Rapam_00010 : CDS information

Location

Organism	Streptomyces rapamycinicus
Strain	NRRL 5491
Entry name	Rapamycin
Contig	X86780
Start / Stop / Direction	871 / 1 / - [in whole cluster] 871 / 1 / - [in contig]
Location	complement(1..871) [in whole cluster] complement(1..871) [in contig]
Type	CDS
Length	871 bp (289 aa)

Annotation

Category	4.3 transport
Product	putative transporter(fragment)
Product (GenBank)	monosaccharide transporter
Gene
Gene (GenBank)	orfZZ
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q54286 PmId [7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995) [8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996) comment [PMID: 7644502](1995) rapamycin生合成gene clusterの報告。配列解析。 ORF ZZ: 記載なし --- [PMID: 8635730](1996) PKS flanking genesの配列解析。 glucoseなど糖のproton-linked transportersと似ている。よってここからはrap cluster外と判断している。
Related Reference	ACC P15686 PmId [2599110] The hexose carrier from Chlorella. cDNA cloning of a eucaryotic H+-cotransporter. (FEBS Lett. , 1989) comment 255th(P15686) 31%, 2e-11 Chlorella kessleri_HUP1 H(+)/hexose cotransporter 1 Function: Active uptake of hexoses. Induction by glucose.

Sequence

>putative transporter(fragment) [monosaccharide transporter]
MAPRPSVRSVADVVALIDGLGKITGRAQIIWFLVFGGLFLDAYSNAALSAGLGPMTTQME
LTSTQVSILTATAPALAILFNPLGGWLATRIGRVPPLLIAKLFAIAGALLAAFAGDFTVV
WLGRVLVGVAYGMDFAVAMALLAEYTPAKLGGRLNLWQAVWYIATTSNLVLALVFFNLDV
GADIWRWSVGSAAVVAVALLLGQWLMLKESPTWLAGKGRLGEAITNLDKIYGIKAVAGTP
DEMTRTPAEAPVIGLRQAGRLFRGEYLPRTILSSVISLGQSMQYFAVGWY-

>putative transporter(fragment) [monosaccharide transporter]
ATGGCACCACGTCCGAGCGTCAGAAGCGTCGCCGACGTCGTTGCGCTCATCGATGGGCTG
GGAAAGATCACCGGCCGGGCGCAGATCATCTGGTTCCTCGTGTTCGGCGGGCTGTTCCTC
GACGCCTACTCGAACGCCGCGCTGAGCGCCGGACTTGGGCCCATGACGACCCAGATGGAG
CTCACCAGCACCCAGGTCTCGATCCTCACGGCCACCGCTCCGGCCCTGGCGATCCTCTTC
AACCCCCTCGGCGGCTGGCTCGCCACCCGCATCGGCCGGGTCCCCCCGCTGCTCATCGCC
AAGCTGTTCGCGATCGCGGGTGCCCTGCTCGCCGCCTTCGCAGGTGACTTCACCGTCGTC
TGGCTCGGCCGGGTGCTGGTGGGTGTCGCCTACGGGATGGACTTCGCGGTCGCCATGGCG
CTGCTCGCCGAGTACACCCCCGCGAAGCTGGGCGGTCGGCTCAACCTGTGGCAAGCCGTC
TGGTACATCGCCACCACCAGCAACCTCGTCCTCGCCCTGGTCTTCTTCAACCTGGACGTC
GGCGCGGACATCTGGCGCTGGTCGGTCGGCTCGGCCGCGGTCGTCGCCGTCGCGCTCCTC
CTGGGCCAGTGGCTGATGCTCAAGGAGAGCCCCACCTGGCTGGCCGGCAAGGGCCGCCTG
GGCGAGGCGATCACCAACCTGGACAAGATCTACGGGATCAAGGCCGTCGCCGGTACGCCC
GACGAGATGACCCGCACCCCGGCTGAGGCGCCGGTCATCGGGCTCCGGCAGGCCGGACGG
CTGTTCAGGGGCGAGTACCTGCCGCGTACGATCCTCTCCTCGGTGATCTCGCTCGGCCAG
TCGATGCAGTACTTCGCCGTCGGCTGGTACC

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005828 General substrate transporter (Family) PF00083 Sugar_tr IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain) SSF103473 MFS general substrate transporter IPR020846 Major facilitator superfamily domain (Domain) PS50850 MFS
SignalP	No significant hit
TMHMM	Transmembrane 5

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