Rapam_00160 : CDS information

Location

Organism	Streptomyces rapamycinicus
Strain	NRRL 5491
Entry name	Rapamycin
Contig	X86780
Start / Stop / Direction	91,767 / 91,534 / - [in whole cluster] 91,767 / 91,534 / - [in contig]
Location	complement(91534..91767) [in whole cluster] complement(91534..91767) [in contig]
Type	CDS
Length	234 bp (77 aa)

Annotation

Category	4.5 electron carrier
Product	putative ferredoxin
Product (GenBank)	ferredoxin
Gene
Gene (GenBank)	rapO
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q54301 PmId [7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995) [8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996) comment [PMID: 7644502](1995) rapamycin生合成gene clusterの報告。配列解析。 RapO: ferredoxin 詳細記載なし --- [PMID: 8635730](1996) PKS flanking genesの配列解析。 rapOは[3Fe-4S] clustersを含んだauthentic ferredoxinsに似た確かな配列を持つ小さな酸性タンパクをコード。 CYP450は電子伝達を必要とする。 rapOはCYP450をコードするrapNに隣接、翻訳共役するだろう。もうひとつのCYP450をコードするrapJとの関連は不明。
Related Reference	ACC P29603 PmId [8045914] ( , ) comment Blast list外、id30.16%, 4e-04 Pyrococcus furiosus_fdxA Ferredoxin ferredoxin gene fdxAのクローニング、シークエンス、E.coliで発現して得たrecombinant ferredoxinとnative ferredoxinsの特性を比較して一致を確認している。

Sequence

>putative ferredoxin [ferredoxin]
MSGMARWELWIDRTECIGSAVCVASAGAYFEMHGHQSRARASEIDADEHVIAVAQACPSG
AIHVRVKETGELLEPQD

>putative ferredoxin [ferredoxin]
GTGAGTGGCATGGCGCGATGGGAGCTGTGGATCGACCGGACCGAGTGCATCGGCTCGGCC
GTCTGCGTGGCCAGCGCGGGCGCGTACTTCGAGATGCACGGGCACCAGTCGCGCGCCCGC
GCGAGCGAGATCGACGCGGACGAGCACGTGATCGCGGTGGCGCAGGCCTGCCCGTCGGGG
GCGATCCACGTCCGGGTCAAGGAGACCGGCGAGCTTCTCGAACCCCAGGACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Rapam_00150 Rapam_00170