Rapam_00170 : CDS information

Location

Organism	Streptomyces rapamycinicus
Strain	NRRL 5491
Entry name	Rapamycin
Contig	X86780
Start / Stop / Direction	92,978 / 91,764 / - [in whole cluster] 92,978 / 91,764 / - [in contig]
Location	complement(91764..92978) [in whole cluster] complement(91764..92978) [in contig]
Type	CDS
Length	1,215 bp (404 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450
Product (GenBank)	cytochrome P450
Gene
Gene (GenBank)	rapN
EC number
Keyword	C-27 hydroxylate
Note
Note (GenBank)
Reference	ACC Q54302 PmId [7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995) [8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996) [11372782] Deletion of rapQONML from the rapamycin gene cluster of Streptomyces hygroscopicus gives production of the 16-O-desmethyl-27-desmethoxy analog. (J Antibiot (Tokyo). , 2001) [16990929] Rapamycin biosynthesis: Elucidation of gene product function. (Org Biomol Chem. , 2006) comment [PMID: 7644502](1995) rapamycin生合成gene clusterの報告。配列解析。 RapN: Cytochrome P450 rapJとrapNの遺伝子産物はcytochrome P450 enzymesに似ており、C-9 and C-27 (and possibly also C-26 or C-32)をhydroxylateするかもしれない。 --- [PMID: 8635730](1996) PKS flanking genesの配列解析。 RapJとRapNという2つのcytochrome P-450 monooxygenases (P450s)があり、これらはmacrocycleの'late' modificationに関与している可能性がある。 --- [PMID: 11372782](2001) rapQONMLのdeletion mutantはpipecolateを添加すると、rapamycinの代わりにanalog 16-O-desmethyl-27-desmethoxyrapamycinを産生した。これは、rapNによってコードされるP450 hydroxylaseがC-27に特異的で、rapQとrapM によってコードされるO-methyltransferasesがC-16 and C-27でのhydroxyl基をメチル化することを示す。 -- [PMID: 16990929](2006) rapKIJMNOQL deletion mutant S.hygroscopicus MG2-10の相補実験で、rapNを含んだ株では特異的にC-27がhydroxylationされた産物が得られる。

Sequence

>cytochrome P450 [cytochrome P450]
MSTTDQGETGKACPYPFAEMERLEIHPEYNRLRDAGELGRVLMLDGREAWLAASWEDVAK
VFVDPRFSRSATLGKDVPRVLPAIQDQPVIMLMDPPEHTRLRRVATKALTSRRMEALRPR
TQEVADDLIDKMLAKGAPADLMEDFALPLPIIMICELLGVPIEDQTKFRTWSDQMLSNGA
YSQEVVMAAGQSLYLYLSELIAERRKQDTNDLLGSLVRARDKDDRLSETELVGFAVTLLI
AGYETTANAIGNSVYTLLTHPEKLAELRKDLSLIPKAVDELLRIIPIAKQASWVRMAVED
VELSGTIVKAGEAVAIQTHSANTDPKVYDHPEEIDFHRTSNPHMSLGHGAHHCMGAQLVR
VEMQTALGSLISRIPALRFAVPEPRIKFLRGRLVPSLEALPLTW

>cytochrome P450 [cytochrome P450]
ATGTCGACGACCGATCAGGGTGAGACCGGAAAGGCCTGCCCTTATCCTTTCGCCGAAATG
GAGCGACTCGAGATCCACCCCGAGTACAACCGGCTGCGGGACGCCGGTGAGCTCGGCCGG
GTCCTGATGTTGGACGGCCGGGAGGCCTGGCTGGCCGCCAGCTGGGAGGACGTCGCCAAG
GTGTTCGTGGACCCGCGGTTCAGCCGCAGCGCGACGCTCGGCAAGGACGTGCCCCGGGTC
CTCCCGGCGATCCAGGACCAGCCCGTCATCATGCTGATGGACCCTCCGGAACACACCCGG
CTGCGCCGGGTGGCGACCAAGGCGCTGACCAGCCGCCGGATGGAGGCCCTGCGTCCGCGC
ACCCAGGAGGTCGCCGACGATCTGATCGACAAAATGCTGGCCAAGGGCGCCCCCGCCGAT
CTGATGGAGGACTTCGCCCTGCCTCTGCCGATCATCATGATCTGTGAGCTGCTGGGCGTG
CCGATCGAGGACCAGACCAAGTTCCGGACCTGGTCGGACCAGATGCTCTCGAACGGCGCC
TACTCACAGGAAGTCGTGATGGCGGCCGGCCAGTCGCTCTACCTCTACCTCTCCGAGCTG
ATCGCGGAACGTCGCAAGCAGGACACCAACGATCTGCTCGGGTCGCTGGTGCGGGCCCGC
GACAAGGACGACCGGCTCAGCGAGACGGAGCTGGTCGGCTTCGCCGTCACACTGCTGATA
GCGGGATACGAGACCACCGCCAACGCGATCGGCAACAGCGTCTACACCCTGCTGACCCAC
CCGGAGAAACTCGCCGAACTGCGAAAGGACCTCTCGCTGATCCCGAAGGCGGTCGACGAG
TTGCTCCGGATCATCCCGATCGCCAAGCAGGCGAGCTGGGTCCGGATGGCGGTGGAGGAC
GTCGAGCTCAGCGGCACGATCGTCAAGGCCGGTGAGGCGGTGGCGATCCAGACGCACTCG
GCCAACACCGACCCCAAGGTCTACGACCATCCGGAGGAGATCGACTTCCACCGGACGAGC
AACCCGCACATGTCCCTCGGACACGGCGCGCACCACTGCATGGGCGCTCAACTCGTCCGG
GTGGAGATGCAGACCGCGCTCGGCTCGCTCATCTCCCGTATCCCGGCGCTGCGGTTCGCG
GTGCCGGAGCCGCGGATCAAGTTCCTGCGCGGGCGGCTGGTGCCCAGCCTCGAAGCGTTG
CCCCTGACATGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PR00385 P450 SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450
SignalP	No significant hit
TMHMM	No significant hit

Rapam_00160 Rapam_00180