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Rubra_00010 : CDS information

close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction933 / 1 / - [in whole cluster]
933 / 1 / - [in contig]
Locationcomplement(1..933) [in whole cluster]
complement(1..933) [in contig]
TypeCDS
Length933 bp (310 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter ATP-binding protein
Product (GenBank)putative ABC transporter
Gene
Gene (GenBank)rubT1
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
comment
Rubradirin生合成clusterの報告。
RubT1(320aa): ABC transporter(ATP binding)

Rubradirinの輸送に関与すると推測される。
Related Reference
ACC
Q53716
PmId
[8326867] Streptomyces antibioticus contains at least three oleandomycin-resistance determinants, one of which shows similarity with proteins of the ABC-transporter superfamily. (Mol Microbiol. , 1993)
comment
275th(Q53716) 42%, 4e-51
Streptomyces antibioticus_oleC-ORF4
ATP-binding protein

S. antibioticsでoleandomycinに抵抗性を示す遺伝子を単離。
OleC plasmidでクローニングされたORFが5つあり、
Orf4 (NBD), Orf5(TMD)がABC transorterとして働いているのではないかと推論。
ACC
Q9F5K4
NITE
Avil_00350
PmId
[11181344] An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57. (Antimicrob Agents Chemother. , 2001)
comment
297th(Q9F5K4) 42%, 5e-51
Streptomyces viridochromogenes_aviABC1
Putative ATP binding protein

S. lividans TK66にてaviABC1, aviABC2, aviRa, aviRb発現。
--> avilamycin resistance
(avilamycin A inhibited poly(U)-directed polyphenylalanine synthesis)

close this sectionSequence

selected fasta
>putative ABC transporter ATP-binding protein [putative ABC transporter]
MEKIYPASGSRPEVPAVRGIDLDVGRGEFFGLLGPNGAGKSTTIGMLTTLIVPTGGSAEV
YGVDVVRNPIEVKRRIGVVSQNNTLDSQLTAAENLEFRGRYFGLTAKEARRRTDELIELF
DLAEQREGNPFEMSGGQAKRVMICRALMHRPDALFLDEPTAGLDPQTRTNLWQVLRELQA
GGQTILLTTHYMEEAEALCDRIAVVDHGKILARGTVDELKSIAGADTVITVSYDAPVAPA
GLQALADRDGISKVEVSDRQVRVFATVPDGLLGELVTIGSAAGIGVTDVHQLRPSLETAF
LTLTGRSYRE
selected fasta
>putative ABC transporter ATP-binding protein [putative ABC transporter]
ATGGAGAAGATTTACCCGGCCTCCGGTTCGCGGCCCGAGGTGCCCGCCGTCCGCGGCATC
GACCTGGACGTGGGACGCGGGGAGTTCTTCGGCCTTCTCGGCCCCAACGGCGCGGGCAAG
TCGACCACCATCGGGATGCTCACCACCCTGATCGTGCCGACCGGCGGCAGCGCCGAGGTG
TACGGGGTCGATGTGGTGCGCAACCCGATCGAGGTCAAGCGGCGGATCGGGGTGGTATCG
CAGAACAACACCCTGGACAGCCAGTTGACCGCCGCCGAGAACCTGGAGTTCCGGGGCCGC
TACTTCGGGCTCACCGCCAAAGAGGCACGCCGCCGCACGGACGAGCTGATCGAACTGTTC
GACCTCGCCGAGCAGCGCGAGGGCAATCCGTTCGAGATGTCCGGCGGACAGGCCAAGCGA
GTGATGATCTGCCGGGCGCTGATGCACCGCCCCGATGCGCTCTTCCTGGACGAGCCGACT
GCCGGACTGGACCCGCAGACCCGTACCAACCTGTGGCAGGTACTGCGAGAGCTGCAGGCG
GGCGGCCAGACGATCCTGCTCACCACGCACTACATGGAAGAGGCCGAGGCCCTGTGCGAC
CGCATCGCGGTTGTCGACCACGGCAAGATCCTCGCGCGCGGCACGGTGGACGAGCTGAAG
AGCATCGCAGGCGCCGACACCGTCATCACCGTCAGCTACGACGCGCCCGTTGCCCCCGCC
GGACTCCAGGCCCTCGCCGACCGGGACGGGATCAGCAAGGTCGAGGTCAGTGACAGGCAG
GTGCGGGTGTTCGCCACCGTTCCGGACGGACTGCTCGGCGAGCTCGTGACGATCGGCTCG
GCCGCCGGGATCGGCGTGACCGATGTGCACCAGCTGCGCCCCAGCCTGGAAACTGCGTTC
CTCACGTTGACCGGAAGGAGCTACCGGGAATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003439 ABC transporter-like (Domain)
[41-161] 6.79999999999998e-18 PF00005
PF00005   ABC_tran
[2-232] PS50893
PS50893   ABC_TRANSPORTER_2
IPR003593 AAA+ ATPase domain (Domain)
[26-208] 3.0999994589937e-14 SM00382
SM00382   AAA
IPR005894 Daunorubicin resistance ABC transporter ATP-binding subunit (Family)
[13-305] 2.70000000000002e-94 TIGR01188
TIGR01188   DrrA
IPR017871 ABC transporter, conserved site (Conserved_site)
[133-147] PS00211
PS00211   ABC_TRANSPORTER_1
SignalP
[1-58] 0.058 Signal
Eukaryota   
TMHMM No significant hit
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