Rubra_00100 Rubra_00100   Rubra_00120 Rubra_00120

Rubra_00110 : CDS information

close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction26,018 / 27,583 / + [in whole cluster]
26,018 / 27,583 / + [in contig]
Location26018..27583 [in whole cluster]
26018..27583 [in contig]
TypeCDS
Length1,566 bp (521 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productaminocoumarin--carboxylic acid ligase
amide synthetase
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number6.3.1.-
Keyword
Note
Note (GenBank)
  • ORF4
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
[21448870] Adenylate-forming enzymes of rubradirin biosynthesis: RubC1 is a bifunctional enzyme with aminocoumarin acyl ligase and tyrosine-activating domains. (Chembiochem. , 2011)
comment
[PMID: 18080113](2008)
Rubradirin生合成clusterの報告。
ORF4(521aa): Aminocoumarin ligase

本文に詳細記載なし。

---
[PMID:21448870](2011)
aminocoumarin生合成、結合に関連するputative acyladenylate-forming enzymesの候補、Orf4, RubF6, RubC1を発現、精製して調査している。
Orf4 (55 kDa)は活性のあるaminocoumarin acyl ligaseである。

Rubradirin生合成において、Orf4と、RubC1のN末domainの両方がaminocoumarin acyl ligasesとして働くことができるかもしれない。
RubC1, C2, C3の並びはnovobiocin生合成clusterでのsingle operon NovHIJKLに似ていることから、RubC1のほうが効率的でありうる。
Related Reference
ACC
Q9L9F6
PmId
[10801869] Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891. (J Biol Chem. , 2000)
comment
12th(Q9L9F6) 36%, 4e-70
Streptomyces sphaeroides_novL
NovL

Novobiocic acid synthetaseをコードするnovLをE.coliで発現、精製、活性測定。
3-dimethylallyl-4-hydroxybenzoic acid (ring A of novobiocin) と
3-amino-4,7-dihydroxy-8-methyl coumarin (ring B of novobiocin)
の間のアミド結合の形成をATP-dependent reactionで触媒した。
また、ring A and ATPからのacyl adenylateの形成がATP-PP(i) exchange assayによって実証された。
すなわちNovLは、acyl adenylateのようなring Aの活性化と、引き続くacyl基のring Bのamino基への移動の両方を触媒した。
ACC
Q2PC59
NITE
Rubra_00310
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
[21448870] Adenylate-forming enzymes of rubradirin biosynthesis: RubC1 is a bifunctional enzyme with aminocoumarin acyl ligase and tyrosine-activating domains. (Chembiochem. , 2011)
comment
2nd, id85%, 0.0
Rubradirin生合成cluster内homolog
RubF6: Aminocoumarin ligase

---
[PMID:21448870](2011)
aminocoumarin生合成、結合に関連するputative acyladenylate-forming enzymesの候補、Orf4, RubF6, RubC1を発現、精製して調査している。
RubF6 (56 kDa)は不活性だが、site-directed mutagenesisにより活性型酵素へ変換されうる。

