Rapam_00090 Rapam_00090   Rapam_00110 Rapam_00110

Rapam_00100 : CDS information

close this sectionLocation

Organism
StrainNRRL 5491
Entry nameRapamycin
Contig
Start / Stop / Direction10,131 / 9,337 / - [in whole cluster]
10,131 / 9,337 / - [in contig]
Locationcomplement(9337..10131) [in whole cluster]
complement(9337..10131) [in contig]
TypeCDS
Length795 bp (264 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)ketoreductase/dehydrogenase
Gene
Gene (GenBank)rapT
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995)
[8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996)
comment
[PMID: 7644502](1995)
rapamycin生合成gene clusterの報告。配列解析。
RapT: ketoreductase/dehydrogenase

rapTはPKSをコードするrapBのすぐ下流に並んでいて、aromatic PKSsやfatty acid synthasesに見られるketoreductasesに明らかな配列類似を持つ酵素をコードする。

---
[PMID: 8635730](1996)
PKS flanking genesの配列解析。
graやmonのようなtype II PKSのbeta-ketoacyl-ACP ketoreductasesに似ている。
dihydroxycyclohexane carboxylic acid starter unitの合成に関連するかもしれない（他論文からの引用）。
Related Reference
ACC
P0AEK2
PmId
[1556094] The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes. (J Biol Chem. , 1992)
[11669613] Structure of beta-ketoacyl-[acyl carrier protein] reductase from Escherichia coli: negative cooperativity and its structural basis. (Biochemistry. , 2001)
comment
254th(P0AEK2) 31%, 4e-27
E.coli_fabG
3-oxoacyl-[acyl-carrier-protein] reductase(EC 1.1.1.100)

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

--
[PMID: 1556094](1992)
acpP-fabD のクローニングとシークエンス, cotranscriprion確認。

--
[PMID: 11669613](2001)
FabGの結晶構造解析。
FabGはNADPHのnegative cooperative bindingを示す。この効果はACPの存在によって拡大される。

close this sectionSequence

selected fasta
>putative oxidoreductase [ketoreductase/dehydrogenase]
MATTLEHRFAGQVALVTGGGSGIGAAVAHLLAAEGCAAVHVGDLNAEGVRTVASVIKGVA
HVVDVTDPRAVDAVVSSVLESEGRIDVVIHTAGVDDPDAKARILEAQESGEPVDVLTRLD
DAAWRRVMAVNLDGTFHVLRAAVRAMRPRGRGAIVTVGSSAAFDTLTGYPHYAASKAGVH
ALSQAVAKEAIAFGIRVNTVAPGPVDTPMAARTPDAVRQKMAESGASGYATPEELADNIC
YLASPAASNVVGAVLLSNGGRFTV
selected fasta
>putative oxidoreductase [ketoreductase/dehydrogenase]
ATGGCAACGACCCTTGAGCACCGCTTCGCGGGACAGGTCGCCCTCGTTACCGGGGGCGGG
TCAGGAATCGGGGCCGCGGTGGCGCACCTGCTCGCTGCCGAAGGATGTGCGGCTGTGCAC
GTGGGCGACCTCAACGCGGAGGGTGTTCGCACCGTCGCGTCCGTCATCAAAGGGGTTGCG
CACGTTGTCGATGTGACCGATCCGCGGGCCGTTGATGCCGTAGTGTCGTCCGTCCTCGAA
TCCGAAGGCCGTATCGACGTGGTCATCCACACGGCCGGGGTGGACGATCCCGACGCCAAG
GCGCGAATCCTCGAGGCTCAGGAGTCGGGCGAGCCGGTGGATGTGCTGACTCGACTCGAT
GACGCCGCCTGGCGCCGGGTGATGGCCGTGAACCTCGACGGTACTTTCCACGTTCTGCGA
GCCGCGGTGCGAGCCATGCGCCCACGCGGCAGGGGCGCCATCGTGACTGTTGGCTCGTCC
GCGGCGTTCGACACGCTCACGGGATATCCGCACTACGCCGCTTCCAAAGCCGGTGTGCAC
GCGCTGTCCCAGGCCGTGGCAAAAGAGGCCATCGCTTTCGGTATCCGCGTCAACACCGTC
GCTCCGGGGCCAGTGGACACACCGATGGCGGCGCGTACCCCGGACGCCGTCCGCCAGAAG
ATGGCCGAGTCGGGCGCGTCGGGATACGCGACGCCGGAGGAGCTCGCCGACAACATCTGC
TATCTGGCGTCGCCTGCCGCGTCCAATGTGGTTGGTGCGGTGCTGCTGAGCAACGGAGGC
CGGTTCACGGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[83-94] 7.49999605851445e-09 PR00080 [152-160] 7.49999605851445e-09 PR00080 [172-191] 7.49999605851445e-09 PR00080
PR00080   SDRFAMILY
[13-189] 1.99999999999999e-25 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[13-30] 3.80000697093588e-36 PR00081 [83-94] 3.80000697093588e-36 PR00081 [146-162] 3.80000697093588e-36 PR00081 [172-191] 3.80000697093588e-36 PR00081 [193-210] 3.80000697093588e-36 PR00081 [225-245] 3.80000697093588e-36 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[8-262] 2.09999999999996e-67 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[159-187] PS00061
PS00061   ADH_SHORT
SignalP
[1-38] 0.832 Signal
Eukaryota   
[1-32] 0.519 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Rapam_00090 Rapam_00090   Rapam_00110 Rapam_00110
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