Rapam_00140 Rapam_00140   Rapam_00160 Rapam_00160

Rapam_00150 : CDS information

close this sectionLocation

Organism
StrainNRRL 5491
Entry nameRapamycin
Contig
Start / Stop / Direction90,798 / 91,433 / + [in whole cluster]
90,798 / 91,433 / + [in contig]
Location90798..91433 [in whole cluster]
90798..91433 [in contig]
TypeCDS
Length636 bp (211 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
ProductC-27 O-methyltransferase
Product (GenBank)methyltransferase
Gene
Gene (GenBank)rapQ
EC number2.1.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995)
[8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996)
[11372782] Deletion of rapQONML from the rapamycin gene cluster of Streptomyces hygroscopicus gives production of the 16-O-desmethyl-27-desmethoxy analog. (J Antibiot (Tokyo). , 2001)
[16990929] Rapamycin biosynthesis: Elucidation of gene product function. (Org Biomol Chem. , 2006)
comment
[PMID: 7644502](1995)
rapamycin生合成gene clusterの報告。配列解析。
RapQ: methyltransferase

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[PMID: 8635730](1996)
PKS flanking genesの配列解析。
three potential SAM-dependent O-methyltransferases (MTases), RapI, RapM and RapQ.
これらはmacrocycleの後期修飾に関与している可能性がある。

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[PMID: 11372782](2001)
rapQONMLのdeletion mutantはpipecolateを添加すると、rapamycinの代わりにanalog 16-O-desmethyl-27-desmethoxyrapamycinを産生した。これは、rapNによってコードされるP450 hydroxylaseがC-27に特異的で、rapQとrapM によってコードされるO-methyltransferasesがC-16 and C-27でのhydroxyl基をメチル化することを示す。

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[PMID: 16990929](2006)
rapKIJMNOQL deletion mutant S.hygroscopicus MG2-10をrapJNOQLで相補して、starterとしてcyclohexanecarboxylic acidを与えると、C-27にmethoxy基のある化合物を産生。
rapKが単独でS.lividans TK24に発現されても、C-27 OH基が形成されないので、pre-rapamycin(未修飾aglycon)は修飾されなかった。
他の遺伝子組み合わせでの相補実験結果もふまえ、RapQはC-27 OH基に特異的なO-methylationを担うと言える。

close this sectionSequence

selected fasta
>C-27 O-methyltransferase [methyltransferase]
MMLELGTRLKFRFTGPLLEAVNPRLQGHPYDVLMRLLEGGRIENVLELCGGTGFASRMLA
ERHSKVQATSIDLSPELTAVGPHKLASRGIDNVTLVEGDVSTLPYPDDSFDTVMSAFGLH
EVPTAGRLSAIRESVRVLKPGGRFVIVDLDRRTKYGWTMDLFMKVMEPKFAPEVFGTGLV
DRLKENGFTIDHHESAGPNGWTQSIVATLEA
selected fasta
>C-27 O-methyltransferase [methyltransferase]
GTGATGTTGGAATTGGGTACCCGCCTGAAGTTCAGATTTACCGGACCGCTCCTCGAAGCT
GTCAATCCGCGCCTGCAGGGTCACCCCTACGACGTACTGATGCGCCTCCTCGAGGGCGGT
CGCATCGAAAACGTGCTGGAATTGTGCGGCGGGACCGGATTCGCGTCGCGAATGCTGGCC
GAAAGGCATTCGAAGGTACAGGCCACCTCCATCGATCTGTCACCCGAACTGACCGCGGTA
GGCCCCCACAAGTTGGCCTCGCGGGGGATCGATAACGTCACCCTGGTCGAGGGTGACGTT
TCCACCTTGCCTTACCCCGACGACTCGTTCGACACCGTGATGTCGGCCTTCGGTCTGCAC
GAGGTCCCGACCGCGGGCCGCCTCAGCGCGATCCGCGAGTCCGTGCGGGTGCTCAAGCCC
GGCGGCCGCTTCGTCATCGTCGATCTCGACCGCCGGACAAAGTACGGGTGGACGATGGAC
CTCTTCATGAAGGTCATGGAGCCGAAGTTCGCCCCCGAGGTGTTCGGAACCGGCCTGGTG
GACCGGCTGAAAGAGAACGGTTTCACCATCGACCATCACGAGTCGGCCGGGCCGAACGGA
TGGACACAGTCGATCGTCGCCACCCTGGAGGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004033 UbiE/COQ5 methyltransferase (Family)
[35-156] 1e-18 PF01209
PF01209   Ubie_methyltran
SignalP No significant hit
TMHMM No significant hit
Rapam_00140 Rapam_00140   Rapam_00160 Rapam_00160
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