Rapam_00180 : CDS information

Location

Organism	Streptomyces rapamycinicus
Strain	NRRL 5491
Entry name	Rapamycin
Contig	X86780
Start / Stop / Direction	93,945 / 92,992 / - [in whole cluster] 93,945 / 92,992 / - [in contig]
Location	complement(92992..93945) [in whole cluster] complement(92992..93945) [in contig]
Type	CDS
Length	954 bp (317 aa)

Annotation

Category	3.2 modification methylation
Product	C-16 O-methyltransferase
Product (GenBank)	methyltransferase
Gene
Gene (GenBank)	rapM
EC number	2.1.1.-
Keyword
Note
Note (GenBank)
Reference	ACC Q54303 PmId [7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995) [8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996) [11372782] Deletion of rapQONML from the rapamycin gene cluster of Streptomyces hygroscopicus gives production of the 16-O-desmethyl-27-desmethoxy analog. (J Antibiot (Tokyo). , 2001) [16990929] Rapamycin biosynthesis: Elucidation of gene product function. (Org Biomol Chem. , 2006) comment [PMID: 7644502](1995) rapamycin生合成gene clusterの報告。配列解析。 RapM: methyltransferase --- [PMID: 8635730](1996) PKS flanking genesの配列解析。 three potential SAM-dependent O-methyltransferases (MTases), RapI, RapM and RapQ. これらはmacrocycleの後期修飾に関与している可能性がある。 --- [PMID: 11372782](2001) rapQONMLのdeletion mutantはpipecolateを添加すると、rapamycinの代わりにanalog 16-O-desmethyl-27-desmethoxyrapamycinを産生した。これは、rapNによってコードされるP450 hydroxylaseがC-27に特異的で、rapQとrapM によってコードされるO-methyltransferasesがC-16 and C-27でのhydroxyl基をメチル化することを示す。 -- [PMID: 16990929](2006) rapKIJMNOQL deletion mutant S.hygroscopicus MG2-10をrapMで相補すると、C-16でのみO-methylationされたpre-rapamycin(PKSから放出された後の最初の中間体。未修飾aglycon.)が得られる。また、rapMを発現したS.lividans TK24株にpre-rapamycinを与えると、同じ化合物が得られた。 rapKIJMNOQL deletion mutant S.hygroscopicus MG2-10をrapMNOで相補すると、C-27でhydroxylateされ、C-16でmethylateされたpre-rapamycinを蓄積。よって、RapMはC-16に特異的なO-methylationを担う。

Sequence

>C-16 O-methyltransferase [methyltransferase]
MIQPDVVTAFTAVRDINLDEPGTPQAETKAATFKSASSSIYDLAASMSAKGQLWNWGVHD
SEFVAEIEARLPGFTEYGTDGFSEQLYYLALRDLPKGLESCDGRTVLEVGCGMGEGLNFL
SRLVPTARMTGLDLAPKAIASATATLSRGDTLRFVQGDAEELPFEDSSVDVLVNIESSHT
YPNLGRFLREAARALRRGGALSHIDVFTRQRLEAMRRITTEIPELKWVSDHDISDQVRAA
VRRRMPPDSHLRSTLNKQRMNRLARTLALHSQITVFGGTFADYQPPASVKMLSRLGLVPP
MDSLPMETYRHQIAVRV

>C-16 O-methyltransferase [methyltransferase]
ATGATCCAACCCGACGTCGTGACCGCCTTCACAGCGGTTCGCGACATCAATCTCGACGAA
CCCGGCACCCCGCAGGCGGAGACGAAGGCCGCCACCTTCAAGTCCGCCAGCTCCTCCATC
TACGACCTCGCCGCCAGCATGTCGGCAAAGGGCCAGCTGTGGAACTGGGGTGTGCACGAT
TCCGAGTTCGTCGCCGAGATCGAGGCCAGGCTGCCCGGATTCACCGAGTACGGCACCGAC
GGATTCAGCGAGCAGCTCTACTACCTGGCCCTGCGCGACCTTCCGAAGGGCTTGGAGAGC
TGCGACGGCCGGACGGTCCTGGAGGTCGGCTGCGGAATGGGGGAGGGGCTCAACTTCCTG
TCCCGCCTCGTCCCCACCGCCCGGATGACCGGGCTGGACCTGGCGCCGAAGGCGATCGCC
AGCGCCACCGCCACGCTGTCCCGCGGCGATACCCTGCGGTTCGTCCAGGGCGACGCCGAG
GAACTGCCCTTCGAGGACTCCTCGGTGGATGTGCTCGTCAACATCGAGAGCTCGCACACC
TACCCCAATCTGGGACGTTTCCTGCGGGAGGCCGCGCGCGCGCTGCGCCGGGGTGGCGCC
CTTTCGCACATCGACGTGTTCACGCGGCAGCGCCTTGAGGCGATGCGTCGGATCACAACG
GAAATACCCGAACTGAAGTGGGTCAGCGATCACGACATCTCCGATCAAGTCCGTGCCGCG
GTGCGCCGGCGGATGCCCCCGGACAGTCACCTCCGCAGCACCCTGAACAAACAACGGATG
AACCGGCTTGCGCGCACGCTCGCCCTGCACAGCCAGATCACGGTGTTCGGCGGCACGTTC
GCGGACTACCAGCCGCCCGCTTCGGTCAAAATGCTCAGCCGGCTCGGCCTCGTGCCGCCG
ATGGACAGCCTGCCGATGGAAACCTATCGGCACCAGATCGCCGTCCGCGTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR013216 Methyltransferase type 11 (Domain) PF08241 Methyltransf_11
SignalP	Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Rapam_00170 Rapam_00190