 / 1,131 / - [in whole cluster]
1,886 / 1,131 / - [in contig]
Locationcomplement(1131..1886) [in whole cluster]
complement(1131..1886) [in contig]
TypeCDS
Length756 bp (251 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative phosphopantetheinyl transferase
Product (GenBank)phosphopantheteinyl transferase
Gene
Gene (GenBank)pptA
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11572948] Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. (Proc Natl Acad Sci U S A. , 2001)
comment
S. avermitilisのゲノムの99%をカバーしたシークエンス解析の報告。
そこで見つかった二次代謝に関するclusterについて述べている。

このORFについての記載は特にされていない。
figにそれらしきORFはあるがコメントなし。
Related Reference
ACC
Q6T709
PmId
[14695521] Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in Saccharopolyspora erythraea. (Chembiochem. , 2004)
comment
5th, id52%, 3e-50
Saccharopolyspora erythraea_pptII
Sfp-type phosphopantetheinyl transferase

3つのPPTase genes
ACPS-type PPTase (S. erythraea ACPS)
Sfp-type PPTases (SePptI, SePptII)あるが、
erythromycin PKSのacyl carrier protein-thioesterase didomainに対してPPTase活性あったのはSePptIIのみ(in vitro)。
ACC
Q9F0Q6
PmId
[11451672] Cloning and characterization of a phosphopantetheinyl transferase from Streptomyces verticillus ATCC15003, the producer of the hybrid peptide-polyketide antitumor drug bleomycin. (Chem Biol. , 2001)
comment
65th, id53%, 1e-36
Streptomyces verticillus_pptA
Phosphopantetheinyl transferase PptA

E. coli発現、精製後PPTase活性あり(基質: S. verticillus, 他Streptomycesのacyl carrier proteins(ACP)(type I,II), peptidyl carrier proteins(PCP))。
特異性は、基質BlmI PCP(Q9XC48) > TcmM ACP

遺伝子はpolyketides, nonribosomal peptides合成gene clusterには含まれていない(cluster内に別PPTase gene含まない)。

PPTase subfamily(ACPS-type or Sfp-type)は、Sfp-type。

close this sectionSequence

selected fasta
>putative phosphopantetheinyl transferase [phosphopantheteinyl transferase]
MRAHEVFADVPVSSLHPREAALVQDAVESRRREFATGRHCARRALASLGVPPGPLLATPR
GAPRWPAGVCGAITHCAGYRAAAVARAPGIRAVGIDAEPDRPLPEGVLEAVALPAEQFQV
KEHTARHPGVVWGKLLFSAKESVYKAWFPATGRPLGFEEALIDLDPVSSTFRVRVLPTAL
RAAEPELRDLHGRWLVRHGIIVTAVVVDGPGRDTPHAPSPYDRTKHSAPTGRSADRPGGR
PTDRATDRPTA
selected fasta
>putative phosphopantetheinyl transferase [phosphopantheteinyl transferase]
GTGCGGGCGCACGAGGTCTTCGCCGACGTCCCGGTCTCCTCGCTGCACCCCCGGGAGGCC
GCCCTCGTCCAGGACGCCGTGGAGAGCCGCCGCCGGGAGTTCGCGACCGGCCGGCACTGC
GCACGCCGGGCACTGGCCTCGCTCGGCGTCCCGCCCGGCCCGCTGCTCGCCACCCCGAGG
GGCGCACCCCGCTGGCCCGCCGGTGTGTGCGGAGCGATCACCCACTGCGCCGGGTACCGC
GCCGCCGCCGTCGCCCGCGCCCCCGGCATCAGGGCCGTCGGCATCGACGCGGAGCCCGAC
CGGCCGCTTCCGGAGGGAGTCCTGGAGGCCGTCGCGCTGCCCGCGGAGCAGTTCCAGGTC
AAGGAGCACACGGCCCGGCACCCCGGTGTCGTCTGGGGAAAGCTGCTGTTCAGCGCGAAG
GAGAGCGTCTACAAGGCGTGGTTCCCGGCGACCGGGCGGCCCCTGGGCTTCGAGGAGGCC
CTGATCGACCTCGACCCCGTCAGCAGCACCTTCCGCGTACGGGTGCTCCCCACCGCCCTG
CGCGCGGCCGAGCCGGAGCTGCGGGACCTTCACGGCCGCTGGCTCGTCCGGCACGGGATC
ATCGTCACGGCGGTCGTGGTGGACGGCCCCGGCCGGGACACACCGCACGCCCCGTCGCCG
TACGACCGTACGAAGCACTCCGCGCCCACCGGCCGTTCCGCCGACCGGCCCGGCGGCCGG
CCCACCGACCGCGCCACCGACCGCCCCACCGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003542 Enterobactin synthetase-like, component D (Family)
[28-48] 7.40000780398343e-23 PR01399 [58-76] 7.40000780398343e-23 PR01399 [135-151] 7.40000780398343e-23 PR01399
PR01399   ENTSNTHTASED
IPR008278 4'-phosphopantetheinyl transferase (Domain)
[90-166] 9.9e-07 G3DSA:3.90.470.20
G3DSA:3.90.470.20   G3DSA:3.90.470.20
[89-209] 1.49999977583352e-14 SSF56214
SSF56214   4-PPT_transf
[93-168] 9.7e-11 PF01648
PF01648   ACPS
SignalP No significant hit
TMHMM No significant hit
Oligo_00010 Oligo_00010   Oligo_00030 Oligo_00030
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