close this sectionSequence

selected fasta
>aminocoumarin--carboxylic acid ligase [hypothetical protein]
MSTTHVHYVQQLLTHFDADPDRPAMVSGDVPFSAGELADAVRRAAAAMGRHGVGRGDVVC
ILTEPNTAATLILRWAANLVGATAAHVRGMNAVVPDDELRMDLQRAIVSDVGARMLAVDP
ANEARARELLVNATGRPVLAVLGAGQPDTVDLTAGCGDGVGPCPDITDSDLAVITQTSGA
SGLPKGVCWPFGVKNDMAASAIDRSSRTNVLITAPLTHSSGFAADDTLITGGMVVLHPGF
DAAAVLHAIAQHRIGRLILGTPQVYALAEHPDRAATDLSSLTELIYTGSPGAPLKLRKAR
EIFGPVLIQVYGTTEDRSAHMLPRRHEHLRACSSAGRPVNPEALSIRHPDTGAVLPVGEV
GEVCAVPRWPTAGYWHEPALTAALVRDGWVRTGDLGHLDTDGYLHLTGRLANMMKVKGIR
IHPEQVEKVLRQAPGVSQAAVCGVEDADRVEHIYAAVVPEPGADPDPRELRRHVAEALSD
TYVPRLIDIRRKLPTTGWDKPDRVRLRADARAALTRPAPQA
selected fasta
>aminocoumarin--carboxylic acid ligase [hypothetical protein]
ATGAGTACCACGCATGTGCATTATGTTCAGCAGCTGCTGACTCATTTCGATGCCGATCCG
GATCGCCCTGCCATGGTCAGCGGGGACGTCCCCTTCTCCGCCGGGGAGCTGGCCGACGCC
GTTCGCCGGGCCGCGGCCGCGATGGGCCGCCACGGCGTCGGCCGCGGTGACGTCGTCTGC
ATCCTGACCGAGCCCAACACCGCGGCCACACTGATCCTGCGGTGGGCGGCCAACCTGGTG
GGGGCGACCGCCGCACATGTGCGTGGGATGAATGCGGTGGTGCCTGACGACGAGTTGCGC
ATGGACTTGCAGCGCGCCATCGTCTCGGATGTCGGCGCCCGGATGCTGGCGGTCGACCCG
GCCAACGAGGCACGGGCACGGGAGCTGCTGGTGAACGCGACCGGTCGGCCGGTCCTGGCC
GTACTGGGCGCAGGACAGCCGGACACGGTGGATTTGACCGCCGGCTGCGGTGACGGAGTA
GGCCCGTGCCCGGACATCACCGACAGCGACCTCGCGGTGATCACCCAGACCAGTGGGGCG
AGCGGCCTGCCGAAGGGTGTCTGCTGGCCCTTCGGCGTCAAGAACGACATGGCCGCATCG
GCCATCGACCGCAGCAGCCGGACGAACGTCCTGATCACCGCACCGCTCACCCATTCCAGC
GGGTTCGCCGCGGACGACACGCTCATCACCGGCGGCATGGTCGTCCTGCATCCCGGCTTC
GACGCCGCGGCCGTGCTCCACGCGATCGCGCAGCACCGCATCGGCCGGCTCATCCTCGGC
ACCCCGCAGGTGTACGCACTGGCCGAGCACCCGGACCGGGCGGCCACCGACCTGTCCAGC
CTGACCGAGCTGATCTACACCGGCAGCCCCGGGGCACCGCTCAAGCTGCGCAAGGCCCGG
GAAATCTTCGGCCCCGTACTGATCCAGGTCTACGGCACGACCGAAGACCGGAGTGCTCAC
ATGCTCCCCCGGCGACACGAACACCTGCGCGCCTGCTCCAGCGCGGGCCGGCCGGTCAAC
CCAGAGGCGCTCAGCATCCGGCACCCCGACACCGGCGCGGTACTGCCCGTCGGCGAGGTC
GGCGAGGTCTGCGCGGTTCCACGCTGGCCCACGGCCGGCTACTGGCACGAGCCGGCACTC
ACCGCGGCACTCGTCCGCGACGGCTGGGTGCGCACCGGCGACCTCGGGCACCTCGACACC
GACGGCTACCTGCACCTGACCGGCCGGCTCGCGAACATGATGAAGGTCAAGGGAATCCGC
ATCCACCCCGAGCAGGTGGAGAAGGTGCTCAGGCAGGCCCCGGGCGTGTCCCAGGCCGCC
GTATGCGGGGTCGAGGACGCCGACCGGGTGGAGCACATCTACGCCGCCGTCGTGCCCGAG
CCCGGCGCCGACCCGGATCCCCGGGAACTGCGCCGTCACGTCGCCGAGGCACTGTCCGAC
ACCTACGTCCCCCGCCTGATCGACATTCGCCGGAAACTGCCGACGACCGGCTGGGACAAA
CCCGATCGGGTCCGGCTGCGGGCCGACGCCCGCGCCGCACTGACCCGGCCCGCCCCCCAG
GCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[35-441] 7.00000000000005e-59 PF00501
PF00501   AMP-binding
SignalP No significant hit
TMHMM No significant hit
Rubra_00100 Rubra_00100   Rubra_00120 Rubra_00120
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