Rapam_00270 Rapam_00270   last last

Rapam_00280 : CDS information

close this sectionLocation

Organism
StrainNRRL 5491
Entry nameRapamycin
Contig
Start / Stop / Direction106,758 / 107,379 / + [in whole cluster]
106,758 / 107,379 / + [in contig]
Location106758..107379 [in whole cluster]
106758..107379 [in contig]
TypeCDS
Length622 bp (206 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.2 function unknown
Productputative pyridoxal phosphate-dependent enzyme(fragment)
Product (GenBank)cystathione synthase
Gene
Gene (GenBank)orfDD
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995)
[8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996)
comment
UniProt submitted nameはCystathione synthase
Sequence status: Fragment

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[PMID: 7644502](1995)
rapamycin生合成gene clusterの報告。配列解析。
ORF DD: 記載なし

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[PMID: 8635730](1996)
PKS flanking genesの配列解析。
多様な真核生物のpyridoxal-phosphate dependent cystathionine synthasesに明らかな配列類似が見られる(>id30%)。
よってここからはrap cluster外と判断している。
Related Reference
ACC
Q2QD00
PmId
[17225146] Identification of three Zwittermicin A biosynthesis-related genes from Bacillus thuringiensis subsp. kurstaki strain YBT-1520. (Arch Microbiol. , 2007)
[18692050] L-2,3-diaminopropionate: one of the building blocks for the biosynthesis of Zwittermicin A in Bacillus thuringiensis subsp. kurstaki strain YBT-1520. (FEBS Lett. , 2008)
comment
9th(Q2QD00) 50%, 6e-55
Bacillus thuringiensis_zwa5A
Zwa5A

zwa5A(-) mutantはZwittermicin A非産生。
zwa5Aでは補完されないが、zwa5A-5Bでは補完されZwittermicin A産生回復。polar effectの可能性あり。
2,3-diaminopropionate添加でもZwittermicin A産生回復。

NRPS ORF3がのadenylation domain ZWAA2がL-2,3-diaminopropionateを効率的にacyl-adenylateした。
よってL-2,3-diaminopropionateは実際にZwittermicin A生合成の伸長unitである。
Family/Domain
FD
IPR001926
comment
[IPR001926] Pyridoxal phosphate-dependent enzyme, beta subunit (Domain)

close this sectionSequence

selected fasta
>putative pyridoxal phosphate-dependent enzyme(fragment) [cystathione synthase]
MGTPLIPLADEQCDIVAKLEFCNPTGSSKDRSALWILEQAIGRGEIARSTTVVESSSGNF
ALALAFYCRMLGISFVPVIDPNCNEATEAQLRLLCERVEKVAIRDGSGSYLKNRLSRVRE
LLVELDSAYWPNQYANPDARDAHYHFTAGELIAQAGPLDYLFVGVGTGGTIAGLSRRIKE
TYPDCVVVAVDAEGSVIFGGPPKKRRI-
selected fasta
>putative pyridoxal phosphate-dependent enzyme(fragment) [cystathione synthase]
ATGGGAACCCCGCTGATTCCTCTGGCGGACGAGCAGTGTGACATCGTCGCGAAGCTGGAA
TTCTGCAATCCCACGGGAAGCAGCAAAGACCGTTCGGCGCTGTGGATTCTGGAACAGGCG
ATCGGCCGCGGGGAGATAGCGCGGAGCACGACGGTGGTCGAGTCCTCGTCCGGCAATTTC
GCGCTGGCCCTGGCCTTCTACTGCCGGATGCTCGGCATCTCGTTCGTCCCGGTCATCGAT
CCGAACTGCAACGAGGCCACCGAGGCCCAGCTCCGGCTGCTGTGCGAGCGGGTGGAGAAA
GTCGCCATCCGTGACGGGTCGGGCAGCTACCTGAAGAACCGGCTGTCCCGGGTCCGGGAA
CTGCTGGTCGAACTCGACTCGGCCTATTGGCCCAACCAGTACGCCAATCCCGATGCCCGC
GACGCGCATTACCACTTCACCGCGGGCGAGCTGATCGCACAGGCCGGCCCGCTGGATTAT
CTCTTCGTCGGCGTGGGCACCGGCGGCACGATCGCCGGGCTCTCCCGCCGGATCAAGGAA
ACCTACCCCGATTGCGTCGTCGTCGCCGTCGACGCCGAAGGCTCGGTGATCTTCGGCGGC
CCGCCGAAGAAGCGGCGGATCC

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001926 Pyridoxal phosphate-dependent enzyme, beta subunit (Domain)
[2-198] 9.8e-39 PF00291
PF00291   PALP
[3-207] 1.9e-46 SSF53686
SSF53686   Tryptophan synthase beta subunit-like PLP-dependent enzymes
SignalP No significant hit
TMHMM No significant hit
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