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Oligo_00180 : CDS information

close this sectionLocation

Organism
StrainATCC 31267 (=NBRC 14893)
Entry nameOligomycin
Contig
Start / Stop / Direction103,501 / 104,319 / + [in whole cluster]
103,501 / 104,319 / + [in contig]
Location103501..104319 [in whole cluster]
103501..104319 [in contig]
TypeCDS
Length819 bp (272 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative 3-hydroxyacyl-CoA dehydrogenase
putative beta-keto-reductase
Product (GenBank)3-hydroxyacyl-CoA dehydrogenase
Gene
Gene (GenBank)hbdA
EC number1.1.1.35
Keyword
  • ethylmalonyl-CoA
Note
Note (GenBank)
Reference
ACC
PmId
[11572948] Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. (Proc Natl Acad Sci U S A. , 2001)
comment
S. avermitilisのゲノムの99%をカバーしたシークエンス解析の報告。
そこで見つかった二次代謝に関するclusterについて述べている。

このORFについての記載は特にされていない。
figにそれらしきORFはあるがコメントなし。
Related Reference
ACC
P52041
PmId
[8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996)
comment
278th, 43%, 2e-53
Clostridium acetobutylicum_hbd
3-hydroxybutyryl-CoA dehydrogenase(EC 1.1.1.157)

BCS (butyryl-CoA synthesis) operon (crt-bcd-etfB-etfA-hbd)のクローニングとシークエンス
crt geneの上流にあるプロモーターによって制御されるtranscriptional unitであることを確認

BCS operonをE. coliで大量発現。crotonase, beta-hydroxybutyryl-CoA dehydrogenase activity確認

BCS operonをC. acetobutylicum で発現。crotonase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase activity上昇

close this sectionSequence

selected fasta
>putative 3-hydroxyacyl-CoA dehydrogenase [3-hydroxyacyl-CoA dehydrogenase]
MMGAGLAEVCARAGLDVLVTSSGEDGLARGRGRLERSLAHAVRKDRISDTAREETLARVS
FTTDLAAFRDRQLVLEAAPEDEPTKLRIFQALDRAVEDPEAILATNTSALPVMRLARATD
RPGQVLGLHFFNPAPVLPLVEVIGSLLTRDRTRRIAAEFATTVLGKQVVHAGDRSGFVVN
ALLIPYLLSAIRMCEAGFAGAQDIDKAMTLGCSHPMGPLALADLIGLDTVAAIGTALYEE
FKEPTHAVPPLLSRMVDGGLLGRKSGQGFYTY
selected fasta
>putative 3-hydroxyacyl-CoA dehydrogenase [3-hydroxyacyl-CoA dehydrogenase]
GTGATGGGCGCGGGCCTCGCCGAAGTGTGCGCCCGGGCCGGGCTCGACGTACTGGTCACG
TCCTCGGGCGAGGACGGGCTGGCCCGGGGCCGCGGCCGGCTCGAACGCTCCCTCGCCCAC
GCCGTCCGCAAGGACAGGATCAGCGACACGGCCCGCGAAGAGACGCTCGCCCGCGTCTCC
TTCACCACCGACCTCGCCGCCTTCCGCGACCGGCAACTGGTCCTGGAGGCGGCCCCCGAG
GACGAGCCCACGAAGCTGCGGATCTTCCAGGCGCTGGACAGGGCCGTCGAGGACCCGGAG
GCGATCCTCGCCACCAACACCTCCGCCCTTCCCGTCATGCGGCTGGCCCGCGCGACCGAC
CGCCCCGGCCAGGTCCTCGGCCTGCACTTCTTCAACCCGGCCCCCGTGCTGCCCCTGGTC
GAGGTGATCGGTTCGCTGCTCACCCGCGACCGCACCCGGCGCATCGCCGCGGAGTTCGCC
ACCACGGTGCTCGGCAAGCAGGTCGTCCACGCCGGCGACCGCTCCGGGTTCGTGGTCAAC
GCCCTGCTGATCCCCTATCTCCTGTCCGCGATCAGGATGTGCGAGGCGGGATTCGCCGGC
GCGCAGGACATCGACAAAGCGATGACACTGGGCTGCTCGCACCCCATGGGACCGCTGGCA
CTGGCCGACCTCATCGGCCTGGACACCGTCGCGGCCATCGGCACGGCCCTGTACGAGGAG
TTCAAGGAGCCCACGCACGCCGTTCCCCCGCTGCTGTCCCGCATGGTCGACGGCGGCCTG
CTCGGCAGGAAGTCCGGGCAGGGTTTCTACACGTACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
[176-272] 9.29999999999998e-30 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
[1-173] 5.30000000000001e-48 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
[176-272] 9.00000407957274e-30 SSF48179
SSF48179   6DGDH_C_like
IPR013328 Dehydrogenase, multihelical (Domain)
[178-272] 1.8e-31 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   Opine_DH
IPR016040 NAD(P)-binding domain (Domain)
[1-177] 2.59999999999998e-40 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family)
[1-272] 5.29999657550189e-32 PIRSF000105
PIRSF000105   HCDH
SignalP No significant hit
TMHMM No significant hit
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